[1]廖炀,陈军华,朱朝敏,等.重庆婴幼儿轮状病毒分子流行病学特征及相关临床资料分析[J].陆军军医大学学报(原第三军医大学学报),2010,32(01):77-80.
 Liao Yang,Chen Junhua,Zhu Chaomin,et al.Molecular epidemiologic characterization and related data analysis of rotavirus in infants of Chongqing[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(01):77-80.
点击复制

重庆婴幼儿轮状病毒分子流行病学特征及相关临床资料分析(/HTML )
分享到:

陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第01期
页码:
77-80
栏目:
论著
出版日期:
2010-01-15

文章信息/Info

Title:
Molecular epidemiologic characterization and related data analysis of rotavirus in infants of Chongqing
作者:
廖炀陈军华朱朝敏许红梅刘作义
重庆医科大学附属儿童医院感染消化科
Author(s):
Liao Yang Chen Junhua Zhu Chaomin Xu Hongmei Liu Zuoyi
Department of Infection and Digestion, Children’s Hospital, Chongqing Medical University, Chongqing, 400014, China
关键词:
腹泻婴幼儿轮状病毒感染轮状病毒属基因型
Keywords:
rotavirus infections genotype diarrhea infants
分类号:
R725.125;R181.33;R373.2
文献标志码:
A
摘要:
目的 研究重庆地区婴幼儿轮状病毒的分子流行病学特征和相关临床资料分析。  方法 收集2008年10月至2009年2月在重庆儿童医院就诊的患非细菌性急性腹泻的婴幼儿大便和问卷。先用胶体金法检测轮状病毒抗原,并经RT-PCR(逆转录PCR)方法证实为轮状病毒,然后用巢式RT-PCR方法进行VP7(G)、VP4(P)基因分型并分析相关临床资料。  结果 共收集到507份大便标本,胶体金法检测轮状病毒抗原阳性率50.69%(257/507)。147份轮状病毒VP6基因阳性标本中G分型结果:G1型占40.14%(59/147);G2型6.80%(10/147);G3型17.01%(25/147);G4型2.04%(3/147);G9型0.68%(1/147);尚有49份未能分型,占33.33%(49/147)。P分型结果:P[8]型占60.54%(89/147);P[4]型占6.12%(9/147);P[9]型和P[10]型各有1例,分别占0.68%(1/147);尚有47份未能分型。G、P型别组合最常见的是G1/P[8]型,占58.93%(33/56)。各G、P型别间临床症状比较无统计学差异。  结论 G、P分型结果与其他地区明显不同,G分型显示出多样性特点,P分型仍以P[8]型占优势。
Abstract:
Objective  To explore the molecular epidemiological characterization and analyze the rela-ted clinical data of rotavirus diarrhea among infants in Chongqing region. Methods  Fecal specimens and questionnaires were collected from infants with non-bacterial acute diarrhea who were admitted at Children’s Hospital of Chongqing Medical University from October 2008 to February 2009. Nested reverse transcription polyme-rase chain reaction (nested RT-PCR) method was used for rotavirus-positive specimens which been verified by screening method with colloidal gold and proved by RT-PCR in order to analyze the VP7 and VP4 genotypes (G- and P-typing). All the results and clinical questionnaires were analyzed. Results  A total of 507 fecal specimens were collected. Rotavirus antigen positive rate was 50.69% (257/507). Out of the 147 rotavirus-positive specimens, G1-type accounts for 40.14%(59/147), G2 6.80%(10/147), G3 17.01%(25/147), G4 2.04%(3/147), and G9 0.68%(1/147), respectively. While 49 cases (33.33%) were failed to detect G-genotypes. Correspondingly, P[8] type accounted for 60.54%(89/147), P[4] 6.12%(9/147), P[9] and P[10] each of 0.68%(1/147), respectively, and 47 cases of failed P-genotyping. Conclusion  Comparing with other areas, the constitutions of rotavirus G- and P-genotypes is different distinctly, and G-genotype shows the characterization of diversity in Chongqing region, however P[8]-genotype is still dominant. However, no significant different result is found when we compare different symptoms about acute diarrhea among almost every G and P-genotypes or G/P combinations.

相似文献/References:

[1]颜婷,孙萍,陈婷婷,等.IL-4、IFN-γ及LTE4浓度在婴幼儿喘息性疾病中的变化及其意义[J].陆军军医大学学报(原第三军医大学学报),2012,34(18):1873.
 Yan Ting,Sun Ping,Chen Tingting,et al.Change and significance of serum IL-4 and IFN-γ and urinary leukotriene E4 in infants with asthmatic diseases[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(01):1873.
[2]孙国林,李生德,齐萍,等.婴幼儿体外循环期间氨甲环酸血液浓度研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(01):69.
 Sun Guolin,Li Shengde,Qi Ping,et al.Study of plasma concentration of tranexamic acid during cardiopulmonary bypass in infants with tetralogy of Fallot[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(01):69.
[3]谢肇,吴雪晖,周强.婴幼儿胸8棘突骨软骨瘤1例[J].陆军军医大学学报(原第三军医大学学报),2007,29(07):634.
[4]王世伟,唐志贵,周世明,等.奇异变形杆菌所致的集体食物中毒病原检测结果分析[J].陆军军医大学学报(原第三军医大学学报),2006,28(21):2201.
[5]潘征夏,杨杰先,吴春,等.钢板胸骨悬吊术矫治婴幼儿漏斗胸的效果[J].陆军军医大学学报(原第三军医大学学报),2006,28(14):1509.
[6]王克霞,朱玉霞,陈琳.腹泻患者中溶组织内阿米巴感染的实验检测分析[J].陆军军医大学学报(原第三军医大学学报),2006,28(14):2.
[7]黄英,刘恩梅,李渠白,等.异丙酚复合麻醉在婴幼儿纤维支气管镜术中的临床应用[J].陆军军医大学学报(原第三军医大学学报),2006,28(12):1358.
[8]孙萍.长期或间歇吸入布地奈德干预婴幼儿喘息发作的临床对照分析[J].陆军军医大学学报(原第三军医大学学报),2009,31(14):1336.
[9]王刚,吴春,潘征夏,等.2例婴幼儿先天性血管环的诊断和治疗[J].陆军军医大学学报(原第三军医大学学报),2010,32(07):668.
[10]赵利华,金先庆,梁睿,等.5例婴幼儿幽门管增生性息肉临床特征分析[J].陆军军医大学学报(原第三军医大学学报),2010,32(07):719.
 Zhao Lihua,Jin Xianqing,Liang Rui,et al.Clinical characteristics of hyperplastic pyloric canal polyp in infant and toddler: an analysis of 5 cases[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(01):719.

更新日期/Last Update: 2010-01-08