[1]王晓琳,邱宗荫,夏永鹏.性别差异对实验性链脲佐菌素糖尿病大鼠造模的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(17):1668-1671.
　WANG Xiao-lin,QIU Zong-yin,XIA Yong-peng.Difference in streptozotocin-induced experimental diabetic model between male and female rats[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(17):1668-1671.
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性别差异对实验性链脲佐菌素糖尿病大鼠造模的影响(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第17期
页码:
1668-1671
栏目:
论著
出版日期:
2009-09-15

文章信息/Info

Title:
Difference in streptozotocin-induced experimental diabetic model between male and female rats
作者:
王晓琳; 邱宗荫; 夏永鹏
重庆医科大学药学院;重庆市食品药品监督管理局
Author(s):
WANG Xiao-lin;  QIU Zong-yin;  XIA Yong-peng
School of Pharmacy, Chongqing Medicinal University, Chongqing 400016; Chongqing Food and Drug Administration, Chongqing 400042, China
关键词:
罗格列酮; 2型糖尿病大鼠模型; 性别; 链脲佐菌素
Keywords:
rosiglitazone;  type ; 2 Diabetic rat model;  gender;  streptozotocin
分类号:
R-332;R339.2;R587.1
文献标志码:
A
摘要:
目的   采用低剂量链脲佐菌素（streptozotocin，STZ）加高脂膳食诱导方法建立糖尿病大鼠模型，考察大鼠性别背景对造模的影响。   方法   雌性Wistar大鼠与雄性Wistar大鼠用25 mg/kg STZ加高脂膳食诱导方法分别建立糖尿病大鼠模型，并以罗格列酮作为阳性药进行验证。    结果   造模后，雌性与雄性模型大鼠均多食、多尿，血糖、血酯升高，符合糖尿病动物模型要求。与雌性模型大鼠比较，雄性大鼠造模时死亡率更高，其体质量、甘油三酯显著高于雌性大鼠（P<0.05），胰岛素水平显著低于雌性大鼠（P<0.05），胰腺病理组织观察可见雄性模型大鼠胰岛细胞的肥大增生及空泡变性均较雌性严重。罗格列酮作用4周后，能降低雌性模型大鼠的血糖水平（P<0.05），对雄性模型大鼠血糖无效。   结论   25 mg/kg STZ加高脂膳食的方法可诱导雌性Wistar大鼠建立2型糖尿病模型，但由于STZ对雌性与雄性Wistar大鼠胰岛造成的损伤程度有差异，雄性大鼠更敏感，因此雄性Wistar大鼠利用25 mg/kg STZ加膳食诱导方法不能建立2型糖尿病模型。
Abstract:
Objective   To explore the difference of male and female rats in establishing diabetic model by feeding lardy diet and intraperitoneally injecting a low dose streptozotocin (STZ).     Methods   Totally 184 male and female Wistar rats (each 92 rats) were induced to diabetes mellitus by feeding lardy diet and intraperitoneally injecting 25 mg/kg STZ for 5 weeks. For the 114 left living rats (70 females and 44 males), they were randomized into female high rosiglitazone group (n=23), male high rosiglitazone group (n=15), female low rosiglitazone group (n=23), male low rosiglitazone group (n=15), female model group (n=24), and male model group (n=14). Rosiglitazone at 2 or 0.5 mg/kg were intragastrically administered to corresponding rats once a day for 4 weeks. Another 8 health female and 8 health male rats receiving same volume solvent served as normal control. The body weight, taken food amount, fasting blood glucose, plasma insulin content and the morphology of the spleen were measured and examined  to validate the animal models.     Results   Blood glucose, total plasma lipids and cholesterol of model rats were markedly increased after STZ injection. And there were some other symptoms of model rats, such as polyuria, polydipsia and polyphagia, which indicated that diabetes had been induced in the models. The male model rats had higher mortality, body weight, triglyceride level and lower plasma insulin than in female (P<0.05). Hypertrophy and hyperplasia of the islets of Langerhans in the male pancreas were more characteristic than in that of the female. Rosiglitazone reduced fasting blood glucose in the female rats (P<0.05), but had no effect on it in the male rats.     Conclusion   Female Wistar rats are induced to develop type 2 diabetes mellitus after STZ injection and lardy diet. However, because STZ causes different damnification on the islets of Langerhans in the male and female rats, the male Wistar rats can not to be induced to have the disease by STZ and lardy diet.

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更新日期/Last Update: 2009-09-14