[1]黄正根,吴军,罗高兴,等.胎猪皮肤前体组织异种移植模型的研究[J].第三军医大学学报,2008,30(22):2072-2074.
 HUANG Zheng-gen,WU Jun,LUO Gao-xing,et al.Xenotransplantation model of fetal pig skin precursor tissue[J].J Third Mil Med Univ,2008,30(22):2072-2074.
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胎猪皮肤前体组织异种移植模型的研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第22期
页码:
2072-2074
栏目:
论著
出版日期:
2008-11-30

文章信息/Info

Title:
Xenotransplantation model of fetal pig skin precursor tissue
作者:
黄正根吴军罗高兴甘成军程文广贾雄飞葛良鹏魏泓
第三军医大学:西南医院全军烧伤研究所,创伤、烧伤与复合伤国家重点实验室,重庆市器官移植基础研究所,重庆市疾病蛋白质组学重点实验室,基础医学部实验动物学教研室
Author(s):
HUANG Zheng-gen WU Jun LUO Gao-xing GAN Cheng-jun CHENG Wen-guang JIA Xiong-fei GE Liang-peng WEI Hong
State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Institute of Transplantation, Chongqing Key Laboratory for Proteomics of Diseases, Institute of Burns, Southwest Hospital, Department of Laboratory Animal, College of Medicine, Third Military Medical University, Chongqing 400038, China
关键词:
贵州香猪BALB/c裸鼠皮肤前体组织异种移植移植模型
Keywords:
Guizhou mini-pig BALB/c nude mice skin precursor tissue xenotransplantation transplantation model
分类号:
R-332;R73-352;R739.5
文献标志码:
A
摘要:
目的    探讨胎猪皮肤前体组织异种移植模型及移植后发育过程。    方法    取精确胎龄为56 d的胎猪皮肤前体组织,分组进行移植,①背部创面模型组:制备成微粒皮后移植到BALB/c裸鼠背部创面,以人尸体皮覆盖;背部皮下模型组:制备成小张邮票皮,植入裸鼠背部皮下;耳廓皮下模型组:制备成微粒皮植入裸鼠耳廓皮下。各组于移植后观察其生长发育特性,并于移植后6周和12周取材行HE染色观察其组织结构形态。新生组织块大小采用双样本t检验。    结果    各模型的胎猪皮肤前体组织移植后均能存活并继续生长,背部创面模型组生长能力显著大于耳廓皮下模型组,新生皮肤组织均具有真皮和表皮,耳廓皮下模型的真皮附属器仅见毛囊(毛发),偶见皮脂腺,未见汗腺,但是背部创面模型组及背部皮下模型组不仅能发育成毛囊(毛发)、还见大量皮脂腺和汗腺。    结论    背部创面移植模型最适合于胎猪皮肤前体组织异种移植及移植后发育的研究。
Abstract:
Objective    To investigate the xenotransplantation model of fetal pig skin precursor tissue and its development after transplantaion.     Methods    Porcine skin precursor tissue was obtained from the embryo of gestation day 56 (E56), and made into microskin or stamp skin graft. The microskin was transplanted to the dorsal wound in BALB/c nude mice, then covered with human corpse skin. The stamp skin graft was imbedded subcutaneously into the back of nude mice, and microskin was injected subcutaneously into the auricles of nude mice. Their growth and development were observed and they were examined by HE staining at 6th and 12th week after transplantation respectively. Two-sample  t  test was used to analyze the size of newly grown skin tissue.     Results    Porcine skin precursor tissue graft in three models above survived and continued growth after transplantation, and growth ability of the dorsal wound transplantation model was significantly stronger than that of the auricle model. Epidermis and hypodermis were detected in newly grown skin tissues. Hair follicles, a few of sebaceous glands, but no sweat glands were observed in auricle model, while many sebaceous glands and sweat glands were observed in the dorsal wound model.     Conclusion    Transplantation of microskin to dorsal wound is the optimal model of investigating the xenotransplantation of fetal pig skin precursor tissue and its development after transplantion.

参考文献/References:

黄正根,吴军,罗高兴,等. 胎猪皮肤前体组织异种移植模型的研究[J]. 第三军医大学学报,2008,30(22):2072-2074.

更新日期/Last Update: 2008-10-30