[1]孟景山,司道文,张作凤,等.孕酮通过NF-κB调控脑损伤后大鼠海马神经元COX-2和Caspase-3的表达[J].第三军医大学学报,2010,32(02):146-149.
 Meng Jingshan,Si Daowen,Zhang Zuofeng,et al.Progesterone regulates COX-2 and Caspase-3 expressions in hippocampal neurons in rats after traumatic brain injury by NF-κB signaling pathway[J].J Third Mil Med Univ,2010,32(02):146-149.
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孕酮通过NF-κB调控脑损伤后大鼠海马神经元COX-2和Caspase-3的表达(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第02期
页码:
146-149
栏目:
论著
出版日期:
2010-01-30

文章信息/Info

Title:
Progesterone regulates COX-2 and Caspase-3 expressions in hippocampal neurons in rats after traumatic brain injury by NF-κB signaling pathway
作者:
孟景山司道文张作凤张宇新张子明
华北煤炭医学院基础医学部解剖学教研室
Author(s):
Meng Jingshan Si Daowen Zhang Zuofeng Zhang Yuxin Zhang Ziming
Department of Anatomy, North China Coal Medical College, Tangshan, Hebei Province, 063000, China
关键词:
脑损伤孕酮核因子-κB环氧合酶-2半胱氨酸天冬氨酸蛋白酶-3
Keywords:
traumatic brain injury progesterone nuclear factor-κB cyclooxygenase-2 Caspase-3
分类号:
R651.15;R345;R977.12
文献标志码:
A
摘要:
目的  研究NF-κB对脑损伤大鼠海马神经元环氧合酶-2(COX-2)、半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)的表达调控作用,探讨孕酮(progesterone,PROG)对脑损伤(TBI)后大鼠海马神经元保护作用及其可能机制。  方法  雄性SD大鼠45只按抛硬币法分为假手术组、损伤组和PROG组,按照改良的Feeney自由落体损伤装置制作大鼠脑损伤模型,用Nissl染色法、免疫组织化学法及免疫蛋白印记法,观察大鼠伤侧海马CA1区NF-κB、COX-2以及Caspase-3的表达变化和孕酮对上述指标变化的影响。  结果  损伤组大鼠海马CA1区NF-κB(24.0±2.5)、COX-2(35.9±2.7)、Caspase-3(25.1±2.7)阳性神经元的数量较假手术组NF-κB(1.9±0.9)、COX-2(1.5±0.7)、Caspase-3(1.8±0.8)显著增加(P<0.05);PROG组与损伤组比较,海马CA1区NF-κB(14.2±1.8)、COX-2(16.6±2.7)、Caspase-3(11.2±2.4)阳性神经元的数量明显减少(P<0.05)。  结论  NF-κB对脑损伤后大鼠海马神经元COX-2和Caspase-3表达起调控作用;PROG通过NF-κB对脑损伤后大鼠海马神经元起保护作用。
Abstract:
Objective  To investigate the effect of nuclear factor-κB (NF-κB) on the regulation of cyclooxygenase-2 (COX-2) and Caspase-3 in hippocampal neurons after traumatic brain injury (TBI) in rats, and explore the neuroprotective effect and the possible mechanism of progesterone (PROG) in hippocampal neurons after TBI. Methods  Forty-five male Spraque-Dawley rats were randomly divided into 3 groups: sham-operated group (n=15), TBI group (n=15) and PROG-treated group (n=15, intraperitoneal injection of PROG 16 mg/kg in 1 and 6 h after injury). The rat model of TBI was duplicated with the improved Feeney’s method. The rats were sacrificed in 24 h after injury and their brain was resected. Nissl staining, immunohistochemical staining and Western blot assay for NF-κB, COX-2 and Caspase-3 was used to observe the changes of positive cell numbers and protein levels in the hippocampal neurons. Results  The numbers of immunoreactive neurons to NF-κB (24.0±2.5), COX-2 (35.9±2.7) and Caspase-3 (25.1±2.7) were significantly increased in the hippocampus at 24 h after TBI when compared with the positive neuron numbers of NF-κB (1.9±0.9), COX-2 (1.5±0.7) and Caspase-3 (1.8±0.8) in sham group. After the treatment of PROG, the positive cell number of NF-κB (14.2±1.8), COX-2 (16.6±2.7), Caspase-3 (11.2±2.4) was reduced obviously as compared with the TBI group (P<0.05). Conclusion  NF-κB signaling pathway may play an important role in the regulation of COX-2 and Caspase-3 in the hippocampus neurons after TBI in rats. PROG may influence the expression of NF-κB and protect the hippocampal neurons.

参考文献/References:

孟景山,司道文,张作凤,等.孕酮通过NF-κB调控脑损伤后大鼠海马神经元COX-2和Caspase-3的表达[J]. 第三军医大学学报,2010,32(2):146-149.

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更新日期/Last Update: 2010-01-21