[1]储卫华,詹梦熊,朱刚,等.EDS对新生SD大鼠海马神经干细胞增殖分化的作用[J].第三军医大学学报,2008,30(19):1787-1790.
 CHU Wei-hua,ZHAN Meng-xiong,ZHU Gang,et al.Effects of ecdysterone on proliferation and differentiation of rat neural stem cells in vitro[J].J Third Mil Med Univ,2008,30(19):1787-1790.
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EDS对新生SD大鼠海马神经干细胞增殖分化的作用(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第19期
页码:
1787-1790
栏目:
论著
出版日期:
2008-10-10

文章信息/Info

Title:
Effects of ecdysterone on proliferation and differentiation of rat neural stem cells in vitro
作者:
储卫华詹梦熊朱刚陈志刘智唐卫华胡荣麻彤辉冯华
第三军医大学西南医院神经外科,全军神经系统疾病微创诊治中心;东北师范大学生命科学学院
Author(s):
CHU Wei-hua ZHAN Meng-xiong ZHU Gang CHEN Zhi LIU Zhi TANG Wei-hua HU Rong MA Tong-hui FENG Hua
Department of Neurosurgery, Southwest Hospital, Third Military Medical University,  Chongqing 400038; Institute of Life Science, Northeast Normal University, Changchun 130024, China
关键词:
蜕皮甾酮神经干细胞增殖分化
Keywords:
ecdysterone neural stem cells proliferation differentiation
分类号:
R285.5;R329.21;R329.25
文献标志码:
A
摘要:
目的    观察在不同剂量蜕皮甾酮(ecdysterone,EDS)作用下,体外培养的神经干细胞(neural stem cells,NSCs)增殖、分化情况。    方法    原代培养新生SD大鼠海马NSCs,经EDS处理后,传代细胞克隆形成率、四甲基偶氮唑盐比色法观察神经干细胞增殖能力的变化,免疫荧光细胞染色技术、流式细胞术检测神经干细胞分化情况。    结果    与对照组相比,中低剂量组(50、100、200 mg/L),对大鼠海马NSCs增殖能力无显著影响,而高剂量的EDS(400 mg/L及800 mg/L)干预组则明显抑制了NSCs的增殖。在分化条件下,EDS200 mg/L干预组显著提高NSCs向神经元分化的比例,其余各组无统计学差异。    结论    EDS能够调节体外培养的大鼠海马NSCs的增殖和分化水平,在合适的剂量下明显提高了神经元的分化比例。
Abstract:
Objective    To explore the effects of ecdysterone (EDS) on proliferation and differentiation of rat hippocampal neural stem cells (NSCs) in vitro.     Methods    NSCs of SD rat hippocampus were isolated and cultured in serum-free medium containing bFGF and EGF, then different doses of EDS were used to treat the NSCs. Clone counting and MTT assays were performed for the proliferation of NSCs; Immunofluorescence and flow cytometry were carried out for the cell differentiation.     Results    High doses of EDS (400 and 800 mg/L) treatment markedly inhibited the proliferation of rat hippocampal NSCs, while the low doses of EDS (50, 100 and 200 ng/L) exhibited no effects on the NSCs proliferation. Treatment of NSCs with 200 mg/L EDS markedly increased the neurogenesis of NSCs in differential medium, while NSCs of other doses did not.     Conclusion    EDS regulates the proliferation and differentiation of NSCs in vitro, whereas only 200 mg/L EDS can increase the neurogenesis of rat hippocampal NSCs.

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更新日期/Last Update: 2008-09-25