[1]甘露,曹川,李世荣,等.以表皮干细胞与HaCaT细胞为种子细胞构建组织工程皮肤的比较研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(20):1977-1980.
 GAN Lu,CAO Chuan,LI Shi-rong,et al.Comparison of epidermal stem cells and HaCaT cells as seed cells to construct tissue engineered skin[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(20):1977-1980.
点击复制

以表皮干细胞与HaCaT细胞为种子细胞构建组织工程皮肤的比较研究(/HTML )
分享到:

陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第20期
页码:
1977-1980
栏目:
论著
出版日期:
2009-10-30

文章信息/Info

Title:
Comparison of epidermal stem cells and HaCaT cells as seed cells to construct tissue engineered skin
作者:
甘露曹川李世荣李建福毋巨龙
第三军医大学西南医院整形外科
Author(s):
GAN Lu CAO Chuan LI Shi-rong LI Jian-fu WU Ju-long
Department of Plastic and Aesthetic Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
关键词:
表皮干细胞HaCaT细胞组织工程皮肤
Keywords:
epidermal stem cells HaCaT cells tissueengineered skin
分类号:
R318;R322.99;R329.2
文献标志码:
A
摘要:
目的   比较人表皮干细胞(epidermal stem cells, ESCs)与人永生化角质细胞HaCaT作为种子细胞构建组织工程皮肤的差异。   方法   Ⅳ型胶原快速黏附法分选表皮干细胞,无血清培养基DK-SFM培养,取第2代备用;应用DK-SFM培养HaCaT细胞,取对数生长期的细胞备用;利用组织工程技术构建真皮等价物(dermal equivalent);将上述备用的两种种子细胞分别接种在真皮替代物的表面,构建组织工程皮肤,先后进行浸没培养和气-液面器官型培养,从形态学、物理特性、HE染色等方面观察比较培养物的差异。   结果   以ESCs构建的组织工程皮肤收缩快,分层分化近似于正常皮肤,具有较好的张力和韧性。以HaCaT细胞构建的组织工程皮肤分层少,生长慢,张力韧性较差。两种皮肤的直径都随培养时间的增加而缩短,且在3~7 d各检测时相点间有显著性差异(P<0.05)。   结论   ESCs比永生化的HaCaT细胞更适合作为种子细胞构建组织工程皮肤。
Abstract:
Objective   To explore the differences of human epidermal stem cells (ESCs) and human immortalized keratinocytes HaCaT as seed cells to construct tissue engineered skin.    Methods   Human ESCs were selected by rapid attachment to collagen Ⅳ, and fed with DK-SFM. The cells at passages 2 were ready to seed. Hacat cells were also fed with DK-SFM. ESCs and Hacat cells were respectively seeded on collagen matrices populated with increasing numbers of fibroblasts and cultured for 1 week at the air liquid interface. Morphology, physical properties observation, and HE staining were carried out to compare the differences of the 2 cells.    Results   ESCs were faster in constructing a contraction of the tissue-engineered skin, and had good tension and toughness and similar stratification as normal skin. HaCaT cells were less laminated, slow growth, and had low tension and toughness in tissue-engineered skin construction. The diameter of 2 types of constructed skin samples was shortened along with the increasing of culture time, and had significant difference in 3 to 7 d.    Conclusion   Epidermal stem cells are more suitable as seed cells for tissue engineering of skin than the HaCaT immortalized cells.

相似文献/References:

[1]黄翀,江旭品,张琼,等.低氧环境对创面修复主要效应细胞表达MMP-9的作用[J].陆军军医大学学报(原第三军医大学学报),2013,35(04):293.
 Huang Chong,Jiang Xupin,Zhang Qiong,et al.Hypoxia up-regulates MMP-9 in epidermal cells during wound repair[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(20):293.
[2]阎衡,翟志芳,周春丽,等.Trappin-2对HaCaT细胞及银屑病跨膜模型增殖的抑制作用[J].陆军军医大学学报(原第三军医大学学报),2008,30(08):746.
 YAN Heng,ZHAI Zhi-fang,ZHOU Chun-li,et al.Trappin-2 inhibits the proliferation of HaCaT cells and transwell psoriatic skin organ culture models[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(20):746.
[3]刘洋,李建福,付小兵,等.皮肤软组织扩张术对大鼠表皮细胞增殖分化的影响[J].陆军军医大学学报(原第三军医大学学报),2006,28(15):1557.
[4]李金玺,刘旭盛,唐辉,等.抗生素及bFGF,EGF,rhGH对人体外培养Hacat细胞生物学特性影响[J].陆军军医大学学报(原第三军医大学学报),2006,28(14):1502.
[5]杨珂,余瑾,杨恬.表皮干细胞体外诱导转分化为角膜上皮细胞的实验研究[J].陆军军医大学学报(原第三军医大学学报),2006,28(07):671.
[6]王元元,杨桂红,杨涛,等.人原代表皮干细胞及其传代细胞生物学特性的比较[J].陆军军医大学学报(原第三军医大学学报),2011,33(01):24.
 Wang Yuanyuan,Yang Guihong,Yang Tao,et al.Comparative study on biological characteristics between human primary epidermal stem cells and their subcultured cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(20):24.
[7]崔洪勋,伍津津,唐书谦,等.表皮干细胞对Ⅳ型胶原的黏附特性及分选方法的研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(09):802.
 CUI Hong-xun,WU Jin-jin,TANG Shu-qian,et al.Sorting epidermal stem cells on basis of adhering to type Ⅳ collagen within different times[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(20):802.
[8]蔡飒,付小兵,雷永红,等.在体诱导表皮细胞去分化的初步实验研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(21):1998.
 CAI Sa,FU Xiao-bing,LEI Yong-hong,et al.Dedifferentiation of human epidermal cells in nude mice[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(20):1998.
[9]陈伟,杨恬,连小华,等.大鼠表皮干细胞的分布与体外分离培养[J].陆军军医大学学报(原第三军医大学学报),2007,29(05):376.
 CHEN Wei,YANG Tian,LIAN Xiao-hua,et al.Distribution, isolation and culture of epidermal stem cells of rats in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(20):376.
[10]唐红英,伍素华,罗向东,等.整合素β1小干扰RNA载体构建及其在表皮干细胞干扰效应检测[J].陆军军医大学学报(原第三军医大学学报),2004,26(23):0.[doi:10.16016/j.1000-5404.2004.23.015 ]
 TANG Hong-ying,WU Su-hua,LUO Xiang-dong,et al.[J].J Amry Med Univ (J Third Mil Med Univ),2004,26(20):0.[doi:10.16016/j.1000-5404.2004.23.015 ]

更新日期/Last Update: 2009-10-21