[1]刘川,吴小候,张唯力,等.Livin反义寡核苷酸诱导人肾癌细胞凋亡及其机制的研究[J].第三军医大学学报,2008,30(17):1622-1625.
 LIU Chuan,WU Xiao-hou,ZHANG Wei-li,et al.Antisense oligonucleotide targeting livin induces apoptosis of human renal carcinoma cell 786-O in vitro and its mechanism[J].J Third Mil Med Univ,2008,30(17):1622-1625.
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Livin反义寡核苷酸诱导人肾癌细胞凋亡及其机制的研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第17期
页码:
1622-1625
栏目:
论著
出版日期:
2008-09-15

文章信息/Info

Title:
Antisense oligonucleotide targeting livin induces apoptosis of human renal carcinoma cell 786-O in vitro and its mechanism
作者:
刘川吴小候张唯力唐伟尹志康何云锋杜虎
重庆医科大学  :附属第二医院泌尿外科,  附属第一医院泌尿外科
Author(s):
LIU Chuan WU Xiao-hou ZHANG Wei-li TANG Wei YIN Zhi-kang HE Yun-feng DU Hu
Department of Urinary Surgery, The Second College of Clinical Medicine, Chongqing 400010, Department of Urology, College of Clinical Medicine, Chongqing Medical University, Chongqing 400016, China
关键词:
 Livin寡核苷酸类反义细胞凋亡肾肿瘤
Keywords:
livin oligonucleotides antisense apoptosis renal tumor
分类号:
R394.2; R73-362; R737.11
文献标志码:
A
摘要:
目的    探讨Livin反义寡核苷酸(ASODN)对人肾癌细胞株786-O Livin mRNA及蛋白表达的抑制作用以及对细胞凋亡的影响。    方法    设计合成全硫代磷酸化修饰的Livin ASODN,脂质体辅助转染至786-O细胞内。采用RT-PCR检测转染后Livin mRNA的变化,细胞免疫化学、激光扫描共聚焦显微镜观察Livin蛋白在细胞内的分布、转染后表达的变化及细胞形态的变化,流式细胞仪检测细胞凋亡率的变化,激酶法测定Caspase3活性的变化。    结果    转染Livin ASODN后,RT-PCR显示786-O细胞表达Livin mRNA减少(P<0.01),激光扫描共聚焦显微镜下见细胞表达Livin蛋白减少、细胞形态发生变化,流式细胞仪检测细胞凋亡率显著增加(P<0.01),Caspase3活性明显增加(P<0.01)。    结论    Livin ASODN能明显下调786-O细胞Livin基因的表达,诱导细胞凋亡。抑制Caspase3活性是Livin抑制细胞凋亡的途径之一。
Abstract:
Objective    To investigate the effect of antisense oligonucleotides (ASODN) targeting livin on the inhibition of livin mRNA and protein expression and the apoptosis of human renal carcinoma cell line 786-O cells.     Methods    Specific phosphorothioate antisense oligodeoxynucleotides targeting livin were synthesized and then transfected into 786-O cells. The expressions of livin mRNA were detected by RT-PCR. Expression and location of livin protein were observed by confocal laser scanning microscope (CLSM). Apoptosis rate of 786-O cells was investigated by flow cytometer. The activity of Caspase-3 was detected by colorimetric assay.     Results    After the transfection of ASODN, the expression of livin mRNA was decreased (P<0.01), the fluorescence signal of livin protein observed by CLSM was significantly decreased, the apoptosis rates detected by flow cytometry were significantly increased (P<0.01), and the caspase-3 activity was enhanced (P<0.01).     Conclusion    Livin ASODN could remarkably down-regulate the expression of livin gene in 786-O cells line and induce tumor cell apoptosis. These events are linked to the activation of caspase-3.

参考文献/References:

刘川,吴小候,张唯力,等 .     Livin反义寡核苷酸诱导人肾癌细胞凋亡及其机制的研究[J]. 第三军医大学学报, 2008, 30(17):1622-1625.

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更新日期/Last Update: 2008-09-16