[1]冯丽霞,李力,俞丽丽,等.妊娠肝内胆汁淤积症胎兔心肌细胞损伤的研究[J].第三军医大学学报,2009,31(04):334-337.
 FENG Li-xia,LI Li,YU Li-li,et al.Cardiac myocyte damage in fetus of rabbits with intrahepatic cholestasis pregnant[J].J Third Mil Med Univ,2009,31(04):334-337.
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妊娠肝内胆汁淤积症胎兔心肌细胞损伤的研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第04期
页码:
334-337
栏目:
论著
出版日期:
2009-02-28

文章信息/Info

Title:
Cardiac myocyte damage in fetus of rabbits with intrahepatic cholestasis pregnant
作者:
冯丽霞李力俞丽丽郑英如郭建新易萍颜耀华
第三军医大学大坪医院野战外科研究所妇产科
Author(s):
FENG Li-xia LI Li YU Li-li ZHENG Ying-ru GUO Jian-xin YI Ping YAN Yao-hua
Department of Obstetrics and Gynecology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China
关键词:
肝内胆汁淤积妊娠胎儿心肌细胞
Keywords:
intrahepatic cholestasis pregnancy fetus cardiac myocyte
分类号:
R-332;R542.2;R714.255
文献标志码:
A
摘要:
目的    观察妊娠肝内胆汁淤积症(intrahepatic cholestasis of pregnancy,ICP)胎兔心肌细胞超微结构及细胞凋亡率,检测心肌腺苷三磷酸(ATP)、超氧化物歧化酶(SOD)、丙二醛(MDA)含量。    方法    16只孕兔,分为ICP组和对照组。ICP组自妊娠第22天,颈后皮下注射苯甲酸雌二醇0.1 mg·kg-1·d-1,对照组颈后皮下注射0.9%生理盐水,直至分娩。观察ICP组和对照组胎兔心肌细胞超微结构,检测心肌细胞ATP、SOD、MDA含量及细胞凋亡指数。    结果    电镜观察见ICP胎兔心肌细胞线粒体轻度肿胀、髓鞘样结构形成,细胞质内糖原颗粒分布减少,对照组超微结构无明显改变。ICP组胎心组织ATP、SOD含量减少,MDA含量和细胞凋亡数明显增多,与对照组相比差异均有统计学意义(P<0.05)。    结论    ICP胎兔存在心肌细胞损伤。
Abstract:
Objective    To investigate ultrastructure and apoptotic rate of fetal cardiac myocytes of rabbits with intrahepatic cholestasis of pregnancy (ICP)and the levels of adenosine triphosphate (ATP), superoxide dismutase (SOD) and malondialdehyde (MDA) in the myocardial tissues.     Methods    Sixteen pregnant rabbits were randomly divided into control group and ICP group. Rabbits in ICP group were subcutaneously injected with benzestrofol at a dose of 0.1 mg·kg-1·d-1 and control group with 0.9% saline solution from the 22nd gestation day to delivery day. The levels of ATP, SOD and MDA in myocardial tissues of ICP rabbit fetuses and control fetuses were determined. Ultrastructure and number of apoptosis cells were observed.     Results    Compared with controls, the levels of ATP and SOD in ICP rabbit fetuses’ cardiac myocytes significantly decreased (P<0.05), while the levels of MDA and the number of apoptosis cells in ICP rabbit fetuses’ cardiac myocytes significantly increased. Slight swelling, vacuolation and myelinoclasis in mitochondria, and decreased glycogen granules in cytoplasm were observed in ICP rabbit fetuses’ cardiac myocytes under electron microscope. However, these ultrastructural alteration was not observed in controls.     Conclusion    The damage of cardiac myocytes does exist in fetuses of rabbits with ICP.

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更新日期/Last Update: 2009-02-11