[1]万莹铧,司维柯,杜业军,等.辐射对L929细胞生长及CCN1基因表达的影响[J].第三军医大学学报,2009,31(03):205-208.
 WAN Ying-hua,SI Wei-ke,DU Ye-jun,et al.Effects of radiation on growth and CCN1 expression of mice fibroblast cell line L929[J].J Third Mil Med Univ,2009,31(03):205-208.
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辐射对L929细胞生长及CCN1基因表达的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第03期
页码:
205-208
栏目:
论著
出版日期:
2009-02-15

文章信息/Info

Title:
Effects of radiation on growth and CCN1 expression of mice fibroblast cell line L929
作者:
万莹铧司维柯杜业军李招权潘静赵宸李军粟永萍
第三军医大学:医学检验系临床血液学教研室,军事预防医学院防原医学教研室,全军复合伤研究所,创伤、烧伤与复合伤国家重点实验室
Author(s):
WAN Ying-hua SI Wei-ke DU Ye-jun LI Zhao-quan PAN Jing ZHAO Chen LI Jun SU Yong-ping
Department of Clinical Hematology, College of Laboratory Medicine, Department of Nuclear Prevention Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China
关键词:
辐射电离细胞系L929Cyr61/CCN1
Keywords:
radiation ionizing fibroblasts L929 cysteine-rich 61
分类号:
R329.26; R394.2; R818.02
文献标志码:
A
摘要:
目的    通过观察辐射对小鼠皮肤成纤维细胞系L929生长及CCN1基因表达的影响,探讨CCN1基因与辐射损伤的关系。    方法    体外培养L929细胞系,以4 Gy 60Coγ射线照射为辐射组,并设对照组,MTT法测细胞增殖变化,平板克隆形成实验检测克隆形成能力,流式细胞术测细胞周期,RT-PCR及免疫细胞化学法检测L929细胞中CCN1基因的表达情况。    结果    辐射组细胞辐照2 d增殖抑制明显(P<0.01),克隆形成也明显受到抑制(P<0.05);辐射后24 h,细胞出现G2期停滞;辐射后6 h L929细胞CCN1的mRNA表达水平明显降低(P<0.01),至24 h开始回调(P<0.05);辐照后24~48 h,细胞CCN1蛋白的表达水平明显下降(P<0.01)。    结论    因辐照生长受到抑制的小鼠成纤维细胞L929,其CCN1基因的表达水平下调,提示CCN1基因的表达水平低下是辐射导致细胞生长抑制的因素之一。
Abstract:
Objective    To observe the effects of radiation on the growth and expression of cysteine-rich 61 (Cyr61/CCN1) of L929 cells and investigate the relationship between CCN1 expression and radiation injury.     Methods    L929 cells were cultured and divided into 2 groups, cells irradiated with 4 Gy γ-irradiation as radio-group and untreated cells as control group. The cell proliferation was measured by MTT assay and plate colony formation testing. Flow cytometry was utilized to quantify the cell cycle distribution. CCN1 expression at protein and mRNA levels were determined by immunocytochemistry (ICC) and RT-PCR respectively.     Results    Significant inhibition of proliferation (P<0.01) and colony formation (P<0.05) were found in cells of radio-group. Meanwhile, cells were arrested at G2 phase at 24 h after radiation. The expression of CCN1 mRNA was down-regulated at 6 h (P<0.01), but moderately increased at 24 h (P<0.05). The expression of CCN1 protein was obviously decreased at 24 to 48 h after radiation (P<0.01).     Conclusion    Radiation inhibits the growth and CCN1 expression of cultured fibroblasts, indicating CCN1 may be involved in the suppression of fibroblasts.

参考文献/References:

万莹铧,司维柯,杜业军,等. 辐射对L929细胞生长及CCN1基因表达的影响[J]. 第三军医大学学报, 2009, 31(3):205-208.

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更新日期/Last Update: 2009-01-17