[1]苟巧,米粲,胡迎春,等.Pten基因对小鼠胚胎成纤维细胞中Cu/Zn-SOD基因表达的调控[J].第三军医大学学报,2009,31(12):1127-1130.
 GOU Qiao,MI Can,HU Ying-chun,et al.Pten gene regulates the expression of Cu/Zn superoxide dismutase gene in mouse embryonic fibroblasts[J].J Third Mil Med Univ,2009,31(12):1127-1130.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第12期
页码:
1127-1130
栏目:
论著
出版日期:
2009-06-30

文章信息/Info

Title:
Pten gene regulates the expression of Cu/Zn superoxide dismutase gene in mouse embryonic fibroblasts
作者:
苟巧米粲胡迎春李刚吴德昌霍艳英
重庆医科大学基础医学院病理学教研室;军事医学科学院放射与辐射医学研究所
Author(s):
GOU Qiao MI Can HU Ying-chun LI Gang WU De-chang HUO Yan-ying
Department of Pathology, College of Basic Medical Sciences Chongqing Medical University, Chongqing 400016; Institute of Radiation Medicine, Academy of Military Medicine, Beijing 100850, China
关键词:
Pten超氧化物歧化酶基因表达AKT信号通路
Keywords:
Pten superoxide dismutase gene expression AKT signal pathway
分类号:
R321;R394-33;R394.3
文献标志码:
A
摘要:
目的   研究Pten基因对抗氧化蛋白Cu/Zn-SOD基因表达的调控,初步探索其调控机制。   方法     运用Northern blot比较Pten+/+MEFs与Pten-/- MEFs细胞中基础Cu/Zn-SOD mRNA表达差异,以及0.1 mmol/L H2O2诱导后Pten+/+MEFs与Pten-/- MEFs细胞中Cu/Zn-SOD mRNA表达差异;运用Western blot分析PI3K/AKT通路抑制剂 LY294002作用Pten-/- MEFs细胞不同时间(30 min,1、2、6、24 h)后,与空白Pten-/- MEFs细胞相比,磷酸化AKT及Cu/Zn-SOD蛋白表达变化;利用Northern blot分析LY294002作用Pten-/- MEFs细胞30 min,1、2、6、24 h后,与空白Pten-/- MEFs细胞相比,Cu/Zn-SOD mRNA表达变化。   结果   Pten-/- MEFs细胞中,基础Cu/Zn-SOD mRNA表达水平降低,H2O2诱导的Cu/Zn-SOD mRNA表达明显受到抑制;在LY294002作用Pten-/- MEFs细胞30 min时,AKT磷酸化水平明显降低,2 h时基本没有表达;与此对应,在LY294002作用Pten-/- MEFs细胞30 min时,Cu/Zn-SOD蛋白及mRNA表达均增强,并持续增强至24 h。   结论   小鼠胚胎成纤维细胞中,Pten基因可通过拮抗PI3K/AKT信号通路调控Cu/Zn-SOD蛋白及mRNA 的表达。
Abstract:
Objective   To study the regulatory effect of phosphatase and tensin homolog deleted on chromosome ten (Pten) gene on the expression of Cu/Zn superoxide dismutase (Cu/Zn-SOD) gene and to explore the possible mechanisms in mouse embryonic fibroblasts.     Methods   Northern blot was employed to measure the basal level of Cu/Zn-SOD mRNA and the expression of Cu/Zn-SOD mRNA after treatment with 0.1 mmol/L H2O2 in Pten+/+ and Pten-/- MEFs. Western blot was used to analyze the protein levels of phospho-AKT and Cu/Zn-SOD in Pten-/- MEFs treated with 30 μmol/L LY294002 for 0, 0.5, 1, 2, 6, and 24 h. Northern blot was applied to examine the mRNA level of Cu/Zn-SOD in Pten-/- MEFs treated with 30 μmol/L LY294002 for 0, 0.5, 1, 2, 6, and 24 h.     Results   In Pten-/- MEFs, the basal level of Cu/Zn-SOD mRNA decreased; the 0. mmol/L H2O2-induced expression of Cu/Zn-SOD mRNA was inhibited, and the protein level of phospho-AKT decreased at 0.5, 1, 2, 6, and 24 h after treatment with 30 μmol/L LY294002. Correspondingly, the protein and mRNA levels of Cu/Zn-SOD increased at 0.5, 1, 2, 6, and 24 h after treatment with 30 μmol/L LY294002.     Conclusion   Pten gene may regulate the expression of Cu/Zn-SOD gene through the inhibition of the PI3K/AKT signal pathway in mouse embryonic fibroblasts.

参考文献/References:

苟巧,米粲,胡迎春,等. Pten基因对小鼠胚胎成纤维细胞中Cu/Zn-SOD基因表达的调控[J]. 第三军医大学学报,2009,31(12):1127-1130.

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更新日期/Last Update: 2009-05-31