[1]陈方祥,滕方,廖自福,等.非控制失血性休克大鼠输液输血的研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(11):1029-1031.
 CHEN Fang-xiang,TENG Fang,LIAO Zi-fu,et al.Resuscitation for hemorrhagic shock rats: lactated Ringer’s solution with whole blood or red blood cells[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(11):1029-1031.
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非控制失血性休克大鼠输液输血的研究(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第11期
页码:
1029-1031
栏目:
论著
出版日期:
2009-06-15

文章信息/Info

Title:
Resuscitation for hemorrhagic shock rats: lactated Ringer’s solution with whole blood or red blood cells
作者:
陈方祥 滕方 廖自福刘良明胡波
第三军医大学大坪医院野战外科研究所:输血科,第二研究室,创伤、烧伤与复合伤国家重点实验室,检验科
Author(s):
CHEN Fang-xiang TENG Fang LIAO Zi-fu LIU Liang-ming HU Bo
Department of Blood Transfusion,  State Key Laboratory of Trauma, Burns and Combined Injury, Department 2, Department of Laboratory Medicine, Institute of Surgery Research,  Daping Hospital, Third Military Medical University, Chongqing 400042, China
关键词:
大鼠失血性休克羟乙基淀粉全血红细胞
Keywords:
rat hemorrhagic shock hydroxyethyl starch whole blood red blood cells
分类号:
R457;R605.971;R977.8
文献标志码:
A
摘要:
目的   观察非控制失血性休克大鼠输注不同血液成分的复苏效果。   方法   SD大鼠24只,复制非控制失血性休克(失血45%)模型。用随机数字表法将大鼠分为3组,每组8只,Ⅰ组输注林格液+羟乙基淀粉(LR+HES),Ⅱ组输注LR+HES+自身全血,Ⅲ组输注LR+HES+自体红细胞。观察休克前、休克1 h、复苏2 h各时间点血常规、心率(HR)、血压、左心室收缩压(LVSP)、左心室压力上升和下降的最大变化速率(±dp/dtmax)和存活时间。   结果   血常规指标各组休克1 h、复苏2 h与休克前比较均显著下降(P<0.01),Ⅱ、Ⅲ组与Ⅰ组比较差异有统计学意义(P<0.01),各组复苏2 h与休克1 h比较差异有统计学意义(P<0.01);存活时间Ⅱ、Ⅲ组显著长于Ⅰ组(P<0.01);HR、LVSP和±dp/dtmax 各组休克1 h、复苏2 h与休克前比较显著降低(P<0.01),Ⅱ、Ⅲ组与Ⅰ组比较差异有统计学意义(P<0.01,P<0.05),各组复苏2 h与休克1 h比较差异有统计学意义(P<0.01)。   结论   大鼠急性失血45%用晶体液和胶体液补充血容量,输注自身红细胞纠正贫血可以有效复苏,没有发生凝血异常表现。
Abstract:
Objective   To compare the resuscitation effect of lactated Ringer’s solution (LR) combined with hydroxyethyl starch (HES), whole blood or red blood cells on hemorrhagic shock rats.    Methods   SD rats (n=24) were inflicted with a sharp resection of 75% of the tail to initiate the hemorrhage. When the blood pressure (BP) was decreased to 40 mmHg, LR solution was infused to the rats for 1 h. Then the animals were randomly divided into 3 groups, LR+HES (group Ⅰ), LR+HES+auto-whole blood (group  Ⅱ), and LR+HES+auto-red blood cells groups (group   Ⅲ), and received the corresponding infusion respectively for 2 h when the BP was increased to 80 mmHg. The blood samples were collected before shock, 1 h after shock and 2 h after resuscitation. Hematologic and hemodynamics parameters, such as blood cell count, heamoglobin, hematocrit, platelet count, heart rate, BP, survival time, left intraventricular systolic pressure (LVSP), maximal change rate of left intraventricular pressure (±dp/dtmax), were measured and recorded.    Results   Hematologic parameters of each group had significant difference in 1 h after shock compared with 2 h after resuscitation, and the 2 time points compared with before shock. Those of group  Ⅱ and group   Ⅲ   were significantly higher than those of group  Ⅰ. In every group, the parameters at the time of 2 h after resuscitation had significant difference to those at 1 h after shock. Group Ⅱ and group   Ⅲ    had a significantly longer survival time compared with group Ⅰ. HR, LVSP and ±dp/dtmax  in each group at 1 h after shock and 2 h after resuscitation were significantly decreased compared with before shock. Those of group Ⅱ and group    Ⅲ   were significantly higher compared with group   Ⅰ; those of each group in 2 h after resuscitation were markedly increased compared with 1 h after shock.    Conclusion   LR solution plus HES has efficient effect on the resuscitation of hemorrhagic shock rats to maintain normovolemia, and the infusion of auto-red blood cells is helpful to correct anemia. Plasma-poor red cell concentrates is safe and causes no abnormal phenomena of blood clotting.

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更新日期/Last Update: 2009-05-21