[1]刘涌,周强,曹炜鹏,等.压应力对组织工程骨内人骨髓基质细胞增殖能力影响的实验研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(09):776-779.
 LIU Yong,ZHOU Qiang,CAO Wei-peng,et al.Effect of compressive stress on proliferation of human bone marrow stromal cells in tissue engineering bone[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(09):776-779.
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压应力对组织工程骨内人骨髓基质细胞增殖能力影响的实验研究(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第09期
页码:
776-779
栏目:
论著
出版日期:
2009-05-15

文章信息/Info

Title:
Effect of compressive stress on proliferation of human bone marrow stromal cells in tissue engineering bone
作者:
刘涌周强曹炜鹏郭平宋磊蒲小兵郭从涛
第三军医大学西南医院骨科,全军矫形外科中心
Author(s):
LIU Yong ZHOU Qiang CAO Wei-peng GUO Ping SONG Lei PU Xiao-bing GUO Cong-tao
Department of Orthopaedics, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
关键词:
组织工程生物反应器骨髓基质细胞压应力增殖
Keywords:
tissue engineering bone bioreactor bone marrow stromal cells compressive stress proliferation
分类号:
R318.08;R322.71;R329.28
文献标志码:
A
摘要:
目的   探讨压缩应变对组织工程骨内人骨髓基质细胞(human bone marrow stromal cells, hBMSCs)增殖的作用及适合强度范围。   方法   双相接种法构建组织工程骨,对其施加频率为0.5 Hz,压缩幅度分别为2%、5%、10%、15%、20%的压应力刺激hBMSCs(2 h/d)。倒置显微镜观察细胞形态,扫描电镜观察培育的组织工程骨,常规病理切片和HE染色观察培育组织工程骨内细胞分布及生长情况。MTT法和流式细胞技术检测细胞增殖水平及生长周期。   结果   压缩幅度为2%、5%、10%时压应力刺激可促进hBMSCs增殖;压缩幅度为15%、20%时压应力刺激抑制细胞增殖,其中以10%压缩幅度刺激细胞增殖效果最为显著,生长曲线比对照组明显前移,S期细胞百分比(27.45±0.25)%较对照组(19.15±0.26)%增高43.34%。   结论   适当强度的压应力可促进hBMSCs的增殖,5%~10%的压缩幅度是促进hBMSCs增殖的适合强度。
Abstract:
Objective   To explore the effect of compressive stress on the proliferation of the human bone marrow stromal cells (hBMSCs) seeded on demineralized bone matrix (DBM) and its optimal range of intensity.    Methods   The tissue engineered bone was built with the method of double phase inoculation after the hBMSCs were isolated and cultured from fresh bone marrow sample of healthy individual and DBM was cut into a cube of 10 mm. Compressive stress with the power to make the cell body shrink by a ratio of 2%, 5%, 10%, 15% or 20% at 0.5 Hz, was applied to the hBMSCs for 2 h per day. The cells were harvested after the stimulus for 1, 3, 5, 7, 10 and 13 d respectively. Cell morphology was observed with inverted microscopy, and the ultrastructure of the tissue engineering bone with scanning electron microscopy. Proliferation and cell cycle was measured by MTT assay and flow cytometry respectively.    Results   Compressive stress promoted hBMSCs seeded on DBM to proliferate when the compression ratio was 2%, 5% or 10%; but restrain the cells to proliferate when the compression ratios were 15% and 20%. Among all of the compression ratio, 10% showed a most prominent effect on cell proliferation. It increased the percentage of cells at the S phase from (19.15±0.26)% (in control group) to (27.45±0.25)%.    Conclusion   Compressive stress promotes the hBMSCs to proliferate in a certain range of intensity, and the intensity with the power to shrink the cell body by 5% to 10% is an optimal range to apply.

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更新日期/Last Update: 2009-04-27