[1]韩京,冉新泽,艾国平,等.去甲肾上腺素促进骨髓间充质干细胞增殖的研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(23):2295-2298.
 HAN Jing,RAN Xin-ze,AI Guo-ping,et al.Norepinephrine improves bone marrow mesenchymal stem cells proliferation through AR/PKC-dependent signaling[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(23):2295-2298.
点击复制

去甲肾上腺素促进骨髓间充质干细胞增殖的研究(/HTML )
分享到:

陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第23期
页码:
2295-2298
栏目:
论著
出版日期:
2009-12-15

文章信息/Info

Title:
Norepinephrine improves  bone marrow mesenchymal stem cells proliferation through AR/PKC-dependent signaling
作者:
韩京冉新泽艾国平粟永萍
第三军医大学预防医学院全军复合伤研究所,创伤、烧伤与复合伤国家重点实验室
Author(s):
HAN Jing RAN Xin-ze AI Guo-ping SU Yong-ping
Sate Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Military Preventive Medicine, Third Military Medical University, Chongqing 400038, China
关键词:
骨髓间充质干细胞去甲肾上腺素肾上腺素能受体蛋白激酶C
Keywords:
bone marrow mesenchymal stem cells norepinephrine adrenergic receptors PKC
分类号:
R329.21;R329.28;R977.1
文献标志码:
A
摘要:
目的    观察去甲肾上腺素对骨髓间充质干细胞增殖的影响及其作用途径。    方法    将BMSCs细胞随机分为3组:①正常对照组;②去甲肾上腺素(10-6~10-4 mol/L)处理组;③酚妥拉明(10-6mol/L)采+去甲肾上腺素(10-5mol/L)处理组。采用3H-TdR掺入实验检测不同实验组对BMSCs增殖的影响;免疫组化和免疫印记法检测去甲肾上腺素对蛋白激酶C的作用。    结果     ①10-6~10-4 mol/L的去甲肾上腺素作用8 h后均促进了骨髓间充质干细胞的增殖,并且在10-5 mol/L 时去甲肾上腺素对骨髓间充质干细胞的促增殖作用最为显著;②酚妥拉明阻断了去甲肾上腺素对骨髓间充质干细胞的促增殖作用;③去甲肾上腺素诱导蛋白激酶C从细胞质到细胞膜转位,酚妥拉明抑制了去甲肾上腺素诱导的蛋白激酶C激活。    结论    去甲肾上腺素能够促进骨髓间充质干细胞的增殖,并且这种促增殖作用是通过肾上腺素能受体、蛋白激酶C信号通路来调节的。
Abstract:
Objective    To investigate the effects of norepinephrine on the proliferation of bone marrow mesenchymal stem cells (BMSCs) and the related signaling molecules involved in BMSCs.     Methods    3H-thymidine incorporation assay was performed to investigate the effect of norepinephrine on BMSCs proliferation,in the presence of various concentrations (10-6 to 10-4 mol/L) for 8 h or with phentolamine (10-6 mol/L) for 30 min. Effect of norepinephrine on PKC activation was detected by immunofluorescent staining and Western blot analysis.     Results    Norepinephrine treatment (10-6  to10-4 mol/L) for 8 h increased the 3H-thymidine incorporation in a dose-dependent manner. The maximal stimulatory effect was observed at 10-5 mol/L of norepinephrine. The stimulatory effect of norepinephrine on cell proliferation was blocked by co-incubation with phentolamine. Norepinephrine induced PKC translocation to the cell membrane, which was blocked by pretreatment with phentolamine for 30 min.     Conclusion    This stimulatory effect of norepinephrine on cell proliferation is mediated through AR/PKC-dependent signaling.

相似文献/References:

[1]李明,孙守兵,周越,等.去甲肾上腺素诱导人巨噬细胞MMP-9的表达及机制[J].陆军军医大学学报(原第三军医大学学报),2012,34(17):1758.
 Li Ming,Sun Shoubing,Zhou Yue,et al.Norepinephrine induces expression of matrix metalloproteinase-9 in human macrophages and its mechanisms[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(23):1758.
[2]李茂华,滕苗.严重烧伤大鼠血清对骨髓间充质干细胞迁移的影响[J].陆军军医大学学报(原第三军医大学学报),2015,37(16):1643.
 Li Maohua,Teng Miao.Serum from severely burned rats promotes migration in rat bone marrow mesenchymal stem cells through PI3K/Akt signal pathway[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(23):1643.
[3]张蕾,陈沅,田杰,等.心肌细胞介导骨髓间充质干细胞的心肌样分化[J].陆军军医大学学报(原第三军医大学学报),2005,27(16):1681.
[4]王颖楠,范雪梅,赵敏,等.脱细胞膀胱基质复合大鼠骨髓间充质干细胞体外构建组织工程化吊带治疗压力性尿失禁的初步研究[J].陆军军医大学学报(原第三军医大学学报),2012,34(22):2269.
 Wang Yingnan,Fan Xuemei,Zhao Min,et al.Preliminary study on the construction of a tissue-engineered sling with BMSCs and UBM in vitro for treating stress urinary incontinence[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(23):2269.
[5]周长立,任先军,蒋涛,等.Wnt7a基因对大鼠骨髓间充质干细胞增殖及向神经元样细胞分化的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(08):702.
 Zhou Changli,Ren Xianjun,Jiang Tao,et al.Wnt7a gene stimulates mesenchymal stem cell proliferation and differentiation into neuron-like cells[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(23):702.
[6]郝磊,邹仲敏,王军平,等.hPDGF-A/hBD2双基因共表达腺病毒载体的构建及表达[J].陆军军医大学学报(原第三军医大学学报),2007,29(10):859.
 HAO Lei,ZOU Zhong-min,WANG Jun-ping,et al.Construction and identification of recombinant adenovirus expressing hPDGF-A and hBD2[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(23):859.
[7]郭书权,吴雪晖,许建中,等.两种方法分离小型猪骨髓间充质干细胞的比较[J].陆军军医大学学报(原第三军医大学学报),2007,29(10):988.
[8]黄文秋,黄宏,徐祥,等.mTOR及其下游信号通路在骨髓间充质干细胞氧化应激损伤中的变化及作用[J].陆军军医大学学报(原第三军医大学学报),2013,35(02):114.
 Huang Wenqiu,Huang Hong,Xu Xiang,et al.Changes and roles of mTOR and its downstream signaling passway in mouse bone marrow stem cells with oxidative stress injury[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(23):114.
[9]冯一梅,徐辉,邹仲敏,等.hPDGF-A/hBD2双基因转染对大鼠骨髓间充质干细胞生物学特性的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(06):472.
 FENG Yi-mei,XU Hui,ZOU Zhong-min,et al.Effects of hPDGF-A/hBD2 genes transfection on rat bone marrow mesenchymal stem cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(23):472.
[10]姚青,宋治远,马显光.脉冲微交流电刺激促进体外诱导大鼠骨髓间充质干细胞向心肌分化[J].陆军军医大学学报(原第三军医大学学报),2008,30(05):410.
 YAO Qing,SONG Zhi-Yuan,MA Xian-guang.Electrical stimulation promotes the differentiation of rat bone marrow mesenchymal stem cells to cardiomyocyte induced by 5-azacytidine in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(23):410.

更新日期/Last Update: 2009-11-23