[1]刘颖,林中,胡琼花,等.伴有胃肠动力障碍的重症急性胰腺炎大鼠结肠肠肌间神经丛一氧化氮合成酶神经元的变化[J].第三军医大学学报,2010,32(03):274-277.
 Liu Ying,Lin Zhong,Hu Qionghua,et al.Change of nitric oxide synthase neurons in colonic myenteric plexus of rats with severe acute pancreatitis complicated by gastrointestinal dysmotility[J].J Third Mil Med Univ,2010,32(03):274-277.
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伴有胃肠动力障碍的重症急性胰腺炎大鼠结肠肠肌间神经丛一氧化氮合成酶神经元的变化(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第03期
页码:
274-277
栏目:
论著
出版日期:
2010-02-15

文章信息/Info

Title:
Change of nitric oxide synthase neurons in colonic myenteric plexus of rats with severe acute pancreatitis complicated by gastrointestinal dysmotility
作者:
刘颖林中胡琼花郑清华
桂林医学院附属医院消化科
Author(s):
Liu Ying Lin Zhong Hu Qionghua Zheng Qinghua
Department of Gastroenterology, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi Zhuang Autonomous Region, 541001,China
关键词:
重症急性胰腺炎胃肠动力障碍肠神经系统肌间神经丛一氧化氮合成酶神经元
Keywords:
severe acute pancreatitisgastrointestinal dysmotilityenteric nervous systemmyenteric plexusnitric oxide synthase neuron
分类号:
R322.85;R329.24;R576.02
文献标志码:
A
摘要:
目的  观察伴有胃肠动力障碍的重症急性胰腺炎(severe acute pancreatitis,SAP)大鼠结肠肠神经系统(enteric nervous system,ENS)肌间神经丛一氧化氮合成酶(nitric oxide synthase,NOS)神经元的变化,以探讨SAP胃肠动力障碍的神经机制。  方法  20只SD大鼠随机均分为假手术组和SAP组,逆行胰胆管穿刺逆行注射5%牛磺胆酸钠制作SAP模型。检测腹部X线、小肠推进比,胰腺病理评分。制作肌间神经丛全层标本,应用双重免疫荧光染色法观察NOS神经元的形态及占总神经元的百分比。  结果  与假手术组相比,SAP组大鼠肠管明显扩张,SAP组小肠推进比显著降低(P<0.01),胰腺病理评分明显增高(P<0.01),NOS神经元胞体大而染色深,结间束NOS神经纤维粗大,NOS神经元比例为(40.74±5.15)%明显高于假手术组(P<0.01)。  结论  结肠肌间丛NOS神经元表达增多可能是大鼠SAP胃肠动力障碍神经机制之一。
Abstract:
Objective  To study the nervous mechanism underlying gastrointestinal dysmotility by observing the change of nitric oxide synthase(NOS) neurons in colonic myenteric plexus of the enteric nervous system (ENS)in rats with severe acute pancreatitis (SAP)complicated by gastrointestinal dysmotility.   Methods  Twenty Sprague Dawley rats were randomly divided into sham operation group and SAP group. A SAP model was induced by retrograde cholangiopancreatography-guided injection of 5% sodium taurocholate. Abdominal X-ray findings and small intestinal impelling ratio were detected. Pancreatic lesion was scored. Whole mount samples of myenteric plexus were prepared and stained with double immunofluorescence to observe the morphology of NOS neurons. The percentage of NOS neurons in the total neurons was calculated.   Results  The intestine was much more expanded, the small intestinal impelling ratio was significantly lower (P<0.01), the score of pancreatic lesion was remarkably higher (P<0.01), the NOS neurons were bigger and more deeply stained, and the nerve fibers between ganglions of the NOS were thicker in SAP group than in sham operation group. The percentage of NOS neurons was (40.74±5.15)% in SAP group, which was significantly higher than that in sham operation group (P<0.01).   Conclusion  A high expression level of NOS neurons in colonic myenteric plexus may be one of the nervous mechanisms underlying gastrointestinal dysmotility in SAP rats.

参考文献/References:

刘颖,林中,胡琼花,等.伴有胃肠动力障碍的重症急性胰腺炎大鼠结肠肠肌间神经丛一氧化氮合成酶神经元的变化[J]. 第三军医大学学报,2010,32(3):274-277.

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更新日期/Last Update: 2010-01-27