[1]刘晓红,曾俊伟,肖智,等.地塞米松快速抑制大鼠背根神经节细胞中ATP所致的钙升高[J].第三军医大学学报,2009,31(14):1329-1332.
 LIU Xiao-hong,ZENG Jun-wei,XIAO Zhi,et al.Dexamethasone rapidly inhibits ATP-induced intracellular calcium concentration increase in rat dorsal root ganglion neurons[J].J Third Mil Med Univ,2009,31(14):1329-1332.
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地塞米松快速抑制大鼠背根神经节细胞中ATP所致的钙升高(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第14期
页码:
1329-1332
栏目:
论著
出版日期:
2009-07-30

文章信息/Info

Title:
Dexamethasone rapidly inhibits ATP-induced intracellular calcium concentration increase in rat dorsal root ganglion neurons
作者:
刘晓红曾俊伟肖智阮怀珍
第三军医大学基础医学部神经生物学教研室,重庆市神经科学研究所;遵义医学院生理学教研室
Author(s):
LIU Xiao-hong ZENG Jun-wei XIAO Zhi RUAN Huai-zhen
Department of Neurobiology, College of Basic Medical Medicine, Third Military Medical University, Chongqing 400038; Department of Physiology, Zunyi Medical College, Zunyi 563003, Guizhou Province, China
关键词:
地塞米松背根神经节 ATP 激光共聚焦技术
Keywords:
dexamethasone dorsal root ganglion ATP single-cell Ca2+ microfluorometry techniques
分类号:
R329.27;R338.1;R977.1
文献标志码:
A
摘要:
目的   通过观察地塞米松对大鼠背根节神经元中ATP所致的细胞内游离钙离子浓度(intracellular calcium concentration,[Ca2+]i )升高的影响,了解糖皮质激素是否通过非基因组作用影响初级感觉神经元的兴奋性。   方法   分离、培养大鼠背根神经节细胞,采用激光共聚焦技术观察地塞米松对培养神经元ATP所致的[Ca2+]i升高的影响。   结果   ATP(100 μmol/L)可导致背根节神经元 [Ca2+]i升高,是由P2X受体介导细胞外Ca2+内流所致;地塞米松预孵育5 min可剂量依赖性地抑制背根节神经元ATP所致的[Ca2+]i升高,地塞米松的抑制作用可被糖皮质激素受体拮抗剂RU38486(10 μmol/L)、蛋白激酶A抑制剂H-89(10 μmol/L)所阻断。   结论   地塞米松通过糖皮质激素受体激活细胞内蛋白激酶A信号途径可显著抑制大鼠背根节神经元ATP所致的[Ca2+]i升高。糖皮质激素可通过非基因组作用影响初级感觉神经元的ATP/P2X受体功能而参与痛觉的调制。
Abstract:
Objective   To explore whether dexamethasone (Dex) has a rapid nongenomic effect on the intracellular calcium concentration ([Ca2+]i) increase after ATP inducement in rat dorsal root ganglion (DRG) neurons.    Methods   DRG neurons were isolated from SD rats at the age of 2 to 3 weeks and then plated on cover slips. The effects of Dex (1 μmol/L) on ATP-induced [Ca2+]i increase were studied on cultured DRG neurons using single-cell Ca2+ microfluorometry, respectively combined with G-protein activation inhibitor GDP-β-S (0.5 mmol/L), glucocorticoid receptor antagonist RU38486 (10 μmol/L), protein kinase A inhibitor H89 (10 μmol/L), or protein kinase C inhibitor chelerythrine chloride (Che, 10 μmol/L).    Results   ATP (100 μmol/L) caused the increase of [Ca2+]i transients in DRG neurons via activation of P2X receptor. A 5 min incubation with Dex inhibited ATP-induced [Ca2+]i increase in DRG neurons in a concentration-dependent manner. Dex inhibitory effect on ATP-induced [Ca2+]i transients were blocked by RU38486 and H89, but not GDP-β-S and Che.    Conclusion   Dex can inhibit ATP-induced [Ca2+]i increase in DRG neurons. The inhibitory effect of Dex may be mediated by glucocorticoid receptor through activating protein kinase A signal pathway. Our results demonstrate an nongenomic effect of Dex on the excitability of dorsal root ganglion neurons by regulating ATP-induced [Ca2+]i transients, which may be relevant for relieving pain in the stressful event.

参考文献/References:

刘晓红, 曾俊伟, 肖智, 等. 地塞米松快速抑制大鼠背根神经节细胞中ATP所致的钙升高[J]. 第三军医大学学报,2009,31(14):1329-1332.

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更新日期/Last Update: 2009-06-29