[1]潘波,杨成明,曾春雨,等.吡咯烷二硫代氨基甲酸盐对大鼠缺血性心肌损伤后心室扩张及心衰的干预效应[J].陆军军医大学学报(原第三军医大学学报),2008,30(22):2128-2131.
 PAN Bo,YANG Cheng-ming,ZENG Chun-yu,et al.Protective effects of PDTC on ventricular dilatation and heart failure induced by experimental myocardial ischemic injury in rats[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(22):2128-2131.
点击复制

吡咯烷二硫代氨基甲酸盐对大鼠缺血性心肌损伤后心室扩张及心衰的干预效应(/HTML )
分享到:

陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第22期
页码:
2128-2131
栏目:
论著
出版日期:
2008-11-30

文章信息/Info

Title:
Protective effects of PDTC on ventricular dilatation and heart failure induced by experimental myocardial ischemic injury in rats
作者:
潘波杨成明曾春雨王旭开熊秀勤何多芬
第三军医大学大坪医院野战外科研究所:心血管内科,超声诊断科
Author(s):
PAN Bo YANG Cheng-ming ZENG Chun-yu WANG Xu-kai XIONG Xiu-qin HE Duo-fen
Department of Cardiology, Department of Ultrasound Diagnosis, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China
关键词:
核因子-κB抑制剂单核细胞趋化蛋白-1心肌缺血性损伤心室扩张心衰
Keywords:
nuclear factor-κB inhibitor monocyte chemoattractant protein-1 myocardial ischemic injury ventricular dilatation heart failure
分类号:
R541.62;R542.2;R972
文献标志码:
A
摘要:
目的    研究核因子-κB(nuclear factor-κB, NF-κB)抑制剂——吡咯烷二硫代氨基甲酸盐(pyrrolidine dithiocarbamate, PDTC)对异丙肾上腺素(isoprenaline, ISO)所致大鼠缺血性心肌损伤后心室扩张、心衰的防治作用及其作用机制。    方法    应用大剂量ISO建立大鼠缺血性心肌损伤模型。70只雄性Wistar大鼠利用随机数字表法分为3组:对照组20只,实验组(ISO组)30只,治疗组(ISO +PDTC组)20只。检查心肌病理形态学、心肌NF-κB激活情况、心肌单核细胞趋化蛋白-1(MCP-1)mRNA表达情况及血清MCP-1水平,并在实验第14天进行超声心动图检查,观察心室扩张及心功能变化情况。    结果    ISO所致大鼠缺血性心肌损伤后心肌NF-κB被激活,心肌MCP-1 mRNA表达增加(P<0.01),血清MCP-1增高(P<0.01);PDTC能够抑制NF-κB的激活,下调心肌MCP-1 mRNA的表达及血清MCP-1水平(P<0.01),减轻心肌炎症反应,减小心肌细胞损伤范围,但同时可导致肉芽组织替代受损心肌延迟;实验第14天,治疗组左心室收缩末期内径(LVESD)较实验组减小(P<0.05),射血分数(EF)优于实验组(P<0.05)。    结论    应用PDTC能够对心肌缺血性损伤后的心室扩张、心功能损害起到一定的防治作用,该作用可能主要得益于在心肌损伤急性期对心肌细胞的保护作用,其机制可能与抑制NF-κB的激活进而下调MCP-1的表达、减轻心肌炎症损伤有关。
Abstract:
Objective    To study the protective effects of nuclear factor-κB inhibitor, pyrrolidine dithiocarbamate (PDTC), on ventricular dilatation and heart failure induced by experimental myocardial ischemic injury in rats and its mechanisms.     Methods    High dose of isoprenaline (ISO) was used to induce experimental myocardial ischemic injury in rats. Seventy male Wistar rats were divided into three groups: control group (20 rats), ISO group (30 rats) and ISO+PDTC group (20 rats). Myocardial pathomorphologic changes, NF-κB p65 activation, MCP-1 mRNA expression and serum MCP-1 were investigated. Then ventricular dilatation and left ventricular (LV) function were assessed by echocardiography on day 14 of the experiment.     Results    Myocardial NF-κB was activated, and MCP-1 mRNA expression and serum MCP-1 increased markedly after myocardial ischemic injury induced by ISO (P<0.01). PDTC inhibited the activation of myocardial NF-κB and significantly decreased myocardial MCP-1 mRNA expression (P<0.01), serum MCP-1 (P<0.01), myocardial inflammation and injury. On the other hand, PDTC delayed replacement of the damaged myocardium with granulation tissue. On day 14 of the experiment, a marked decrease in left ventricular end-systolic dimension (LVESD) and a significant increase in percentage of ejection fraction were observed in ISO+PDTC group compared with ISO group (P<0.05).     Conclusion    The protective effects of PDTC on ventricular dilatation and heart failure may mainly be acquired from the acute stage of heart injury, which mechanisms are probably associated with inhibited activation of myocardial NF-κB, decreased myocardial MCP-1 mRNA expression, serum MCP-1, lessened myocardial inflammation and injury.

