[1]张志波,蔡磊,郑树国,等.Caveolin-1 RNA干扰慢病毒载体的构建及其对肝癌细胞株SMMC7721侵袭的影响[J].第三军医大学学报,2009,31(10):931-934.
 ZHANG Zhi-bo,CAI Lei,ZHENG Shu-guo,et al.Construction of recombinant lentivirol expression vector carrying human Caveolin-1 with RNA interference and its effect on invasiveness of SMMC7721 cells[J].J Third Mil Med Univ,2009,31(10):931-934.
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Caveolin-1 RNA干扰慢病毒载体的构建及其对肝癌细胞株SMMC7721侵袭的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第10期
页码:
931-934
栏目:
论著
出版日期:
2009-05-30

文章信息/Info

Title:
Construction of recombinant lentivirol expression vector carrying human Caveolin-1 with RNA interference and its effect on invasiveness of SMMC7721 cells
作者:
张志波蔡磊郑树国熊燕董家鸿
第三军医大学西南医院全军肝胆外科研究所
Author(s):
ZHANG Zhi-bo CAI Lei ZHENG Shu-guo XIONG Yan DONG Jia-hong
Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
关键词:
慢病毒Caveolin-1RNA干扰侵袭
Keywords:
lentivirus Caveolin-1 RNA interference invasiveness
分类号:
R394-33;R730.23;R735.7
文献标志码:
A
摘要:
目的   构建人Caveolin-1 RNA干扰慢病毒载体,探讨慢病毒介导的Caveolin-1 RNA干扰对人肝癌细胞株SMMC7721侵袭能力的影响。   方法   应用pGCSIL-GFP-Caveolin-1 RNA干扰慢病毒载体感染SMMC7721,检测其转导效率、mRNA和蛋白水平的敲除效率,并检测对SMMC7721侵袭能力的影响。   结果   pGCSIL-GFP-Caveolin-1 RNA干扰慢病毒载体感染SMMC7721的转导效率为(96.0±1.2)%;Caveolin-1 mRNA水平的敲除率约90%;Western blot显示感染后Caveolin-1蛋白水平显著下降;感染后SMMC7721的侵袭能力显著下降。   结论   慢病毒载体构建的Caveolin-1 RNA干扰能有效敲除SMMC7721中Caveolin-1的表达,显著降低SMMC7721侵袭能力。
Abstract:
Objective   To construct a lentivial expression vector of human Caveolin-1 with RNA interference and study its effect on the invasiveness of human hepatoma cell line SMMC7721.    Methods   After the lentiviral expression vector of human Caveolin-1 with RNA interference (pGCSIL-GFP-Caveolin-1 RNAi) was transduced into SMMC7721 cells, the transduction efficiency, the Caveolin-1 mRNA level were determined by real-time PCR, the expression of Caveolin-1 protein was measured by Western blot analysis. Its significance for the invasiveness of SMMC7721 cells were detected with the aid of Transwell.    Results   The transduction efficiency of our constructed vector pGCSIL-GFP-Caveolin-1 RNAi to SMMC7721 cells was (96.0±1.2)%. The Caveolin-1 mRNA level was knocked down by 90%. The expression of Caveolin-1 protein was significantly declined. The invasiveness of SMMC7721 cells was significantly descented.    Conclusion   A recombinant lentivirol expression vector carrying human Caveolin-1 with RNA interference is successfully constructed, and it knocks down the expression of Caveolin-1 in SMMC7721 cells efficiently and decrease the invasiveness of the cells significantly.

参考文献/References:

张志波, 蔡磊, 郑树国, 等. Caveolin-1 RNA干扰慢病毒载体的构建及其对肝癌细胞株SMMC7721侵袭的影响[J]. 第三军医大学学报,2009,31(10):931-934.

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更新日期/Last Update: 2009-05-08