[1]伊海英,孙晓艳,付小兵,等.胎儿皮肤发育中成纤维细胞生长因子受体-2表达的免疫组织化学研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(21):2008-2010.
 YI Hai-ying,SUN Xiao-yan,FU Xiao-bing,et al.Distribution and expression of FGFR2 in human fetal skin by immunohistochemical method[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(21):2008-2010.
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胎儿皮肤发育中成纤维细胞生长因子受体-2表达的免疫组织化学研究(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第21期
页码:
2008-2010
栏目:
论著
出版日期:
2008-11-15

文章信息/Info

Title:
Distribution and expression of FGFR2 in human fetal skin by immunohistochemical method
作者:
伊海英孙晓艳付小兵任明姬张广田刘惠玲
内蒙古医学院基础医学院组织胚胎学教研室;解放军总医院基础医学研究所
Author(s):
YI Hai-ying SUN Xiao-yan FU Xiao-bing REN Ming-ji ZHANHG Guang-tian LIU Hui-ling
Department of Histology and Embryology, Inner Mongolian Medical College, Hohhot 010059; Institute of Basic Medical Science, General Hospital of PLA, Beijing 100853, China
关键词:
胚胎发育FGFR2免疫组织化学再生
Keywords:
embryonic development fibroblast growth factor receptor 2 immunohistochemical staining regeneration
分类号:
R321; R322.99; R329.2
文献标志码:
A
摘要:
目的    研究不同发育时期胎儿皮肤中成纤维细胞生长因子受体-2(fibroblast growth factor receptor 2,FGFR2)的表达及分布,探索FGFR2在皮肤发生、发育中的作用。    方法    对不同发育时期胎儿皮肤取材、固定,制成石蜡切片,S-P法检测FGFR2的表达。    结果    胚胎发育期FGFR2在表皮细胞表达较弱,甚至呈阴性表达。表皮分层期时,表皮层开始增厚,并可见毛囊的初级原基;FGFR2在表皮层和毛囊初级原基内呈弱阳性表达。毛囊形成期时,毛囊的结构初步形成,体积细小,形态幼稚;FGFR2主要在毛母质细胞内表达,并且随着胎龄增加,表达范围逐渐扩大,表达量逐渐增多。毛囊发育成熟期后,毛囊的结构发育相对成熟,FGFR2则在表皮层、毛细血管内皮细胞及皮脂腺的导管部均有表达,尤其在毛母质细胞呈强阳性表达。    结论    FGFR2在胎儿皮肤中的表达与分布与毛囊的发生、发育密切相关。FGFR2的表达上调可能促进毛囊干细胞的增殖,并诱导其定向分化形成终末组织功能细胞。
Abstract:
Objective    To investigate the expression and distribution characteristics of fibroblast growth factor receptor 2 (FGFR2) in the human fetal skin during various stages of the gestation, and to explore its biological significance in the cutaneous development and wound healing process.     Methods    Fetal skin samples at different pregnant terms were obtained with informed consent and the approval of the Ethics Committee of Inner Mongolian Medical College. The expression of FGFR2 in these skin samples was analyzed by using immunohistochemical method.     Results    Positive immunohistochemical signals of FGFR2 were found in fetal skins, which were closely related to the developmental patterns of hair follicles. The expression of FGFR2 was first detected in the cellular membrane of epidermal cells in the undifferentiated epidermis during the embryonic period. Coinciding with the period of the stratification of the epidermis, FGFR2 was observed in the newly formed epidermal layers and primary hair germs. During the period of follicular keratinization, the differentiated spinous and granular layers were very prominent, and the epidermis reached to its maximal thickness at this time, meanwhile, the follicular buds also progressed through a rapid morphogenesis. The appearance of FGFR2 was observed exactly in the epithelial-mesenchymal interaction of hair bulbs at high level. Furthermore, during the period of follicular keratinization, the expression of FGFR2 was detectable in the epidermal layers, capillary endothelium, ducts of sebaceous glands, as well as the epithelial-mesenchymal interactions of follicles. However, the differential expression was coincided with the maturation and differentiation process of hair follicles at the end of this period.     Conclusion    The differential expression of FGFR2 in human fetal skins is closely related to the developmental patterns of hair follicles, which contributes to the maintenance and proliferation of follicle precursors, and regulates them to differentiate into terminal functional cells in the hair follicles.

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更新日期/Last Update: 2008-10-20