[1]姚婕,张晓兵,王珏,等.白血病细胞雌激素受体多个启动子甲基化的实验研究[J].第三军医大学学报,2008,30(19):1799-1802.
 YAO Jie,ZHANG Xiao-bing,WANG Jue,et al.Methylation profile of estrogen receptor promoters gene in leukemia cells[J].J Third Mil Med Univ,2008,30(19):1799-1802.
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白血病细胞雌激素受体多个启动子甲基化的实验研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第19期
页码:
1799-1802
栏目:
论著
出版日期:
2008-10-10

文章信息/Info

Title:
Methylation profile of estrogen receptor promoters gene in leukemia cells
作者:
姚婕张晓兵王珏府伟灵
第三军医大学西南医院检验科
Author(s):
YAO Jie ZHANG Xiao-bing WANG Jue FU Wei-ling
Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
关键词:
白血病雌激素受体基因DNA甲基化启动子
Keywords:
leukemia estrogen receptor DNA methylation promoter
分类号:
R329.26; R730.23; R733.7
文献标志码:
A
摘要:
目的    检测白血病细胞雌激素受体(estrogen receptors, ER)基因启动子区CpG岛甲基化状态情况,探讨雌激素受体基因多个启动子区包括ERα ( ERα-A, -B and -C)和ERβ甲基化与其表达异常的联系。    方法    应用RT-PCR检测6种白血病细胞株以5′-杂氮-2-脱氧胞苷( 5′-Aza-Dc) 去甲基化前后ER基因表达活性并应用甲基化特异性PCR技术(methylation specific PCR, MSP)检测10例正常人白细胞和6种白血病细胞株以5′-Aza-Dc去甲基化前后ER基因甲基化状态。    结果    ER各启动子在白血病细胞株中均出现甲基化或半甲基化带,但只有ERα-A基因表达沉默而且在正常人白细胞中呈未甲基化状态,在以5′-Aza-Dc处理细胞株后ERα-A基因表达恢复。    结论    白血病细胞的雌激素受体基因启动子区存在高甲基化现象并导致其基因表达沉默,可能为白血病的致病机制分析提供了一个新的方向,提示ERα-A基因启动子CPG岛的甲基化有可能成为白血病的新的参考标记物。
Abstract:
Objective    To investigate the methylation status of promoter CpG islands of the estrogen receptors (ERs) in leukemia cell lines, including 3 promoters of ERα (ERα-A, -B and -C) and one promoter of ERβ, and explore the possible relationship between the mRNA expressions of the promoters and the CpG methylation.     Methods    With RT-PCR and methylation specific PCR (MSP), the mRNA expressions of above promoters and their status of methylation in leukemia cell lines HL-60, K562, Jurkat, THP-1, U937 and MOLT-410, and health controls with or without treatment of 5-AzA-2′-Deoxycitidine (5-Aza-DC).     Results    Promoters of ER were all methylated or semi-methylated in the 6 strains of leukemia cells. Only the ERα-A was inactivated in these leukemia cell lines and was unmethylated in all 10 health controls, and the expression was recovered after the treatment of 5′-Aza-DC.     Conclusion    The CpG islands of ER promoter are hypermethylated in 6 strains of leukemia cells, and this inactivate the expression of ERα-A, which can be activated by the treatment of 5′-Aza-DC.

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更新日期/Last Update: 2008-09-26