[1]沈伟伟,赵建华.脉冲电磁场对废用性骨质疏松大鼠骨密度和血清转化生长因子β1的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(10):935-937.
 SHEN Wei-wei,ZHAO Jian-hua.Effects of pulsed electromagnetic fields on BMD and TGF-β1 in disuse osteoporosis rats[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(10):935-937.
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脉冲电磁场对废用性骨质疏松大鼠骨密度和血清转化生长因子β1的影响(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第10期
页码:
935-937
栏目:
论著
出版日期:
2009-05-30

文章信息/Info

Title:
Effects of pulsed electromagnetic fields on BMD and TGF-β1 in disuse osteoporosis rats
作者:
沈伟伟赵建华
第三军医大学大坪医院野战外科研究所骨科
Author(s):
SHEN Wei-wei ZHAO Jian-hua
Department of Orthopaedics, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China
关键词:
脉冲电磁场废用性骨质疏松骨密度转化生长因子β1
Keywords:
pulsed electromagnetic fields disuse osteoporosis bone mineral density transforming growth factor-beta 1
分类号:
R454.1;R681.102
文献标志码:
A
摘要:
目的   观察脉冲电磁场(pulsed electromagnetic fields, PEMF)对废用性骨质疏松(disuse osteoporosis, DOP)大鼠骨密度(bone mineral density, BMD)和血清转化生长因子β1(transforming growth factor-beta 1, TGF-β1)的影响,探讨可能的发生机制。   方法   健康4月龄雌性SD大鼠80只,按体质量随机区组法分成4组:正常对照组(INT组)、单纯模型组(DOP组)、降钙素组(CT组)、脉冲电磁场组(PEMF组)。INT组大鼠喂养DOP大鼠饲料消耗量的平均水平,后3组均通过改良的局部制动法建立DOP大鼠模型;CT组和PEMF组术后即分别给予降钙素(2 IU/kg,腹腔注射,1次/d)及PEMF预防性治疗,各组分别于建模后1、2、4、8周时处死5只大鼠,进行骨密度、骨组织学和血清TGF-β1浓度等指标检测。   结果   建模后2周时,DOP组大鼠制动侧股骨近端骨密度较INT组明显降低(P<0.01),从建模后4周起,CT组和PEMF组骨密度均明显高于DOP组(P<0.05),8周时PEMF组骨密度较CT组明显增高(P<0.05);DOP组大鼠血清TGF-β1浓度在建模后1周时明显高于INT组(P<0.01),而后逐渐下降至正常水平以下;8周时PEMF组TGF-β1浓度明显高于CT组、DOP组(P<0.01)。   结论   PEMF及降钙素对废用性骨质疏松均有治疗作用,且PEMF在后期较降钙素效果更明显;TGF-β1 浓度在废用性骨质疏松骨重建过程中呈双相变化,PEMF可能通过促进TGF-β1分泌而影响骨重建过程。
Abstract:
Objective   To investigate the effects of pulsed electromagnetic fields (PEMF) on the bone mineral density (BMD) and serum level of transforming growth factor-beta 1 (TGF-β1) in the disuse osteoporosis (DOP) rats.    Methods   Eighty 4-month-old female SD rats were randomly divided into 4 matched groups according to their weight: control group (INT group), DOP group, calcitonin group (CT group) and PEMF group. Rats in INT group were raised with the average amount of food consumed by the DOP rats. Right hindlimbs of rats in DOP, CT and PEMF groups were immobilized by tibia-tail fixation to establish DOP model as Zarzhevsky described . Rats in CT group were injected with calcitonin (2 IU/kg, i.p, once a day), and rats in group PEMF were irradiated with PEMF (8 Gs, 15 Hz, a pulse time of 8 ms) immediately after operation. The rats (5 animals per time of each group) were killed at 1, 2, 4 and 8 weeks after operation and their right hindlimbs were resected. The BMD and serum TGF-β1concentration were measured with a dual energy X-ray bone densitometer and ELISA respectively.    Results   Significant difference was observed in the femur proximal BMD between rats with right hindlimb immobilization (DOP group) and INT group 2 weeks later (P<0.01). Compared with CT group and DOP group, the BMD and serum TGF-β1concentration in PEMF group were increased significantly at 8 weeks after operation (P<0.05).    Conclusion   PEMF and calcitonin can prevent BMD decline in DOP rats, but PEMF is more effective at later stage. There is a diphase change of TGF-β1in DOP rats, PEMF may affect bone remodeling by promoting TGF-β1secretion.

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更新日期/Last Update: 2009-05-08