[1]叶琳,王驰,谢明均,等.粉防己碱对喉癌耐药细胞株Hep-2/ADM多药耐药逆转作用研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(12):1205-1208.
　YE Lin,WANG Chi,XIE Ming-jun,et al.Reversal effect of tetrandrine on multidrug resistance in laryngeal carcinoma[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(12):1205-1208.
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粉防己碱对喉癌耐药细胞株Hep-2/ADM多药耐药逆转作用研究(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第12期
页码:
1205-1208
栏目:
论著
出版日期:
2009-06-30

文章信息/Info

Title:
Reversal effect of tetrandrine on multidrug resistance in laryngeal carcinoma
作者:
叶琳; 王驰; 谢明均; 陈鸿雁
重庆医科大学：附属第一医院耳鼻咽喉科,药学院;宜宾市第二人民医院耳鼻咽喉科
Author(s):
YE Lin;  WANG Chi;  XIE Ming-jun;  CHEN Hong-yan
Department of Otolaryngology, First Affiliated Hospital, School of Pharmacy, Chongqing Medical University, Chongqing 400016; Second People’s Hospital of Yibing City, Yibing 644000, Sichuan Province, China
关键词:
粉防己碱; 喉癌; Hep-2/ADM细胞系; 多药耐药
Keywords:
tetrandrine;  Hep-2/ADM cell line;  multidrug resistance;  nasopharyngeal carcinoma
分类号:
R739.65;R965;R979.1
文献标志码:
A
摘要:
目的   探讨天然有效成分粉防己碱（tetrandrine，TET) 对喉癌耐药细胞株Hep-2/ADM的多药耐药逆转作用及其可能机制。   方法   采用MTT法检测不同浓度粉防己碱对Hep-2/ADM细胞的增殖抑制效应，选取其无毒剂量；MTT法检测长春新碱(vincristine，VCR)对HEP-2和Hep-2/ADM细胞增殖的抑制作用及加用粉防己碱后的变化；采用流式细胞仪检测细胞内药物蓄积和外排程度以及细胞凋亡。   结果   1.5 μg/ml及更低浓度的粉防己碱对Hep-2/ADM细胞无明显细胞毒性作用。加入1.0 μg/ml和1.5 μg/ml粉防己碱后，Hep-2/ADM对VCR的耐药逆转倍数分别为1.72和2倍。1.5 μg/ml 浓度的粉防己碱可提高VCR在Hep-2 /ADM细胞内药物的蓄积，增强VCR诱导的Hep-2/ADM细胞凋亡。   结论   粉防己碱可以部分逆转Hep-2/ADM细胞的多药耐药，随药物浓度增加，逆转效果有可能增强，其逆转机制可能与抑制P糖蛋白(P-glycoprotein，P-gp)外排功能和增强化疗药物诱导的细胞凋亡有关。
Abstract:
Objective   To study the reversal effect of tetrandrine (TET) on the multidrug resistance of Hep-2/ADM cell line and the possible mechanisms.     Methods   MTT method was used for the detection of the inhibitory effect of different concentrations of TET on the multidrug resistance of Hep-2/ADM cell line for selection of the non-toxicity concentration. MTT reduction assay was used to investigate the drug interaction of TET and vincristine (VCR) on HNE-1 (200) cell line. The reversal index (RI), accumulation and excretion of drug, and the apoptosis of Hep-2/ADM cell line were detected by flow cytometry.     Results   No toxicity to Hep-2/ADM cell line was found when TET concentration was lower than 1.5 μg/ml. After interaction with TET (1.0 μg/ml and 1.5 μg/ml), RIs of VCR on Hep-2/ADM were 1.72 and 2.00 folds, respectively. TET at the concentration of 1.5 μg/ml could increase the drug excretion and accumulation and also the apoptotic rate of VCR-induced Hep-2/ADM cell line.     Conclusion   TET can reverse the multidrug resistance of Hep-2/ADM cell line. The reversal effect may be related to the concentrations of TET. The possible mechanism may be that TET can increase the cell apoptosis of VCR-induced Hep-2/ADM cell line and can also increase the drug excretion and accumulation of the multidrug resistance cell line.

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更新日期/Last Update: 2009-05-31