[1]任校峰,李世荣,王珍祥,等.NO合剂对糖尿病小鼠创面愈合的促进作用[J].陆军军医大学学报(原第三军医大学学报),2010,32(02):150-154.
 Ren Xiaofeng,Li Shirong,Wang Zhenxiang,et al.NO mixture promotes wound healing of diabetes mellitus mice[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(02):150-154.
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NO合剂对糖尿病小鼠创面愈合的促进作用(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第02期
页码:
150-154
栏目:
论著
出版日期:
2010-01-30

文章信息/Info

Title:
NO mixture promotes wound healing of diabetes mellitus mice
作者:
任校峰李世荣王珍祥于攀
第三军医大学西南医院整形外科
Author(s):
Ren Xiaofeng Li Shirong Wang Zhenxiang Yu Pan
Department of Plastic Surgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
关键词:
NO糖尿病创面愈合精氨酸夹竹桃麻素硝普钠
Keywords:
NO diabetes mellitus wound healing arginine apocynin sodium nitroferricyanide
分类号:
R587.1;R641.02;R982
文献标志码:
A
摘要:
目的  研究NO合剂(硝普钠、夹竹桃麻素、精氨酸)对糖尿病小鼠皮肤创面愈合的影响。  方法  50 只雄性昆明小鼠腹腔注射链脲佐菌素以建立糖尿病小鼠模型,建模成功后按完全随机分组的方法分成对照组、L-精氨酸(L-Arg)治疗组、夹竹桃麻素(APO)治疗组、硝普钠(SNP)治疗组及NO合剂治疗组,每组10只小鼠。在小鼠背部建立创面,分别以相应药物创面下注射。创面动态摄像并于相应时间段取标本,观察创面组织愈合情况,计算创面愈合率;免疫组化染色检测创面成纤维细胞密度、胶原纤维面密度和血管密度。  结果  创面形成后第3天起,各药物治疗组小鼠创面愈合率较对照组明显上升(P<0.05,P<0.01), 以创面形成后7 d 内变化最为明显。与对照组相比,各药物治疗组小鼠的创面成纤维细胞密度、胶原面密度、血管密度均明显升高(P<0.05,P<0.01),其中NO合剂治疗组与对照组比较差异更显著,L-Arg、APO、SNP治疗组之间比较差异无统计学意义(P>0.05)。  结论  NO合剂可有效促进糖尿病小鼠创面愈合,较L-Arg、APO、SNP单独使用效果更为显著。
Abstract:
Objective  To investigate the influence of NO mixture (L-arginine, apocynin and sodium nitroferricyanide) on wound healing of diabetes mellitus (DM) mice. Methods  Fifty adult male Kunming mice were induced by streptozotocin to establish type I DM model. They were then randomly divided into control group, L-arginine group (150 g/L), apocynin group (1×10-4 mol/L), sodium nitroferricyanide group (0.1 mmol/L) and NO mixture group (above components were mixed with equal ratio). Full-thickness skin wound was made and injected with corresponding drugs of 0.15 ml once every 2 days. The wounds were digitally photographed to calculate the percentage of wound closure using computer image analysis software in 1, 3, 5, 7 and 10 d post-injury. The density of fibroblasts, the content of collagenous fibers and the neovascularization in the wound samples were measured with the aid of HE staining. Results  From the third day post-injury, the wound healing rate of NO mixture group was significantly higher than that of all the other groups (P<0.01). Compared with control group, the density of fibroblasts, the content of collagenous fibers and the neovascularization were all increased in medicine-injected group  (P<0.05,P<0.01). However, the difference between NO mixture group and the control group was the most significant one among all the groups. Conclusion  NO mixture effectively enhances the wound healing of DM mice, and its promotion is much more significant than that of L-arginine, apocynin and sodium nitroferricyanide used alone.

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更新日期/Last Update: 2010-01-21