[1]吕智美,邓华聪,诸葛福媛,等.AP-1活化在高糖诱导肾小管上皮细胞转分化中的作用[J].第三军医大学学报,2008,30(22):2107-2110.
 LU Zhi-mei,DENG Hua-cong,ZHUGE Fu-yuan,et al.Effect of AP-1 on high glucose induced epithelial-mesenchymal-transition of human kidney proximal tubular epithelial cell[J].J Third Mil Med Univ,2008,30(22):2107-2110.
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AP-1活化在高糖诱导肾小管上皮细胞转分化中的作用(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第22期
页码:
2107-2110
栏目:
论著
出版日期:
2008-11-30

文章信息/Info

Title:
Effect of AP-1 on high glucose induced epithelial-mesenchymal-transition of human kidney proximal tubular epithelial cell
作者:
吕智美邓华聪诸葛福媛刘金波陈丹燕南静
重庆医科大学附属第一医院内分泌科
Author(s):
LU Zhi-mei DENG Hua-cong ZHUGE Fu-yuan LIU Jin-bo CHEN Dan-yan NAN Jing
Department of Endocrinology,The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
关键词:
活化蛋白-1肾小管上皮细胞转分化
Keywords:
activator protein-1 human kidney proximal tubular epithelial cell (HK2) epithelial-mesenchymal-transition
分类号:
R322.61;R329.25;R587.102
文献标志码:
A
摘要:
目的    探讨转录活化蛋白-1(activator protein-1, AP-1)在高糖诱导的肾小管上皮细胞表型转化中的作用。    方法    将体外培养的人肾小管上皮细胞(human kidney proximal tubular epithelial cell, HK2)分为正常对照组、高糖组、AP-1阻断组。光镜、透射电镜观察细胞形态的改变,免疫细胞化学法检测α-平滑肌肌动蛋白(α-SMA)和细胞角蛋白(cytokeratin,CK)的表达,用凝胶电泳迁移率改变分析法(electrophoretic mobility shift assay,EMSA)检测HK2细胞AP-1的改变,RT-PCR法检测CK mRNA的表达,Western blot检测α-SMA蛋白的表达。    结果    高糖作用48 h后,HK2细胞由椭圆形贴壁生长变为长梭形,胞体拉长,透射电镜下,细胞表面微绒毛明显减少,胞质内粗面内质网丰富,而AP-1阻断组较高糖组明显减轻;EMSA结果分析表明,在高糖刺激下,AP-1结合活力较正常对照组增加79.22%(P<0.05),而AP-1阻断剂可明显抑制AP-1的活化,较高糖组降低51.19%(P<0.05);免疫细胞化学和RT-PCR检测显示,高糖组和AP-1阻断组CK mRNA和蛋白水平明显降低(P<0.05),而AP-1阻断组显著高于高糖组(P<0.05);免疫细胞化学和Western blot检测显示,高糖组和AP-1阻断组α-SMA蛋白表达明显高于正常组(P<0.05),而AP-1阻断组显著低于高糖组(P<0.05)。    结论    高糖能够导致肾小管上皮细胞AP-1活化,诱导其表型转化。
Abstract:
Objective    To examine the effect of AP-1 on high glucose induced epithelial-mesenchymal-transition in cultured human kidney proximal tubular epithelial cell (HK2).      Methods    The cultured HK2 cells were divided into three groups: normal glucose group (NG), high glucose group (HG), activator protein-1 (AP-1) inhibited group (AG). The morphological changes of HK2 cells were observed by inverted microscope and electron microscope. The proteins of α-smooth muscle actin (α-SMA) and cytokeratin (CK) were determined by immunocytochemistry. The activity of activator protein-1 (AP-1) was assessed with electrophoretic mobility shift assay (EMSA). RT-PCR was used to detect the mRNA expression of CK. Western blot was used to detect the protein expression of α-SMA.     Results    The HK2 cells stimulated with high glucose became elongated and the number of microvilli decreased, but the number of rough endoplasmic reticulum increased. The level of AP-1 binding activity was increased by 79.22% after high glucose treatment, compared with that of normal glucose group, but decreased by 51.19% after the treatment of AP-1 inhibitor, compared with that in high glucose group. Cultured with high glucose, the expressions of CK mRNA and protein significantly decreased (P<0.05), but they significantly increased in AP-1 inhibited group (P<0.05). Upon the stimulation of high glucose, the expression of α-SMA protein increased markedly (P<0.05), while it significantly decreased in AP-1 inhibited group (P<0.05).      Conclusion    High glucose induces transdifferentiation of renal tubular cells probably through the activation of AP-1.

参考文献/References:

吕智美, 邓华聪, 诸葛福媛, 等. AP-1活化在高糖诱导肾小管上皮细胞转分化中的作用[J]. 第三军医大学学报,2008,30(22):2107-2110.

 

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更新日期/Last Update: 2008-10-30