[1]段东,李少林,朱玉泉,等.99Tcm-EGFR-McAb及99Tcm-CD44-McAb单独及联合用于荷人肺腺癌裸鼠的显像研究[J].第三军医大学学报,2009,31(13):1287-1290.
 DUAN Dong,LI Shao-lin,ZHU Yu-quan,et al.Effects of radioimmunoimaging with 99Tcm-EGFR-McAb or 99Tcm-CD44-McAb or combined application of both on nude mice bearing human lung adenocarcinoma[J].J Third Mil Med Univ,2009,31(13):1287-1290.
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99Tc-EGFR-McAb及99Tc-CD44-McAb单独及联合用于荷人肺腺癌裸鼠的显像研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第13期
页码:
1287-1290
栏目:
论著
出版日期:
2009-07-15

文章信息/Info

Title:
Effects of radioimmunoimaging with  99Tcm-EGFR-McAb or  99Tcm-CD44-McAb or combined application of both on nude mice bearing human lung adenocarcinoma
作者:
段东李少林朱玉泉王树斌
重庆医科大学附属第一医院核医学科,重庆医科大学基础医学院核医学教研室
Author(s):
DUAN Dong LI Shao-lin ZHU Yu-quan WANG Shu-bin
Department of Nuclear Medicine,  First Affiliated Hospital; Department of Nuclear Medicine, College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China
关键词:
肿瘤表皮生长因子受体CD44抗体单克隆放射性核素显像
Keywords:
tumor EGFR CD44 antibody monoclonal technetium radionuclideimaging
分类号:
R730.45; R734.2; R817.4
文献标志码:
A
摘要:
目的   评价99Tcm-EGFR-McAb及99Tcm-CD44-McAb单独及联合应用于荷人肺腺癌裸鼠的放射免疫显像效果。   方法   建立荷人肺腺癌裸鼠动物模型;采用直接法99Tcm标记抗EGFR和CD44的单克隆抗体,运用柱层析法对标记抗体分离、纯化,并对标记、纯化后的抗体特性进行鉴定;利用99Tcm-EGFR-McAb及99Tcm-CD44-McAb单独及联合进行荷人肺腺癌裸鼠显像及体内分布研究。   结果    99Tcm对EGFR-McAb和CD44-McAb的标记率分别为(91.5±3.8)%、(92.3±4.1)%,标记抗体比活度分别为(2.8±0.3) MBq/μl、(2.9±0.5) MBq/μl,放射化学纯度分别为(96.5±2.8)%、(96.2±3.1)%。放射免疫显像结果显示,肿瘤组织对2种标记抗体均有较高的摄取,2种标记抗体联合使用肿瘤部位的放射性浓聚明显高于2种标记抗体单独使用,通过ROI技术测得T/NT值分别为(5.58±0.46)、(2.72±0.22)、(2.30±0.18)。标记抗体的体内分布规律与显像结果一致。   结论    99Tcm-EGFR-McAb、99Tcm-CD44-McAb用于荷人肺腺癌裸鼠的放射免疫显像能得到较理想的靶/非靶比值,2种标记抗体的联合应用,可在一定程度上提高靶/非靶比值,优于一种标记抗体的单独使用。
Abstract:
Objective   To evaluate the effects of radioimmunoimaging with 99Tcm-EGFR-McAb or 99Tcm-CD44-McAb or combined application of both on nude mice bearing lung adenocarcinoma.    Methods   Animal model of nude mice bearing lung adenocarcinoma was established. The direct labeling method of 99Tcm was applied to labeling monoclonal antibodies for EGFR and CD44, and then the properties of the labeling antibodies purified by SephadexG50 Column were identified. The radio-immuno-image as well as body distribution concerning nude mice bearing lung adenocarcinoma was studied by application of 99Tcm-EGFR-McAb or 99Tcm-CD44-McAb alone or combined application of both.     Results   The labeling rates of 99Tcm for EGFR-McAb and CD44-McAb were (91.5±3.8)% and (92.3±4.1)% respectively. Specific activities of the labeled antibodies were (2.8±0.3)MBq/g and (2.9±0.5)MBq/g, respectively. The rates of radiochemical purity were (96.5±2.8)% and (96.2±3.1)%, respectively. The tumor tissue had high intake of the two labeled antibodies according to radioimmunoimaging result. The radioactivity concentration of the combined application of the two labeled antibodies was obviously higher than that of the single application. The T/NT relative values measured through ROI technique were 5.58±0.46, 2.72±0.22, and 2.30±0.18, respectively. The body distribution result of the labeled antibodies and their imaging results were basically identical.     Conclusion   The optimal target and non-target ratios could be obtained by application of 99Tcm-EGFR-McAb or 99Tcm- CD44-McAb alone in nude mice bearing lung adenocarcinoma. The target and non-target ratios could be enhanced through the combined application of the two antibodies.

参考文献/References:

段东, 李少林, 朱玉泉, 等.99Tc-EGFR-McAb及99Tc-CD44-McAb单独及联合用于荷人肺腺癌裸鼠的显像研究[J]. 第三军医大学学报,2009,31(13):1287-1290.

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更新日期/Last Update: 2009-06-16