[1]张莉莉,李敬诚,王景周,等.PPAR-γ在高血压血管平滑肌细胞表型转化中的作用[J].第三军医大学学报,2009,31(04):311-314.
 ZHANG Li-li,LI Jing-cheng,WANG Jing-zhou,et al.Role of peroxisome proliferators-activated receptor-gamma in phenotypic transforming of vascular smooth muscle cells in hypertension[J].J Third Mil Med Univ,2009,31(04):311-314.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第04期
页码:
311-314
栏目:
论著
出版日期:
2009-02-28

文章信息/Info

Title:
Role of peroxisome proliferators-activated receptor-gamma in phenotypic transforming of vascular smooth muscle cells in hypertension
作者:
张莉莉李敬诚王景周谢鹏
重庆医科大学附属第一医院神经内科;第三军医大学大坪医院野战外科研究所神经内科
Author(s):
ZHANG Li-li LI Jing-cheng WANG Jing-zhou XIE Peng
Department of Neurology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016; Department of Neurology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China
关键词:
过氧化物酶体增殖物激活受体-γ血管平滑肌细胞血管重构表型转化
Keywords:
peroxisome proliferators activated receptor gamma vascular smooth muscle cells vascular remodeling phenotypic transforming
分类号:
R322.12;R394.3;R544.102
文献标志码:
A
摘要:
目的    观察过氧化物酶体增殖物激活受体-γ(peroxisome proliferator activated receptors-γ,PPAR-γ)在高血压血管平滑肌细胞(VSMC)表型转化中的作用。    方法    应用基因重组技术使PPAR-γ过表达和表达抑制,Western blot方法检测分化型VSMC标志物α-SMA和未分化型VSMC标志物OPN的蛋白表达,应用DNA合成率检测和细胞计数的方法测定细胞增殖率,常规病理切片和HE 染色观察血管形态学改变。    结果    ①PPAR-γ激动剂罗格列酮可降低SHR大鼠血压,并改善主动脉血管重构。②SHR大鼠主动脉中OPN表达增多而α-SMA表达减少,应用罗格列酮可抑制上述改变。③SHR大鼠来源的VSMC中OPN表达增多而α-SMA表达减少,细胞增殖率升高,PPAR-γ过表达使细胞增殖率降低,并抑制OPN和α-SMA的表达改变。    结论    PPAR-γ可抑制高血压VSMC向未分化表型的转化,可能是其改善高血压血管重构的重要机制。
Abstract:
Objective    To observe the effect of peroxisome proliferators activated receptor gamma (PPAR-γ) on phenotypic transforming of vascular smooth muscle cells (VSMC) in hypertension.     Methods    Spontaneous hypertension rats (SHR) and WKY rats both aged 4 months were included. SHR rats as well as WKY rats were divided to be fed with normal chow, and chow added with rosiglitazone (10 mg·kg-1·d-1) for 16 weeks. VSMC were isolated from SHR rats and WKY rats and cultured by patch-attaching method, then respectively divided into 3 groups after treated with genetic recombination technology: normal VSMC, PPARγ overexpressed VSMC and PPARγ silenced VSMC. Expressions of OPN and α-SMA, which respectively represent the undifferentiated and differentiated VSMC, were detected by Western blotting. Cell proliferation was determined by detecting DNA synthesis and cell counting. The changes of arteries were evaluated pathologically.     Results    Rosiglitazone decreased blood pressure and ameliorated vascular remodeling of aorta in SHR rats. Aorta of SHR showed an upregulation of OPN and downregulation of α-SMA, which could be inhibited by rosiglitazone. VSMC from SHR rats showed an upregulation of OPN and downregulation of α-SMA, and increased cell proliferation. These changes were all inhibited by rosiglitazone. In the cells that overexpressed PPARγ, the cell proliferation rate was lower, and the expressions of OPN and α-SMA were depressed, compared with the corresponding control cells.      Conclusion    PPAR-γ could inhibit the phenotypic transforming of VSMC, and this might be responsible for the amelioration of vascular remodeling in hypertension.

参考文献/References:

张莉莉, 李敬诚, 王景周, 等. PPAR-γ在高血压血管平滑肌细胞表型转化中的作用[J]. 第三军医大学学报, 2009, 31(4):311-314.

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更新日期/Last Update: 2009-02-11