相似文献/References:

[1]李琴山,刘洋,冯赞杰,等.单核细胞趋化蛋白-1对人脐静脉内皮细胞凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(17):1682.
 LI Qin-shan,LIU Yang,FENG Zan-jie,et al.Monocyte chemotactic protein-1 induces the apoptosis of human umbilical vein endothelial cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):1682.
[2]卢志顺,余晓,唐俊利,等.胆固醇对血管内皮细胞的损伤[J].陆军军医大学学报(原第三军医大学学报),2006,28(16):1679.
[3]聂永梅,陈槐卿,成敏,等.兔动脉粥样硬化斑块处单核细胞趋化蛋白-1的表达[J].陆军军医大学学报(原第三军医大学学报),2006,28(15):1566.
[4]魏倩萍,邓华聪,赵劼,等.亚硒酸钠对大鼠肾小球系膜细胞HBZY-1单核细胞趋化蛋白-1 mRNA表达的影响[J].陆军军医大学学报(原第三军医大学学报),2005,27(14):1446.
[5]魏平,梁瑞凯,谭明红,等.2型糖尿病肾病大鼠肾脏MCP-1与氧化应激相关性研究[J].陆军军医大学学报(原第三军医大学学报),2011,33(19):2021.
 Wei Ping,Liang Ruikai,Tan Minghong,et al.Correlation on renal MCP-1 and oxidative stress in rats with type 2 diabetic nephropathy[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(22):2021.
[6]彭家和,刘红,张迁,等.小鼠MCP-1基因启动子的克隆及核受体FXR下调其活性初步分析[J].陆军军医大学学报(原第三军医大学学报),2010,32(01):33.
 Peng Jiahe,Liu Hong,Zhang Qian,et al.Cloning of mouse monocyte chemoattractant protein-1 promoter and down-regulation of its activity by farnesoid X receptor[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(22):33.
[7]程莹,母义明,汪保安,等.二甲双胍减轻软脂酸诱导的血管内皮细胞损伤机制探讨[J].陆军军医大学学报(原第三军医大学学报),2008,30(14):1357.
 CHENG Ying,MU Yi-ming,WANG Bao-an,et al.Protective mechanism of metformin in process of human umbilical vein endothelial cell injury induced by palmitic acid in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(22):1357.
[8]郜攀,黄岚,郭瑞威,等.B型利钠肽对小鼠腹腔巨噬细胞MCP-1蛋白表达的双向调节作用[J].陆军军医大学学报(原第三军医大学学报),2008,30(15):1452.
 GAO Pan,HUANG Lan,GUO Rui-wei,et al.Effects of B-type natriuretic peptides on expression of monocyte chemoattractant protein-1 in rat peritoneal macrophages in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(22):1452.
[9]马洪,李旭良,魏光辉,等.小儿肾积水尿EGF、MCP-1水平的检测及意义[J].陆军军医大学学报(原第三军医大学学报),2009,31(12):1227.
[10]胡旭,初同伟,廖通权,等.单核细胞趋化蛋白-1在恶性骨肿瘤中高表达[J].陆军军医大学学报(原第三军医大学学报),2011,33(09):924.
 Hu Xu,Chu Tongwei,Liao Tongquan,et al.High expression of monocyte chemotactic protein-1 in malignant bone tumor[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(22):924.

更新日期/Last Update: 2008-10-30