[1]候艳,陈全,王瑜伟,等.Ipr1重组卡介苗对小鼠结核病的治疗效果[J].第三军医大学学报,2009,31(22):2262-2265.
 HOU Yan,CHEN Quan,WANG Yu-wei,et al.Therapeutic effects of murine Ipr1 recombinant BCG vaccine on Mycobacterium tuberculosis infection in murine model[J].J Third Mil Med Univ,2009,31(22):2262-2265.
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Ipr1重组卡介苗对小鼠结核病的治疗效果(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第22期
页码:
2262-2265
栏目:
论著
出版日期:
2009-11-30

文章信息/Info

Title:
Therapeutic effects of murine Ipr1 recombinant BCG vaccine on Mycobacterium tuberculosis infection in murine model
作者:
候艳陈全王瑜伟严莎吕玉春郭荫广
重庆医科大学分子医学与肿瘤研究中心,基础医学院免疫学教研室
Author(s):
HOU Yan CHEN Quan WANG Yu-wei YAN Sha LU Yu-chun GUO Yin-guang
Molecular Medicine and Tumor Research Center, Department of Immunology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China
关键词:
细胞内病原体抗性基因1结核分枝杆菌卡介苗
Keywords:
Ipr1Mycobacterium tuberculosis BCG vaccine
分类号:
R392.7;R521.05;R969.4
文献标志码:
A
摘要:
目的  评估携带小鼠胞内病原体抗性基因1(intracellular pathogen resistance 1, Ipr1)质粒(pBOGI)的重组卡介苗(BCG)对结核分枝杆菌(Mtb)感染的治疗效果。  方法  BALB/c小鼠30只,随机分为3组:分别滴鼻给予磷酸盐缓冲液(PBS)、BCG和重组BCG,2周后用人型Mtb H37Rv标准株感染所有小鼠;感染后分别用PBS、BCG和重组BCG治疗7次;末次治疗后处死小鼠,检测肺脾荷菌量,肺脾脏器指数,血清IFN-γ、 IL-10及TNF-α分泌水平和肺脾组织中Ipr1的表达,同时观察小鼠肺脾组织病理改变情况。  结果  重组BCG组肺脾荷菌量比PBS组和BCG组显著降低(P<0.01);重组BCG组肺脾脏器指数比PBS组和BCG组显著降低(P<0.05);重组BCG组血清IFN-γ 分泌水平比PBS组和BCG组显著升高(P<0.01)。用免疫组化检测到小鼠肺脾组织中有Ipr1表达。PBS组肺组织病理改变以渗出为主,病变广泛;重组BCG组肺部病变最轻,肺泡结构基本正常;BCG组病变范围局限,病变程度界于PBS组与重组BCG组之间;各组间比较脾脏病理改变不明显。  结论  携带小鼠Ipr1基因的重组BCG对结核分枝杆菌感染有一定的治疗作用。
Abstract:
Objective   To evaluate the effects of recombinant BCG vaccine encoding murine Ipr1 on Mycobacterium tuberculosis infection in rats.    Methods   Thirty BALB/c mice were randomized into 3 groups which were intranasally given PBS, BCG and recombinant BCG respectively. Two weeks later, the 3 groups were infected with Mycobacterium tuberculosis and were intranasally treated with PBS, BCG, recombinant BCG for once per 3 d, 7 times totally respectively. The mice were sacrificed after the final treatment. The viable quantity of bacteria in the lungs and the spleens was detected. The weight indexes of the lungs and the spleens were calculated. ELISA was used to detect the levels of IL-10, TNF-α and IFN-γ in the serum. The expression of Ipr1 in lung and spleen tissues was detected by immunohistochemistry. Lungs and spleens were prepared for pathological analysis.    Results   In the recombinant BCG group, significant reduce was observed in number of colony forming units and weight indexes of lungs and spleens compared with the PBS group and the BCG group in the same murine strain. The level of IFN-γ was significantly increased in the recombinant BCG group compared with the other 2 groups. The expression of Ipr1 in the tissues was found in the recombinant BCG group. The pathological changes in lungs of the recombinant BCG group and the BCG group were localized, while those in the PBS group were extensive. There was no significant changes in the spleen of all groups.   Conclusion   The recombinant BCG encoding murine Ipr1 has therapeutic effects on murine Mycobacterium tuberculosis infection.

参考文献/References:

候艳,陈全,王瑜伟,等.Ipr1重组卡介苗对小鼠结核病的治疗效果[J]. 第三军医大学学报,2009,31(22):2262-2265.

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更新日期/Last Update: 2009-11-23