[1]阮艳,蹇锐,程小星,等.Bmi-1调节干性相关分子转录的初步分析[J].第三军医大学学报,2009,31(09):768-771.
 RUAN Yan,JIAN Rui,CHENG Xiao-xing,et al.Transcriptional regulatory effects of Bmi-1 on stemness-associated genes in embryonic stem cells[J].J Third Mil Med Univ,2009,31(09):768-771.
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Bmi-1调节干性相关分子转录的初步分析(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第09期
页码:
768-771
栏目:
论著
出版日期:
2009-05-15

文章信息/Info

Title:
Transcriptional regulatory effects of Bmi-1 on stemness-associated genes in embryonic stem cells
作者:
阮艳蹇锐程小星牛翰婕周恒宇郑宏庭胡福泉
第三军医大学:基础医学部微生物学教研室,重庆市微生物工程实验室,新桥医院内分泌科;解放军总医院309临床部
Author(s):
RUAN Yan JIAN Rui CHENG Xiao-xing NIU Han-jie ZHOU Heng-yu ZHENG Hong-ting HU Fu-quan
Department of Microbiology, College of Basic Medical Sciences, Third Military Medical University, Chongqing 400038; 309 Clinical Division, General Hospital of PLA, Beijing 100091; Department of Endocrinology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
关键词:
Bmi-1干细胞干性相关分子转录调控
Keywords:
Bmi-1 stem cells stemness associated genes transcriptional regulation
分类号:
R329.2;R39433;R394.3
文献标志码:
A
摘要:
目的   探讨Bmi-1对肿瘤及胚胎干细胞中干性相关分子转录表达的影响。   方法   分别构建鼠源和人源Bmi-1过表达载体pCI-GFP-mbmi1和pCI-GFP-hbmi1;瞬时转染鼠ES细胞CCE和人神经胶质瘤细胞U87;半定量RT-PCR检测胚胎干细胞中Oct-4、Nanog、c-Myc、Klf4和Sox2及肿瘤细胞中CD133、Nestin等干性分子的表达;克隆形成实验检测CCE细胞自我更新克隆形成能力。   结果   瞬时转染Bmi-1过表达载体后,CCE细胞中Nanog的表达上调,Oct-4、Sox2和Klf4的表达无明显变化;U87细胞中Nestin和Oct-4表达上调,CD133、Nanog 变化不明显;两者c-Myc的转录表达均显著降低;过表达Bmi-1促进CCE细胞自我更新克隆形成。   结论   Bmi-1参与了对肿瘤及胚胎干细胞中干性相关分子的转录调节。
Abstract:
Objective   To investigate the transcriptional regulatory effects of Bmi-1, a member of polycomb group, on stemness-associated genes in cancer and embryonic stem cells (CSCs and ESCs).    Methods   Eukaryotic expression vectors pCI-GFP-mbmi and pCI-GFP-hbmi were constructed and then transiently transfected into mouse ESCs, CCE cells and human glioma cell line, U87 cells respectively. Semi-quantitative RT-PCR was used to detect mRNA expressions of stemness factors, such as Oct-4, Nanog, c-Myc, Klf4 and Sox2 in CCE cells, and expressions of Bmi-1, Oct-4, Nanog, c-Myc, CD133 and Nestin in U87 cells. Clone formation assay was used to elucidate the potential to form self-renewal clones of CCE cells.    Results   Overexpression of Bmi-1 in CCE cells up-regulated the expression of Nanog. The expressions of other stemness factors such as Oct-4, Sox2 and Klf4 had no significant change. U87 cells transiently transfected with Bmi-1 promoted the expression of Oct-4 and Nestin. The expression level of c-Myc was reduced in the 2 kinds of cells. Overexpression of Bmi-1 promoted the formation of self-renewal clones in CCE cells.    Conclusion   Bmi-1 is involved in the transcriptional regulation of stemness-associated genes in CSCs and ESCs.

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更新日期/Last Update: 2009-04-27