[1]黄河清,唐军,周振华,等.西酞普兰对血管性痴呆大鼠海马DG神经发生和认知障碍影响的初步研究[J].第三军医大学学报,2008,30(10):946-949.
 HUANG He-qing,TANG Jun,ZHOU Zhen-hua,et al.Effects of Citalopram on hippocampal neurogenesis and cognitive functions in rats of transient cerebral ischemia[J].J Third Mil Med Univ,2008,30(10):946-949.
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西酞普兰对血管性痴呆大鼠海马DG神经发生和认知障碍影响的初步研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第10期
页码:
946-949
栏目:
论著
出版日期:
2008-05-30

文章信息/Info

Title:
Effects of Citalopram on hippocampal neurogenesis and cognitive functions in rats of transient cerebral ischemia
作者:
黄河清唐军周振华李志方刘国军陈康宁徐海伟李露斯
第三军医大学:西南医院神经内科,基础医学部生理学教研室,重庆市神经科学研究所;中国人民解放军第65426部队
Author(s):
HUANG He-qing TANG Jun ZHOU Zhen-hua LI Zhi-fang LIU Guo-jun CHEN Kang-ning XU Hai-wei LI Lu-si
Department of Neurology, Southwest Hospital, Department of Physiology, College of Medicine, Third Military Medical University; Unit 65426 of People’s Liberation Army
关键词:
血管性痴呆海马齿回神经干细胞学习记忆
Keywords:
vascular dementia hippocampus neurogenesis cognitive function
分类号:
R329.28;R749.13;R971.43
文献标志码:
A
摘要:
目的  探讨西酞普兰对血管性痴呆大鼠海马齿回神经干细胞动员的作用及对动物空间认知行为的影响。  方法 实验动物采用随机数字表法分为正常对照组(NC组)、假手术对照组(SC组)、血管性痴呆组(4VO组)和西酞普兰干预的血管性痴呆组(4VO+C组)共4组;采用改良的Pulsinelli’s 四血管阻断(4-vessle occlusion, 4VO)方法制作血管性痴呆动物模型;BrdU免疫荧光和激光共聚焦方法观察海马齿回(dental gyrus, DG)神经干细胞增殖状况;Morris水迷宫检测大鼠空间学习和记忆功能。  结果  ①海马DG的BrdU阳性细胞多成对或成团排列于颗粒细胞层与海马门区。4VO组及4VO+C组造模后1 d,BrdU阳性细胞数显著下降,此后逐渐增加,到术后14 d达峰值(4VO+C组约为4VO组的2倍),4VO+C组DG神经干细胞活跃增生的情况一直延续到术后21 d,与4VO组比较差异显著(P<0.01)。②Morris水迷宫定位航行实验和空间探索实验均表明,造模后大鼠空间学习记忆功能显著受损,但随时间推移逐渐改善;4OV+C组术后7 d和14 d组大鼠的学习记忆损伤程度较4VO组显著减轻(P<0.05)。③大鼠海马DG神经干细胞增殖趋势与其空间学习记忆能力改变无明显相关性。  结论  全脑缺血再灌注可刺激大鼠海马DG神经干细胞增殖,且SSRI类抗抑郁药物西酞普兰可显著增强DG神经发生并减轻动物的空间学习记忆损伤,但神经干细胞增殖趋势与学习记忆改变无明显相关性,推测可能与增殖神经干细胞的分化、功能整合及神经递质作用等有关。
Abstract:
Objective  To investigate the neurogenetic function of Citalopram in rat hippocampus after transient cerebral ischemia and its effects on the animals’ cognitive function.   Methods  Totally 78 Wistar rats were randomly divided into four groups: normal control group (NC), sham operation control group (SC), transient cerebral ischemia group (4VO), transient cerebral ischemia group treated with Citalopram (4VO+C). The improved Pulsinelli’s 4-vessel occlusion was used to establish ischemia in rats. Neurogenesis in hippocampus was observed by BrdU (Bromodeoxyuridine) immunofluorescence and confocal laser scanning microscopy. The cognitive function of the rats was evaluated by Morris water maze test.   Results  Most of the BrdU-positive cells were detected in dentate gyrus, and generally in pairs or clusters, distributed at the border of the granule cell layer and hilus. One day after transient cerebral ischemia, the BrdU-positive cells obviously decreased in number in both 4VO and 4VO+C groups (P=0.002), then gradually increased and reached the peak 14 days after operation; The number of BrdU-positive cells in 4VO+C group was twice as much as that in 4VO group. Besides, neurogenesis was vividly continued 21 days after operation in 4VO+C group (P<0.01 compared with 4VO group). Both place navigation test and spatial probe test showed that the cognitive function was significantly injured by transient cerebral ischemia in 4VO group as well as in 4VO+C group rats. But as time went on, the cognitive function was improved. Furthermore the results of Morris water maze test were significantly better in 4VO+C group on 14th day and 21st day after operation than those corresponding results in 4VO group (P<0.05). The tendency of neurogenesis in adult dentate gyrus had no significant correlation with the cognitive function of rats.   Conclusion  Transient cerebral ischemia established by Pulsinelli’s 4-vessel occlusion in rats could stimulate neurogenesis in adult dentate gyrus and could be improved by Citalopram. Citalopram relieves the cognitive injury induced by transient cerebral ischemia. The differentiation and integration of proliferated neural stem cells or function of neurotransmitter may contribute to no correlation between hippocampal neurogenesis and cognitive function.

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更新日期/Last Update: 2008-05-27