[1]甘长清,王小毅.经动脉灌注新辅助化疗对局部进展期乳腺癌激素受体表达水平的上调作用[J].陆军军医大学学报(原第三军医大学学报),2009,31(18):1794-1797.
 GAN Chang-qing,WANG Xiao-yi.Transarterial infusion neoadjuvant chemotherapy up-regulates expressions of hormone receptors in local advanced breast cancer tissues[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(18):1794-1797.
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经动脉灌注新辅助化疗对局部进展期乳腺癌激素受体表达水平的上调作用(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第18期
页码:
1794-1797
栏目:
论著
出版日期:
2009-09-30

文章信息/Info

Title:
Transarterial infusion neoadjuvant chemotherapy up-regulates expressions of hormone receptors in local advanced breast cancer tissues
作者:
甘长清王小毅
重庆医科大学第一附属医院普通外科
Author(s):
GAN Chang-qing WANG Xiao-yi
Department of General Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
关键词:
乳腺肿瘤受体雌激素孕激素经动脉灌注化疗
Keywords:
breast cancer receptor estrogen progestogen transarterial infusion chemotherapy
分类号:
R730.23;R730.53;R737.9
文献标志码:
A
摘要:
目的   探讨经动脉灌注化疗对乳腺癌雌激素(estrogen receptor, ER)和孕激素受体(progesterone receptor, PR)表达的影响。   方法   应用逆转录聚合酶链式反应(RT-PCR)和免疫组化方法检测81例乳腺癌患者经动脉灌注化疗前后的雌孕激素受体表达情况。采用Western blot检测经动脉灌注化疗前后ER和PR的变化。    结果   RT-PCR结果表明,81例患者中43例(53.1%)ER mRNA 水平升高,33例(40.7%)PR mRNA 水平升高,25例(30.9%) ER 和PR mRNA 水平同时升高。免疫组织化学结果提示,35例(43.2%) ER表达水平升高,其中14例(17.3%)由阴性转为阳性;21例(26.0%)PR表达水平升高,其中10例(12.2%)由阴性转为阳性;16例(19.8%)ER和PR表达同时升高。Western blot显示,经动脉灌注化疗后,81例患者中38例(46.9%)ER 蛋白水平升高,30例(37.0%)PR蛋白水平升高,21例(25.9%)ER和PR蛋白水平同时升高。   结论   经动脉灌注化疗可有效上调ER、PR mRNA和蛋白水平的表达,有利于后续化疗药物对肿瘤细胞的杀灭。
Abstract:
Objective   To investigate the effect of transarterial infusion (TAI) chemotherapy on estrogen receptor (ER) and progesterone receptors (PR) expression in breast carcinoma tissue.    Methods   Samples of 81 patients with breast carcinoma were selected in this study. ER and PR mRNAs levels in the samples were determined by RT-PCR. Immunohistochemistry was employed to measure ER and PR expressions in different samples before and after TAI chemotherapy. Western blotting was used to assay ER and PR proteins level in the samples before and after the chemotherapy.    Results   RT-PCR results indicated that ER mRNA level was increased in 43 cases (53.1%) and PR level in 33 cases (40.7%). Meanwhile, both ER and PR mRNAs levels were increased in 25 cases (30.9%). Immunohistochemistry results showed that ER level was up-regulated in 35cases (43.2%), and PR level in 21 cases (26.0%). Both ER level and PR level were up-regulated in 16 out of 81 cases(19.8%). Western blotting also suggested that the both proteins levels of ER and PR were up-regulated in some patients after the chemotherapy.    Conclusion   Transarterial infusion neoadjuvant chemotherapy affects the mRNA and protein level of the hormone receptors, which facilitates the subsequent killing effect of chemotherapy on tumor cells. ER and PR expressions should be determined after the transarterial infusion chemotherapy, which might provide a guide for the subsequent treatment and prognosis.

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更新日期/Last Update: 2009-09-16