[1]张涛,汤为学,蔡辉,等.不同转移潜能乳腺癌细胞亚系的建立与生物学特性的分析[J].第三军医大学学报,2008,30(18):1726-1729.
 ZHANG Tao,TANG Wei-xue,CAI Hui,et al.Establishment of breast cancer cell sublines with different potential of metastasis and their biological characteristics[J].J Third Mil Med Univ,2008,30(18):1726-1729.
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不同转移潜能乳腺癌细胞亚系的建立与生物学特性的分析(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第18期
页码:
1726-1729
栏目:
论著
出版日期:
2008-09-24

文章信息/Info

Title:
Establishment of breast cancer cell sublines with different potential of metastasis and their biological characteristics
作者:
张涛汤为学蔡辉李少林
重庆医科大学:附属第一医院肿瘤科,基础医学院病理生理学教研室,基础医学院核医学教研室
Author(s):
ZHANG Tao TANG Wei-xue CAI Hui LI Shao-lin
Department of Oncology, The First Affiliated Hospital;Department of Pathophysiology, Department of Nuclear Medicine, Faculty of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China
关键词:
乳腺癌肿瘤转移细胞株生物学特性
Keywords:
breast cancer neoplasm metastasis cell subclone biological characteristics
分类号:
R73-354;R730.23;R737.9
文献标志码:
A
摘要:
目的    探讨利用细胞穿透人工基质膜能力的差异筛选不同转移潜能乳腺癌细胞的可行性,并分析二者生物学特性差异。    方法    人乳腺癌细胞系MDA-MB-435s经过连续人工基质膜侵袭试验后获得高、低转移潜能细胞株,并应用细胞生长曲线测定、侵袭力、黏附率实验,促血管形成实验分析所筛选出的不同转移潜能乳腺癌细胞的生物学性质的差异。    结果    获得2株转移潜能不同的乳腺癌细胞株,它们具有差异明显的体外生长速度、侵袭力、黏附力和促血管形成能力。    结论    利用细胞穿透人工基底膜能力的差异能够筛选出高、低转移潜能的乳腺癌细胞。
Abstract:
Objective    To investigate the validity of selecting subclones of human breast cancer cells with high- and low-metastatic potentiality, based on their different ability of penetrating the matrigel in vitro, and analyze their biological characteristics.     Methods    Continuous in vitro invasion assays in matrigel were applied to screen two subclones of human breast cancer cell line MDA-MB-435s, characterized by high- and low-metastatic potentiality. Cell growth curve assay, invasion assay, adhesion assay and angiopoiesis-promoting assay were conducted to explore the different biological properties of these two subclone cells.     Results    Two subclones of MDA-MB-435s, of high- and low-metastatic potentiality respectively, were obtained through continuous in vitro invasion assay, manifesting significant difference in growth rate, invasion ability, adhesion ability and angiopoiesis-promoting ability.     Conclusion    It is feasible to obtain subclones of human breast cancer cells of high- and low-metastatic potentiality by utilizing their difference in penetrating the matrigel in vitro.

参考文献/References:

张涛,汤为学,蔡辉,等.  不同转移潜能乳腺癌细胞亚系的建立与生物学特性的分析[J]. 第三军医大学学报, 2008, 30(18):1726-1729.

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更新日期/Last Update: 2008-09-11