[1]高力,张曦,高蕾,等.不同来源基质细胞对骨髓瘤KM3细胞增殖和凋亡的影响[J].第三军医大学学报,2009,31(10):879-882.
 GAO Li,ZHANG Xi,GAO Lei,et al.Effect of stromal cells derived from different origins on proliferation and apoptosis of KM3 cells[J].J Third Mil Med Univ,2009,31(10):879-882.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第10期
页码:
879-882
栏目:
论著
出版日期:
2009-05-30

文章信息/Info

Title:
Effect of stromal cells derived from different origins on proliferation and apoptosis of KM3 cells
作者:
高力张曦高蕾陈幸华
第三军医大学新桥医院血液科
Author(s):
GAO Li ZHANG Xi GAO Lei CHEN Xing-hua
Department of Hematology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
关键词:
多发性骨髓瘤人脐血基质细胞骨髓基质细胞增殖凋亡
Keywords:
multiple myeloma human umbilical cord blood-derived stromal cells bone marrow stromal cells proliferation apoptosis
分类号:
R73-362;R730.23;R733.3
文献标志码:
A
摘要:
目的   观察人脐血基质细胞及骨髓瘤患者骨髓基质细胞对骨髓瘤KM3细胞生物学特性的作用。   方法   体外分离培养骨髓瘤骨髓基质细胞和人脐血源基质细胞,与骨髓瘤KM3细胞共培养。采用扫描电镜观察共培养后基质细胞和KM3细胞的位相关系;应用CCK-8、P I染色流式细胞仪和Annexin V/P I双标法检测不同培养条件下KM3细胞的增殖、细胞周期和凋亡率。   结果   人脐血基质细胞比骨髓瘤患者骨髓基质细胞有更强的抑制KM细胞增殖的作用;共培养后骨髓瘤患者骨髓基质细胞使KM3细胞阻滞于G0/G1期[(59.70±1.28)%],人脐血基质细胞使S期的KM3细胞比例增高[(46.07±2.46)%];KM3/MM-BMSCs组KM3细胞凋亡率为(3.26±0.12)%明显低于KM3/hUCBDSCs组KM3细胞凋亡率(4.76±0.12)%(P<0.01);KM3/MM-BMSCs组KM3细胞Bax mRNA的表达较KM3/hUCBDSCs组明显下降(P<0.01);KM3/hUCBDSCs组KM3细胞Bcl-2 mRNA的表达较KM3细胞悬浮培养组下降(P<0.05),KM3/MM-BMSCs组KM3细胞Bcl-2 mRNA的表达则明显升高,为悬浮培养组的1.982倍(P<0.01)。   结论   骨髓瘤基质细胞微环境抑制骨髓瘤细胞凋亡和死亡;而人脐血源基质细胞有更强的抑制骨髓瘤细胞增殖的作用,并促进其凋亡。
Abstract:
Objective   To observe the effect of the human umbilical cord blood-derived stromal cells (hUCBDSCs) and multiple myeloma bone marrow stromal cells (MM BMSCs) on the biological characteristics of KM3 cells.    Methods   After KM3 cells were co-cultured with hUCBDSCs or MM BMSCs, the proliferation, cell cycle and the apoptotic rate were determined by growth curve plotting and flow cytometry respectively. Real-time Q-PCR was used to measure the mRNA expressions of Bcl-2 and Bax.    Results   As compared with MM BMSCs, hUCBDSCs markedly suppressed the proliferation of the KM3 cells. KM3 cells in the KM3/MM-BMSCs co-culture group were arrested in G0/G1 phrase ,while KM3 cells in the KM3/hUCBDSCs co-culture group predominantly in S phrase. The apoptotic rate of KM3 cells in the KM3/ hUCBDSC co-culture group were higher than that in the KM3/MM-BMSCs co-culture group.    Conclusion   In comparison with MM BMSCs, hUCBDSCs inhibit the proliferation of KM3 cells and promote the apoptosis.

参考文献/References:

高力, 张曦, 高蕾, 等. 不同来源基质细胞对骨髓瘤KM3细胞增殖和凋亡的影响[J]. 第三军医大学学报,2009,31(10):879-882.

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更新日期/Last Update: 2009-05-08