[1]唐春兰,杨和平,卞修武,等.应用组织芯片技术研究非小细胞肺癌中MMP-2、MMP-9的表达及临床病理意义[J].陆军军医大学学报(原第三军医大学学报),2009,31(06):518-521.
 TANG Chun-lan,YANG He-ping,BIAN Xiu-wu,et al.Clinicopathological significance of MMP-2 and MMP-9 expressions in non-small cell lung cancer: a tissue microarray study[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(06):518-521.
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应用组织芯片技术研究非小细胞肺癌中MMP-2、MMP-9的表达及临床病理意义(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第06期
页码:
518-521
栏目:
论著
出版日期:
2009-03-30

文章信息/Info

Title:
Clinicopathological significance of MMP-2 and MMP-9 expressions in non-small cell lung cancer: a tissue microarray study
作者:
唐春兰杨和平卞修武王清良章容
第三军医大学西南医院:呼吸内科,病理学研究所
Author(s):
TANG Chun-lan YANG He-ping BIAN Xiu-wu WANG Qing-liang ZHANG Rong
Department of Respiratory Diseases, Institute of Pathology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
关键词:
非小细胞肺癌组织芯片免疫组化MMP-2MMP-9
Keywords:
non-small cell lung cancer tissue microarray immunohistochemistry matrix metalloproteinases-2matrix metalloproteinases-9
分类号:
R730.23;R730.43;R734.2
文献标志码:
A
摘要:
目的    利用组织芯片技术,结合免疫组化方法,研究基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)在非小细胞肺癌(NSCLC)的表达及临床病理意义。    方法    80例NSCLC石蜡标本制成组织芯片,免疫组化(S-P)法检测 MMP-2、MMP- 9表达。    结果    MMP-2、MMP-9在NSCLC中均有较高表达率,分别为81.25%(65/80)和73.75%(59/80),而且二者表达呈高度正相关;MMP-2、MMP-9的表达在NSCLC性别、年龄、肿块大小、病理类型、分化程度上无显著差异(P>0.05);有淋巴结转移的患者MMP-2、MMP-9的阳性表达率分别为84.72%(61/72)及77.78%(56/72),无淋巴结转移的患者MMP-2、MMP-9的阳性表达率分别为50%(4/8)及37.50%(3/8)(P<0.05);临床分期为Ⅰ+Ⅱ期患者MMP-2、MMP-9的阳性表达率分别为57.14%(16/28)及53.57%(15/28),Ⅲ期患者MMP-2、MMP-9的阳性表达率分别为94.23%(49/52)及84.61%(44/52)(P<0.05)。    结论    利用组织芯片技术,结合免疫组化法可以快速、高效地检测MMP-2、MMP-9在NSCLC中的表达;MMP-2、MMP-9在NSCLC中的表达与患者性别、年龄、肿块大小、病理类型、分化程度无关,而有淋巴结转移组明显高于无淋巴结转移组,且随着临床分期增加表达明显升高; MMP-2 、MMP-9的过度表达与NSCLC的发展、淋巴结转移有密切关系,可作为临床评估NSCLC 进展的重要指标之一。
Abstract:
Objective    To explore the expressions of MMP-2 and MMP-9 in non-small cell lung cancer (NSCLC) through tissue microarray technique and immunohistochemical method and investigate the clinicopathological significance.     Methods    Totally 80 NSCLC specimens and 10 normal lung tissue samples were constructed into tissue microarray, and then immunohistochemical staining (S-P) was performed on these tissue chips. Chi-square test was employed for statistical analysis.     Results    The positive rates of MMP-2 and MMP-9 were 81.25% (65/80) and 73.75% (59/80) respectively, which had no significant difference in sex, age, size of tumor, histology and differentiation (P>0.05). The positive rates of MMP-2 and MMP-9 were significantly higher in the cases with lymphnode metastasis [84.72% (61/72) and 77.78% (56/72)] than those without lymphnode metastasis [50% (4/8) and 37.50% (3/8), P<0.05 ]. The cases  at stage Ⅲ had a positive rate of 94.23% (49/52) in MMP-2 expression and of 84.61% (44/52) in MMP-9 expression, while for those at stage  Ⅰ+Ⅱ, the rates were 57.14% (16/28) and 53.57% (15/28) with significant difference between the 2 groups. The expression of MMP-2 and MMP-9 was positively correlated.     Conclusion    The combination of tissue microarray and immunohistochemical assay is fast and effective to examine MMP-2 and MMP-9 expressions in NSCLC. The expressions of MMP-2 and MMP-9 are closely associated with lymphnode metastasis and clinical stages, but not with sex, age, size of tumor, histology and differentiation. Overexpressions of MMP-2 and MMP-9 are closely correlated to the progression, lymphnode metastasis of NSCLC, and may be used as an index for evaluation of the NSCLC progression.

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更新日期/Last Update: 2009-03-24