[1]双卫兵,王东文,高宏飞,等.自由基在2型糖尿病大鼠膀胱逼尿肌损伤的作用与意义[J].第三军医大学学报,2009,31(19):1824-1827.
 SHUANG Wei-bing,WANG Dong-wen,GAO Hong-fei,et al.Role of free radicle in bladder detrusor impairment to T2DM rats[J].J Third Mil Med Univ,2009,31(19):1824-1827.
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自由基在2型糖尿病大鼠膀胱逼尿肌损伤的作用与意义(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第19期
页码:
1824-1827
栏目:
专题报道
出版日期:
2009-10-15

文章信息/Info

Title:
Role of free radicle in bladder detrusor impairment to T2DM rats
作者:
双卫兵王东文高宏飞原小斌
山西医科大学第一医院泌尿外科
Author(s):
SHUANG Wei-bingWANG Dong-wen GAO Hong-fei YUAN Xiao-bin
Department of Urology, First Hospital of Shanxi Medical University, Taiyuan 030001, China
关键词:
糖尿病膀胱病变自由基尿动力学大鼠
Keywords:
diabetes mellitus bladder disease free radicle urodynamics rats
分类号:
R345.4;R587.1;R694.602
文献标志码:
A
摘要:
目的   探讨自由基在糖尿病膀胱功能障碍病理生理学机制中的作用和意义。   方法   分组观测不同时间段T2DM大鼠与正常对照组大鼠膀胱逼尿肌肌条收缩实验,并将剩余膀胱组织制备匀浆,测定其中超氧化物歧化酶(SOD)的活力与丙二醛 (MDA)的含量。   结果   不同时间段T2DM组引起逼尿肌收缩的最小牵张力高于对照组。T2DM组在0~16周的收缩频率较对照组增高,在20周后减低。T2DM组最大收缩力呈现降低趋势。正常对照组膀胱匀浆中SOD第8周达最高峰后,呈现出下降趋势,至24周时有所增高。T2DM组4周时SOD出现增高,在8周和24周组大鼠膀胱中SOD较对照组显著下降。MDA在两组动物中都表现为进行性下降趋势。在第4周,T2DM组大鼠膀胱中MDA含量较对照组显著性降低。第8周出现MDA增高。T2DM组SOD/MDA的值低于正常对照组。在第4周时,T2DM组比正常对照组显著增高,第8周时,出现显著性降低。逼尿肌肌条收缩频率与逼尿肌最大收缩力之间呈负相关,(r=-0.226,P<0.05);MDA与逼尿肌最大收缩力之间呈正相关(r=0.234,P<0.01)。   结论   糖尿病可导致膀胱逼尿肌功能受损。在发病初期机体清除氧自由基的能力提高,膀胱功能处于代偿状态;随着病情进展,机体氧自由基损伤严重,膀胱逼尿肌功能失代偿。自由基损伤也是糖尿病膀胱逼尿肌损伤的重要机制之一。
Abstract:
Objective   To explore the role and significance of free radicle in the pathophsiological mechanisms of diabetic cystopathy (DCP).     Methods   The detrusor strip contraction experiments were performed and observed in the group of type 2 diabetes mellitus (T2DM) rats at different time stages and the control group. The bladder tissue samples was made into homogenate, and the activities of superoxide dismutase (SOD) and the contents of malondialdehyde (MDA) were determined.     Results   The minimum stretch forces to induce the detrusor contractions in T2DM group were larger than the control group. Compared with the control group, the detrusor contractive frequency was higher in the period of 0 to 16 weeks, but was lower after the 20th week. The maximum detrusor contractive forces in the T2DM group showed a descended tendency with the elapse of the experiment time. In the bladder homogenate of the control group, the activities of SOD was declined after reaching the peak at the stage of 8th week, and then escalated at the stage of 24th week. As for the T2DM group, the activities of SOD became higher in the 4th week, than descended at the stage of 8th and 24th weeks. The contents of MDA in both groups showed descended tendency. In T2DM group, MDA became even lower than the control group in the 4th week and became higher than the control group in the 8th week. The ratio of SOD/MDA in T2DM group was lower than the control group. In details, the ratio of SOD/MDA in T2DM group rose significantly in the 4th week, while declined in the 8th week. There was a negative correlation between the maximum detrusor contractive force and detrusor contractive frequency, and a positive correlation between the maximum detrusor contractive force and the contents of MDA.     Conclusion   The destrusor functions are impaired  by Diabetes Mellitus. In the initial stage of DCP, the bladder functions are normal, because the detrusor had a high ability to remove the free radicle. In the progression stage of DCP, the bladder functions become decompensation, because the organism is seriously injured by the free radicle. So the impairment by free radicle is one of important mechanisms of destrusor impairment of diabetic cystopathy.

参考文献/References:

双卫兵,王东文,高宏飞,等. 自由基在2型糖尿病大鼠膀胱逼尿肌损伤的作用与意义[J]. 第三军医大学学报,2009,31(19):1824-1827.

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更新日期/Last Update: 2009-09-29