[1]邹佳,李长清.脑缺血再灌注大鼠GAP-43和RhoA蛋白表达及NEP1-40对其的影响[J].第三军医大学学报,2009,31(12):1177-1180.
 ZOU Jia,LI Chang-qing.Effects of NEP1-40 on GAP-43 and RhoA expression in rats of cerebral ischemic-reperfusion model[J].J Third Mil Med Univ,2009,31(12):1177-1180.
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脑缺血再灌注大鼠GAP-43和RhoA蛋白表达及NEP1-40对其的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第12期
页码:
1177-1180
栏目:
论著
出版日期:
2009-06-30

文章信息/Info

Title:
Effects of NEP1-40 on GAP-43 and RhoA expression in rats of cerebral ischemic-reperfusion model
作者:
邹佳李长清
重庆医科大学附属第二医院神经内科
Author(s):
ZOU Jia LI Chang-qing
Department of Neurology, Second Affiliated Hospital,  Chongqing Medical University, Chongqing 400010, China
关键词:
脑缺血再灌注NEP1-40生长相关蛋白-43RhoA
Keywords:
cerebral ischemia reperfusion NEP1-40 GAP-43 RhoA
分类号:
R341;R363.27;R743.31
文献标志码:
A
摘要:
目的   观察侧脑室注射NEP1-40对脑缺血再灌注大鼠轴突再生、患肢功能恢复和RhoA信号通路活动的影响,探讨其可能的机制。   方法   将60只成年雄性SD大鼠分为假手术组、脑缺血对照组、侧脑室注射PBS组和侧脑室注射NEP1-40组;采用线栓法制作大鼠大脑中动脉缺血再灌注模型;用Western blot方法检测缺血灶周边脑组织生长相关蛋白(GAP-43)和RhoA蛋白表达;用Montoya设计的“楼梯测试”方法对患肢运动功能进行测试。   结果   ①侧脑室注射NEP1-40组大鼠缺血灶周边脑组织GAP-43在术后7 d和14 d均高于脑缺血对照组和侧脑室注射PBS组,以术后7 d最显著。②侧脑室注射NEP1-40组大鼠缺血灶周边脑组织RhoA在术后各时相点显著低于脑缺血对照组和侧脑室注射PBS组。③侧脑室注射NEP1-40组大鼠在术后各时相点“楼梯测试”结果显著高于脑缺血对照组和侧脑室注射PBS组。   结论   NEP1-40能够促进脑缺血再灌注大鼠损伤后轴突再生和运动功能恢复,其机制可能与抑制RhoA信号通路活动有关。
Abstract:
Objective   To study the effects of NEP1-40 on axon regeneration, motor function recovery of affected limbs and RhoA signal pathway in cerebral ischemia-reperfusion rats for exploring the possible mechanisms.     Methods   A total of 60 adult male Sprague-Dawley (SD) rats were equally divided into sham operation group (sham group), cerebral ischemia-reperfusion control group (control group), intra-lateral ventricle injection of PBS group (PBS group), and intra-lateral ventricle injection of NEP1-40 group (NEP1-40 group). The middle cerebral artery ischemia-reperfusion model (MCAI/R) was established by nylon monofilament occlusion method in rats. The changes in growth associated protein-43 (GAP-43) and RhoA expressions were determined by Western blotting technique. The motor function of affected limbs was tested by the “staircase test” of Montoya’s design.     Results   ① The expression of GAP-43 in NEP1-40 group was higher than that in the control and the PBS groups at 7 d and 14 d after MCAI/R, peaking at 7 d; ② The expression of RhoA in NEP1-40 group was significantly lower than that in the control and the PBS groups at each time point; ③ The results of “staircase test” in NEP1-40 group were much higher than those in the control group and the PBS groups at each time point after MCAI/R.     Conclusion   NEP1-40 can improve axon regeneration in cerebral ischemia-reperfusion rats and promote the recovery of motor function. The mechanism may be associated with the inhibition of RhoA signal pathway.

参考文献/References:

邹佳,李长清. 脑缺血再灌注大鼠GAP-43和RhoA蛋白表达及NEP1-40对其的影响[J]. 第三军医大学学报,2009,31(12):1177-1180.

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更新日期/Last Update: 2009-05-31