[1]胡雁,周善璧.1, 25-二羟维生素D3抑制大鼠角膜移植术后免疫排斥反应[J].第三军医大学学报,2008,30(23):2211-2214.
 HU Yan,ZHOU Shan-bi.Prevention of corneal allograft rejection by 1, 25-dihydroxyvitamin D3 in rats[J].J Third Mil Med Univ,2008,30(23):2211-2214.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第23期
页码:
2211-2214
栏目:
论著
出版日期:
2008-12-15

文章信息/Info

Title:
Prevention of corneal allograft rejection by 1, 25-dihydroxyvitamin D3 in rats
作者:
胡雁 周善璧
重庆医科大学附属第一医院眼科
Author(s):
HU Yan ZHOU Shan-bi
Department of Ophthalmology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
关键词:
125-二羟维生素D3角膜移植白介素
Keywords:
1 25-dihydroxyvitamin D3 corneal transplantation interleukin
分类号:
R392.4; R772.2; R977.24
文献标志码:
A
摘要:
目的    研究1,25-二羟维生素D3对角膜移植术后免疫排斥反应的影响。    方法    以45只SD大鼠为受体,15只Wistar大鼠为供体建立穿透性角膜移植模型。受体SD大鼠随机分成实验组、实验对照组和对照组,每组15只。实验组及实验对照组行同种异体角膜移植,对照组行自体角膜移植。术后0~13 d实验组腹腔注射1,25-二羟维生素D31.0 μg·kg-1·d-1,对照组和实验对照组注射灭菌花生油2 ml/d;术后观察植片存活情况,并于术后14、21、30 d每组随机处死5只大鼠行植片病理学检查和ELISA检测外周血IL-1β、IL-2、IL-8、IL-10。    结果    对照组角膜植片存活时间为 (21.7±6.8) d,实验对照组为 (11.2±2.5) d,实验组为 (19.3±5.2) d,3组比较统计学差异非常显著 (P<0.01);病理学检查示实验组淋巴细胞浸润和新生血管均较实验对照组少;实验组外周血中IL-1β、IL-2、IL-8含量与实验对照组相比下降, IL-10含量增高,统计学差异非常显著 (P<0.01)。    结论    1,25-二羟维生素D3能在角膜移植后有效的保护植片,抑制排斥反应,延长植片存活时间。
Abstract:
Objective    To investigate the effects of 1, 25-dihydroxyvitamin D3 in the prevention of corneal allograft rejection.     Methods    Forty-five SD rats (only right eyes) were used as recipients, and 15 Wistar rats (double eyes) were used as donors. SD rats were randomly divided into 3 groups: the autograft control group, the allograft control group (receiving peanut oil as placebo), and  the allograft group were treated with 1, 25-dihydroxyvitamin D31.0 μg ·kg-1·d-1, 3 times/d for 13 d, i.p.). Drugs were injected intraperitoneally for 14 d after transplantation. Routine antibiotic eye drops were used. Grafts were observed by operating microscopy everyday after transplantation. Five SD rats in each group were killed respectively at the 14, 21, and 30 d postoperatively. Neovascularization and inflammation were evaluated with HE staining. ELISA was used to detect the contents of IL-1β, IL-2, IL-8, and IL-10 in the peripheral blood.     Results    The mean survival time (MST) of the graft was (21.7±6.8) d  in the autograft control group, (11.2±2.5) d in the allograft control group, and (19.3±5.2) d in the allograft group. There was statistically significance between the latter 2 groups (P<0.01). The allograft group had less neovascularization and inflammation than the allograft control group; the blood levels of IL-1β, IL-2, and IL-8 were markedly lower in the former than in the latter group, but IL-10 were significantly higher (P<0.01).     Conclusion    1, 25-dihydroxyvitamin D3 can prolong the survival time of corneal grafts by inhibiting the immune rejection of corneal transplantation.

参考文献/References:

胡雁, 周善璧. 1, 25-二羟维生素D3抑制大鼠角膜移植术后免疫排斥反应[J].  第三军医大学学报, 2008, 30(23):2211-2214.

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更新日期/Last Update: 2008-11-12