[1]余剑,曹红卫,梁华平,等.创伤患者外周血单核细胞自噬变化的研究[J].第三军医大学学报,2008,30(21):1994-1997.
 YU Jian,CAO Hong-wei,LIANG Hua-ping,et al.Autophagy of peripheral monocytes following trauma: study on 51 cases[J].J Third Mil Med Univ,2008,30(21):1994-1997.
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创伤患者外周血单核细胞自噬变化的研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第21期
页码:
1994-1997
栏目:
论著
出版日期:
2008-11-15

文章信息/Info

Title:
Autophagy of peripheral monocytes following trauma: study on 51 cases
作者:
余剑曹红卫梁华平张连阳
第三军医大学:大坪医院野战外科研究所全军战创伤中心,西南医院中心实验室,大坪医院野战外科研究所第一研究室,创伤、烧伤与复合伤国家重点实验室
Author(s):
YU Jian CAO Hong-weiLIANG Hua-ping ZHANG Lian-yang
Trauma Center, State Key Laboratory of Trauma, Burns and Combined Injury, Department 1, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042; Central Laboratory, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
关键词:
自噬创伤单核细胞糖代谢
Keywords:
autophagy trauma monocytes glycometabolism
分类号:
R329.25; R331.14; R641.02
文献标志码:
A
摘要:
目的    研究创伤患者外周血单核细胞的自噬变化。    方法     ①根据损伤严重度评分(injury severity score,ISS)或简明损伤定级标准(abbreviated injury scale,AIS)将创伤患者分为轻度创伤组、中度创伤组、严重创伤组,同时设立健康对照组,分别于创伤后24 h、3、5、10 d采集外周血,采用密度梯度离心结合贴壁分离法分离创伤患者外周血单核细胞,应用激光共聚焦显微镜和透射电镜观察细胞自噬现象。②将分离得到的单核细胞进行MDC染色并裂解,应用荧光分光光度计(激发光波长380 nm、发射光波长525 nm)测定荧光强度进行定量分析。③于创伤后24 h、3、5 d检测患者血糖水平,分析创伤后自噬与血糖的相关性。    结果    与健康对照组相比,创伤患者外周血单核细胞自噬水平明显升高(P<0.01),创伤后外周血单核细胞自噬水平与创伤程度成正比,与创伤后血糖呈正相关(轻度创伤组:r=0.962,中度创伤组:r=0.928,重度创伤组:r=0.973,P<0.05)。    结论    创伤后通过调节单核细胞自噬水平,使机体免疫功能得到恢复,从而改善预后。
Abstract:
Objective    To investigate the change of monocyte autophagy in patients after trauma.     Methods     ①According to Injury Severity Score (ISS) or Abbreviated Injury Scale (AIS), we divided our 51 subjected trauma patients into 3 groups, the minor injury group (n=21), the moderate trauma group (n=19) and the   severe trauma   group (n=11). Another 14 healthy volunteers served as control. We   collected the peripheral blood in 24 h, 3, 5 and 10 d after trauma, then harvested the monocytes by density gradient centrifugation with adherence separation. Then we observed the autophagy phenomenon by using laser confocal microscopy and transmission electron microscopy; ② After the isolated monocytes were dyed with MDC and lysed, they were detected and quantified for fluorescent intensity by fluorospectrophotometer (excitation wavelength 380 nm and emission wavelength 525 nm); ③Blood sugar level of all patients were measured in 24 h, 3 and 5 d respectively after trauma, and the correlation between autophagy and blood sugar was analyzed.     Results    Compared with control group, more autophagy in monocytes was observed and quantified in trauma patients than in health volunteers (P<0.01).     Conclusion    Autophagy in monocytes is associated with the severity of trauma, and has a positive correlation with blood sugar.

参考文献/References:

余剑, 曹红卫, 梁华平, 等. 创伤患者外周血单核细胞自噬变化的研究[J]. 第三军医大学学报, 2008, 30(21):1994-1997.

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更新日期/Last Update: 2008-10-20