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Livinα和Livinβ在儿童急性白血病骨髓单个核细胞中的表达及临床意义(/HTML )

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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

Title:: Expressions of Livin α and Livin β in marrow mononuclear cells of childhood acute leukemia and the clinical significance

Author(s):: MAO Cheng-gang; GUO Yue-xin; LUO Qing; XU You-hua; Department of Hematology, Institute of Pediatrics, Children’s Hospital, Chongqing Medical University, Chongqing 400014, China

摘要:: 目的研究Livinα和Livinβ在儿童急性白血病（childhood acute leukemia，CAL）骨髓单个核细胞（marrow mononuclear cell，MMNC）中的表达与临床意义。方法采用实时PCR（real-time PCR）和Western blot检测Livinα和Livinβ在CAL骨髓单个核细胞中mRNA水平和蛋白质水平的表达。结果 Livinα和Livinβ在初诊的39例儿童急性淋巴细胞白血病（acute lymphoblastic leukemia，ALL）和12例急性髓细胞白血病（acute myeloid leukemia，AML）MMNC中的表达率和表达水平均高于对照组（P<0.05），Livinα的表达水平高于Livinβ（P<0.01）。ALL化疗后第19天，幼稚细胞≤5%组Livinα和Livinβ的表达水平较化疗前明显降低（P<0.01）。幼稚细胞＞5%组化疗后较化疗前也降低（P<0.05），但化疗后Livinα和Livinβ的表达水平仍明显高于对照组（P<0.01）。结论 Livinα和Livinβ在CAL中高表达可能对评估CAL临床危险度及预后有重要意义；提示Livinα和Livinβ可作为观察CAL预后的分子指标之一。

Abstract:: Objective To investigate the expressions of Livin α and Livin β in marrow mononuclear cells (MMNCs) of childhood acute leukemia (CAL) and explore the clinical significance. Methods Real-time quantitative PCR and Western blot were used to detect the mRNA and protein expressions of Livin α and Livin β in MMNCs of CAL, respectively. Results Both Livinα and Livinβ expression rates and expression levels were higher in preliminary diagnosis group（n=51）of CAL ，including acute lymphoblastic leukemia（n=39）and acute myeloid leukemia （n=12), compared with the control group（P<0.05）, and the expression level of Livinα was higher than Livinβ（P<0.01）. The expression levels of Livinα and Livinβ were significantly lower in patients with a favorable day 19 bone marrow response (leukemic blasts ≤5%) to induction chemotherapy of ALL than in the patients without chemotherapy（P<0.01）. Though the expression levels of Livinα and Livinβ were also lower in patients with a unfavorable day 19 bone marrow response (leukemic blasts＞5%) to induction chemotherapy of ALL than in the patients without chemotherapy（P<0.05）, the expression levels of Livinα and Livinβ were still significantly higher than that of the control group（P<0.01）. Conclusion The expression of Livinα and Livinβ may be associated with the genesis and development of CAL. It seems that the expression of Livinα and Livinβ may be used as a molecular marker of CAL.

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更新日期/Last Update: 2009-07-23