[1]李兰双,胡丽娜.宫颈癌淋巴道转移模型的建立及Prox-1临床意义初探[J].第三军医大学学报,2009,31(16):1585-1588.
 LI Lan-shuang,HU Li-na.Model establishment of cervical cancer lymphatic metastasis and clinical significance of Prox-1[J].J Third Mil Med Univ,2009,31(16):1585-1588.
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宫颈癌淋巴道转移模型的建立及Prox-1临床意义初探(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第16期
页码:
1585-1588
栏目:
论著
出版日期:
2009-08-30

文章信息/Info

Title:
Model establishment of cervical cancer lymphatic metastasis and clinical significance of Prox-1
作者:
李兰双胡丽娜
重庆医科大学附属第二医院妇产科
Author(s):
LI Lan-shuang HU Li-na
Department of Obstetrics and Gynecology, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China
关键词:
宫颈癌淋巴道转移小鼠模型VEGFR-3Prox-1
Keywords:
uterine cervical cancer lymphatic metastasis mice model VEGFR-3 Prox-1
分类号:
R73-354;R730.23;R737.33
文献标志码:
A
摘要:
目的   建立小鼠宫颈癌淋巴道转移模型,初步探讨Prox-1的临床意义。   方法   将宫颈癌U27瘤株的单细胞悬液注入小鼠左爪垫,观察成瘤率、肿瘤生长及淋巴结转移情况。应用HE染色及 CK免疫组化检测各级淋巴结中有无肿瘤转移;应用荧光定量PCR和Western blot检测肿瘤组织中VEGFR-3和Prox-1 在 mRNA及蛋白水平的表达情况。   结果   爪垫种植后成瘤率100%;淋巴结转移程度与接种时间显著相关;荧光定量PCR和Western blot结果显示, 肿瘤组织中Prox-1 mRNA和蛋白在远处淋巴结转移组[(0.000 173±0.000 085),(0.108 7±0.049 9)]明显高于前哨淋巴结转移组[(0.000 058±0.000 022),(0.012 0±0.005 7)]及无淋巴结转移组[(0.000 027±0.000 010),(0.003 8±0.001 4)],3组两两比较差异有统计学意义(P<0.05),而VEGFR-3的mRNA和蛋白在前哨淋巴结组分别为(0.000 147±0.000 066) 和(0.123 6±0.054 8),与远处淋巴结转移组[(0.000 224±0.000 100),(0.160 7±0.075 3)]及无淋巴结转移组 [(0.000 134±0.000 044),(0.082 4±0.043 2)]比较均无显著性差异(P>0.05)。   结论   小鼠爪垫皮下注射可成功建立宫颈癌淋巴道转移模型;Prox-1是预测肿瘤淋巴结转移及抗肿瘤淋巴转移的敏感指标。
Abstract:
Objective   To establish the uterine cervical cancer model of lymphatic metastasis using Kunming (Km) mice and to explore the clinical significance of Prox-1.   Methods   The lymphatic metastasis model was established by injection of cervical cancer cells into the left nail pads of female Km mice. The tumor formation rate, tumor growth and lymph node metastasis status were observed. At 5, 10, 15, and 20 d after inoculation, mice in one group were sacrificed for the specimens, including lymph nodes and tumors. Then HE dyeing and immunohistochemistry staining of cytokeratin were used for the detection of the occurrence of lymphatic metastasis or not, and fluorescent quantitation polymerase chain reaction (FQ-PCR) and Western-blot were used for the detection of the expressions of VEGFR-3 and Prox-1.   Results   The tumor formation rate was 100%. The lymph node metastasis was closely correlated with the inoculation time. FQ-PCR and Western blot demon-strated that Prox-1 mRNA and protein expressions in distant lymphatic metastasis group [(0.000 173±0.000 085)  and (0.108 7±0.049 9)] were significantly higher than those in sentinel node metastasis group [(0.000 058±0.000 022) and (0.012 0±0.005 7)] and non-lymphatic metastasis group [(0.000 027±0.000 010) and (0.003 8±0.001 4)] (P<0.05). Expressions of VEGFR-3 mRNA and protein in sentinel node metastasis group were 0.000 147±0.000 066 and 0.123 6±0.054 8, respectively, but no significant difference was found as compared with those in distant lymphatic metastasis group [(0.000 224±0.000 101) and (0.160 7±0.075 3)] and non-lymphatic metastasis group [(0.000 134±0.000 044) and (0.082 4±0.043 2)] (P>0.05).   Conclusion   The model for uterine cervical cancer lymphatic metastasis can be successfully established through the nail pad hypodermic in Km mice. It is an ideal model for the studies of the mechanisms in uterine cervical metastasis as well as antitumor therapy of lymphatic transfer. Prox-1 may be a sensitive index for the prediction of tumor lymph node metastasis and antitumor lymphatic transfer.

参考文献/References:

李兰双,胡丽娜.宫颈癌淋巴道转移模型的建立及Prox-1临床意义初探[J]. 第三军医大学学报,2009,31(16):1585-1588.

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更新日期/Last Update: 2009-09-07