[1]杜晓兰,陈志,尹良军,等.欧几里德几何距离矩阵法对FGFR2 Ser252Trp点突变小鼠头颅形状特征的分析[J].第三军医大学学报,2009,31(08):655-658.
 DU Xiao-lan,CHEN Zhi,YIN Liang-jun,et al.Skull morphology of mice carrying gain-of-function mutation of FGFR2 Ser252Trp by Euclidean distance matrix analysis[J].J Third Mil Med Univ,2009,31(08):655-658.
点击复制

欧几里德几何距离矩阵法对FGFR2 Ser252Trp点突变小鼠头颅形状特征的分析(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第08期
页码:
655-658
栏目:
论著
出版日期:
2009-04-30

文章信息/Info

Title:
Skull morphology of mice carrying gain-of-function mutation of FGFR2 Ser252Trp by Euclidean distance matrix analysis
作者:
杜晓兰陈志尹良军何启芬段亚琪陈林
第三军医大学大坪医院野战外科研究所全军战创伤中心,创伤、烧伤与复合伤国家重点实验室;四川省绵阳市疾病预防控制中心微生物所
Author(s):
DU Xiao-lan CHEN Zhi YIN Liang-jun HE Qi-fen DUAN Ya-qi CHEN Lin
State Key Laboratory of Trauma, Burns and Combined Injury, Trauma Center, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042;Institute of Microorganism, Centre of Disease Control and Prevention of Mianyang City, Mianyang 621000, Sichuan Province, China
关键词:
2型成纤维细胞生长因子受体小鼠模型Apert综合征头颅形态欧几里德几何距离矩阵法
Keywords:
fibroblast growth factor receptor 2 mouse model Apert syndrome skull morphology Euclidean distance matrix analysis
分类号:
R311;R323.1
文献标志码:
A
摘要:
目的    通过定量分析模拟Apert综合征患者的FGFR2 Ser252Trp突变小鼠模型和野生型小鼠头颅表型,并与Apert患者所表现出来的异常头颅颜面特征进行比较,探讨所建立的Apert小鼠模型能否真实地模拟Apert综合征患者的头颅表型。    方法    采用蚂蚁啃食的方法制备Ser252Trp突变小鼠和同窝对照小鼠头颅标本,用三维坐标测量仪检测头颅27个标记点的三维坐标值,用欧几里德几何距离矩阵法(euclidean distance matrix analysis, EDMA)对突变小鼠和对照小鼠头颅三维数据进行处理和统计分析。    结果    Ser252Trp突变小鼠头颅形状与同窝对照组相比具有统计学差异(P<0.01),突变小鼠头颅长度缩短,且头颅前端缩短比后部缩短更严重,头颅面部鼻骨长度、前颌骨、上颌骨及颧骨长度明显缩短(P<0.01),眼间距和额骨宽度变宽,颅腔长度有所缩短,宽度增加,颅顶升高(P<0.01),这与Apert患者颅面的临床表现高度相似:眼间距增宽,面中部发育不良,鼻骨缩短,上颌骨发育低下,突眼,颅腔变宽呈圆鼓状。    结论    FGFR2 Ser252Trp突变小鼠从表型上真实地反映Apert患者的头颅颜面特征,可以作为人类Apert综合征的较好模型用于有关疾病致病机制、手术及药物治疗的研究模型。
Abstract:
Objective    To validate the mouse carrying Ser252Trp mutation in fibroblast growth factor receptor (FGFR2) as a model for human Apert syndrome resulting from equivalent mutation by quantitatively analyzing the morphologic changes of mouse skull.     Methods    Euclidean distance matrix analysis (EDMA) was used to quantitatively compare the morphologic difference between the skulls of mutants and their wild type controls by analyzing the 3D coordinate data of skulls’27 landmarks collected by a 3D coordinate Measuring Machine.     Results    There was significant differences in substantial morphology between skulls of mutants and their matched controls (P<0.01). The mutants showed shortened length along anterior-posterior axis, especially in the anterior portion of skulls. The mutants exhibited significantly decreased lengths in the nasal bone, premaxilla, maxilla and zygoma (P<0.01), as well as broadened interorbital and frontal bone width (P<0.01). All these anomalies subsequently led to shortened A-P length, as well as the increased width and height in mutant skulls, which highly similar to those skull features of humans with Apert syndrome such as brachycephaly, midface dysplasia, widened distance between eyes, shortened nasal bones, and expanded cranial volumes, etc.     Conclusion    The FGFR2 Ser252Trp mutation shows the characteristic skull phenotypes that resemble those of human with Apert syndrome. Our results indicate that this mouse strain can be used as a good model for human Apert syndrome.

参考文献/References:

杜晓兰,陈志,尹良军,等. 欧几里德几何距离矩阵法对FGFR2 Ser252Trp点突变小鼠头颅形状特征的分析[J]. 第三军医大学学报,2009,31(8):655-658.

相似文献/References:

[1]李兰双,胡丽娜.宫颈癌淋巴道转移模型的建立及Prox-1临床意义初探[J].第三军医大学学报,2009,31(16):1585.
 LI Lan-shuang,HU Li-na.Model establishment of cervical cancer lymphatic metastasis and clinical significance of Prox-1[J].J Third Mil Med Univ,2009,31(08):1585.
[2]胡琦,钟世民,汪金玉,等.皮下注射大剂量卵白蛋白诱导小鼠哮喘免疫耐受模型的建立及机制初步研究[J].第三军医大学学报,2016,38(12):1404.
 Hu Qi,Zhong Shimin,Wang Jinyu,et al.Establishment of mouse model of immune tolerance of asthma through subcutaneous injection of high dose of ovalbumin[J].J Third Mil Med Univ,2016,38(08):1404.
[3]杨子峰,杨春光,王玉涛,等.从乙型流感病毒流行株中筛选获得鼠肺适应株的研究[J].第三军医大学学报,2014,36(05):446.
 Yang Zifeng,Yang Chunguang,Wang Yutao,et al.Screening of mouse-lung adapted strain from clinical influenza B viruses[J].J Third Mil Med Univ,2014,36(08):446.
[4]范文婷,钟世民,胡琦,等.色氨酸代谢物调控Th17/Treg分化在小鼠哮喘变应原特异性免疫治疗中的作用及机制研究[J].第三军医大学学报,2018,40(08):658.
 FAN Wenting,ZHONG Shimin,HU Qi,et al.Tryptophan metabolites regulate Th17/Treg differentiation to alleviate airway inflammation in asthmatic mice receiving allergen-specific immunotherapy[J].J Third Mil Med Univ,2018,40(08):658.
[5]余静,唐婉琦,杨雪,等.Musculin KO-Foxp3-GFPKI小鼠模型的制备及在严重创伤介导Treg-Th17失衡中的初步应用[J].第三军医大学学报,2018,40(16):1435.
 YU Jing,TANG Wanqi,YANG Xue,et al.Establishment of a transgenic mouse model with musculin knockout and Foxp3-GFP knockin and role of musculin in severe trauma-induced Treg-Th17 imbalance[J].J Third Mil Med Univ,2018,40(08):1435.
[6]杜晓兰,陈志,尹良军,等.Apert综合征模型小鼠下颌骨形态特点的定量分析[J].第三军医大学学报,2009,31(17):1625.
 DU Xiao-lan,CHEN Zhi,YIN Liang-jun,et al.Quantitative analysis on developmental morphologic changes on mandibles of Apert syndrome mice[J].J Third Mil Med Univ,2009,31(08):1625.

更新日期/Last Update: 2009-04-15