[1]夏季,蔡晋,邓少丽,等.蛋白芯片联合检测5项结核抗体在结核病诊断中的价值[J].第三军医大学学报,2009,31(03):220-222.
 XIA Ji,CAI Jin,DENG Shao-li,et al.A protein chip detecting 5 antibodies against mycobacterium tuberculosis: evaluation in diagnosis of tuberculosis[J].J Third Mil Med Univ,2009,31(03):220-222.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第03期
页码:
220-222
栏目:
论著
出版日期:
2009-02-15

文章信息/Info

Title:
A protein chip detecting 5 antibodies against mycobacterium tuberculosis: evaluation in diagnosis of tuberculosis
作者:
夏季蔡晋邓少丽黄恒柳陈伟杨通怀
第三军医大学:大坪医院野战外科研究所检验科,西南医院检验科
Author(s):
XIA Ji CAI Jin DENG Shao-li HUANG Heng-liu CHEN Wei YANG Tong-huai
Department of Clinical Laboratory, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, Department of Clinical Laboratory, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
关键词:
结核分枝杆菌结核抗体蛋白芯片
Keywords:
mycobacterium tuberculosis antibodies protein chip
分类号:
R378.911; R392.11; R446.11
文献标志码:
A
摘要:
目的    探讨结核分枝杆菌抗LAM、38 kD、16 kD、CFP10、ESAT-6 5项抗体的检测对临床的应用价值。    方法    检测50例结核病、80例非结核病患者血清,应用蛋白芯片识别仪检测受试者血清中5项结核抗体,并对诊断试验结果进行分析评价。    结果    130例临床标本显示,以任一抗体检测阳性诊断为结核病阳性,则诊断结核病的灵敏度为92.0%,特异度为77.5%,正确率为83.1%,Youden指数为69.5%。    结论    蛋白芯片联合检测5项结核抗体具有较高的诊断价值,与“金标准”诊断的一致性较好,且取材简易、操作方便、历时较短,可以作为临床上诊断结核病特别是肺外结核病的一项重要辅诊项目。
Abstract:
Objective    To detect 5 antibodies against tuberculoprotein, LAM, CFP10, ESAT-6 and 2 proteins at molecular weight of 38  kD and 16 kD by a protein chip and explore its clinical value in diagnosis of tuberculosis.     Methods    The serum antibodies from 50 tuberculosis patients and 80 non-tuberculosis patients were analyzed by this protein chip, and the diagnostic value of the test was evaluated.     Results    As was shown in the 130 clinical samples, the sensitivity, specificity, accuracy and Youden index of the diagnostic test were 92.0%, 77.5%, 83.1% and 69.5% respectively.     Conclusion    The tuberculosis protein chip assay is of high value in diagnosis of both pulmonary and non-pulmonary tuberculosis patients, and has good coincidence with gold standard. It is time-saving and easy to sampling and practicing, and can be regarded as supplemental item for non-pulmonary tuberculosis.

参考文献/References:

夏季,蔡晋,邓少丽,等. 蛋白芯片联合检测5项结核抗体在结核病诊断中的价值[J]. 第三军医大学学报, 2009, 31(3):220-222.

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更新日期/Last Update: 2009-01-17