[1]闵敏,陈东风,王军,等.葡萄糖调节蛋白78在大鼠非酒精性脂肪性肝炎中的表达及意义[J].陆军军医大学学报(原第三军医大学学报),2009,31(01):79-81.
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葡萄糖调节蛋白78在大鼠非酒精性脂肪性肝炎中的表达及意义(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第01期
页码:
79-81
栏目:
论著
出版日期:
2009-01-15

文章信息/Info

Title:
Expression and significance of glucose regulated protein GRP78 in nonalcoholic steatohepatitis of rats
作者:
闵敏; 陈东风; 王军; 艾正琳; 樊丽琳; 严丽丽
第三军医大学大坪医院野战外科研究所消化内科
Author(s):
MIN Min;  CHEN Dong-feng;  WANG Jun;  AI Zheng-lin;  FAN Li-lin;  YAN Li-li
Department of Gastroenterology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China
关键词:
非酒精性脂肪性肝炎; 内质网应激; 葡萄糖调节蛋白
Keywords:
nonalcoholic steatohepatitis;  endoplasmic reticulum stress;  glucose regulated protein 78
分类号:
R341; R575.502
文献标志码:
A
摘要:
目的    观察高脂饮食诱导大鼠非酒精性脂肪性肝炎（nonalcoholic steatohepatitis,NASH）过程中内质网应激标志物葡萄糖调节蛋白78的表达变化，探讨内质网应激在NASH发病机制中的作用。    方法    建立大鼠高脂饮食诱导NASH模型，检测血清ALT、AST、FFA、TG和肝组织MDA的变化，用RT-PCR与Western blot方法检测NASH形成过程中肝组织GRP78在基因和蛋白水平的表达变化。    结果    NASH组大鼠血清ALT、AST、FFA、TG和肝组织MDA水平均较对照组有不同程度的升高。肝组织GRP78基因和蛋白表达也随NASH加重而增强，16周时升高最为显著（P<0.01）。    结论    高脂饮食可诱导GRP78表达增强，启动内质网应激，导致脂质异常沉积，其可能与NASH的发病机制密切相关。
Abstract:
Objective    To investigate the expression of endoplasmic reticulum molecular chaperone, glucose regulated protein 78 (GRP78), in the liver tissues during fatty diet-induced nonalcoholic steatohepatitis (NASH) in rats and explore the role of endoplasmic reticulum stress in pathogenesis of nonalcoholic steatohepatitis.     Methods    Rat model of nonalcoholic steatohepatitis were established with fatty dieting. Blood samples were collected to detect serum aspartate transaminase (AST), alanine aminotransferase (ALT), free fatty acid (FFA) and triglyceride (TG). Liver tissues were obtained to measure malondialdehyde (MDA) content. The expression of GRP78 in the liver was detected with RT-PCR and Western blot analysis.     Results    Compared with the control group, the serum contents of ALT, AST, FFA and TG and liver content of MDA in NASH were increased. The expression of GRP78 at protein and gene levels were increased in NASH in a time-dependent manner, and reached peak in 16 weeks (P<0.01).     Conclusion    Enhanced expression of GRP78 could be induced by fatty diet. The excessive expression of GRP78 could result in the endoplasmic reticulum stress, and thus lead to fatty acid abnormal deposition, which might be closely involved in NASH.

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更新日期/Last Update: 2009-01-06