[1]毛成刚,郭玥馨,罗庆,等.Livinα和Livinβ在儿童急性白血病骨髓单个核细胞中的表达及临床意义[J].陆军军医大学学报(原第三军医大学学报),2009,31(15):1488-1490.
 MAO Cheng-gang,GUO Yue-xin,LUO Qing,et al.Expressions of Livin α and Livin β in marrow mononuclear cells of childhood acute leukemia and the clinical significance[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(15):1488-1490.
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Livinα和Livinβ在儿童急性白血病骨髓单个核细胞中的表达及临床意义(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第15期
页码:
1488-1490
栏目:
论著
出版日期:
2009-08-15

文章信息/Info

Title:
Expressions of Livin α and Livin β in marrow mononuclear cells of childhood acute leukemia and the clinical significance
作者:
毛成刚郭玥馨罗庆徐酉华
重庆医科大学附属儿童医院:血液科,儿科研究所
Author(s):
MAO Cheng-gang GUO Yue-xin LUO Qing XU You-hua
Department of Hematology, Institute of Pediatrics, Children’s Hospital, Chongqing Medical University, Chongqing 400014, China
关键词:
Livin凋亡抑制蛋白急性白血病儿童
Keywords:
LivinIAPacute leukemiachildren
分类号:
R394.3;R730.43;R733.71
文献标志码:
A
摘要:
目的   研究Livinα和Livinβ在儿童急性白血病(childhood acute leukemia,CAL)骨髓单个核细胞(marrow mononuclear cell,MMNC)中的表达与临床意义。   方法   采用实时PCR(real-time PCR)和Western blot检测Livinα和Livinβ在CAL骨髓单个核细胞中mRNA水平和蛋白质水平的表达。   结果   Livinα和Livinβ在初诊的39例儿童急性淋巴细胞白血病(acute lymphoblastic leukemia,ALL)和12例急性髓细胞白血病(acute myeloid leukemia,AML)MMNC中的表达率和表达水平均高于对照组(P<0.05),Livinα的表达水平高于Livinβ(P<0.01)。ALL化疗后第19天,幼稚细胞≤5%组Livinα和Livinβ的表达水平较化疗前明显降低(P<0.01)。幼稚细胞>5%组化疗后较化疗前也降低(P<0.05),但化疗后Livinα和Livinβ的表达水平仍明显高于对照组(P<0.01)。    结论   Livinα和Livinβ在CAL中高表达可能对评估CAL临床危险度及预后有重要意义;提示Livinα和Livinβ可作为观察CAL预后的分子指标之一。
Abstract:
Objective   To investigate the expressions of Livin α and Livin β in marrow mononuclear cells (MMNCs) of childhood acute leukemia (CAL) and explore the clinical significance.   Methods   Real-time quantitative PCR and Western blot were used to detect the mRNA and protein expressions of Livin α and Livin β in MMNCs of CAL, respectively.   Results   Both Livinα and Livinβ expression rates and expression levels were higher in preliminary diagnosis group(n=51)of CAL ,including acute lymphoblastic leukemia(n=39)and acute myeloid leukemia (n=12), compared with the control group(P<0.05), and the expression level of Livinα was higher than Livinβ(P<0.01). The expression levels of Livinα and Livinβ were significantly lower in patients with a favorable day 19 bone marrow response (leukemic blasts ≤5%) to induction chemotherapy of ALL than in the patients without chemotherapy(P<0.01). Though the expression levels of Livinα and Livinβ were also lower in patients with a unfavorable day 19 bone marrow response (leukemic blasts>5%) to induction chemotherapy of ALL than in the patients without chemotherapy(P<0.05), the expression levels of Livinα and Livinβ were still significantly higher than that of the control group(P<0.01).    Conclusion   The expression of Livinα and Livinβ may be associated with the genesis and development of CAL. It seems that the expression of Livinα and Livinβ may be used as a molecular marker of CAL.

相似文献/References:

[1]刘川,吴小候,张唯力,等.Livin反义寡核苷酸诱导人肾癌细胞凋亡及其机制的研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(17):1622.
 LIU Chuan,WU Xiao-hou,ZHANG Wei-li,et al.Antisense oligonucleotide targeting livin induces apoptosis of human renal carcinoma cell 786-O in vitro and its mechanism[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(15):1622.
[2]肖虹,姬长友,陈继川.凋亡抑制因子Livin在喉鳞癌中的表达及临床意义[J].陆军军医大学学报(原第三军医大学学报),2006,28(11):1226.
[3]李凡,王小毅,陈力,等.Livin靶向RNA干扰对MDA-MB-231细胞增殖和凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(24):2302.
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[4]孙建国,陈正堂,王志新,等.凋亡抑制蛋白Livin两种异构体真核表达载体的构建及表达鉴定[J].陆军军医大学学报(原第三军医大学学报),2005,27(19):1935.

更新日期/Last Update: 2009-07-23