[1]马玉霞,王琳,梁志清,等.去甲二氢愈创木酸治疗子宫内膜异位症的实验研究[J].第三军医大学学报,2008,30(11):1046-1048.
 MA Yu-xia,WANG Lin,LIANG Zhi-qing,et al.Effects of nordihydroguaiaretic acid on the endometriosis in Bama miniature pigs[J].J Third Mil Med Univ,2008,30(11):1046-1048.
点击复制

去甲二氢愈创木酸治疗子宫内膜异位症的实验研究(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第11期
页码:
1046-1048
栏目:
论著
出版日期:
2008-06-15

文章信息/Info

Title:
Effects of nordihydroguaiaretic acid on the endometriosis in Bama miniature pigs
作者:
马玉霞王琳梁志清孔燕
第三军医大学西南医院妇产科
Author(s):
MA Yu-xia WANG Lin LIANG Zhi-qing  KONG Yan
Department of Gynecology and Obstetrics, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
关键词:
血管生成抑制剂去甲二氢愈创木酸子宫内膜异位症
Keywords:
angiogenesis inhibitor nordihydroguaiaretic acid (NDGA) endometriosis
分类号:
R-332; R711.71; R977
文献标志码:
A
摘要:
目的  探讨血管生成抑制剂去甲二氢愈创木酸(nordihydroguaiaretic acid,NDGA)对子宫内膜异位症巴马香猪动物模型的影响。  方法  采用建模成功的巴马香猪6头,分为实验组NDGA组4头和对照组PBS组2头,实验组隔日皮下注射NDGA和对照组给予等体积的PBS溶液,连续给药20 d。观察给药前、后血清激素水平和异位内膜包块的变化情况,以及FⅧRAg和VEGF的表达情况。  结果  NDGA组血清E2显著降低(P<0.05)。NDGA组异位内膜包块不同程度的缩小,甚至少部分消失;PBS组包块无明显变化,部分略有增大。NDGA能显著抑制子宫内膜腺体数目减少,间质紧密;PBS组子宫腺体数目多,间质疏松。NDGA组减少微血管密度(MVD)的数目和VEGF的弱表达(P<0.05);PBS组微血管密度(MVD)的数目多和VEGF的强表达(P<0.05)。  结论 NDGA组种植的异位灶缩小甚至部分消失。
Abstract:
Objective    To investigate the effects of nordihydroguaiaretic acid (NDGA) on the endometriosis established in Bama miniature pigs.     Methods    Six Bama pigs that had been successfully established into animal model of endometriosis subcutaneously received 3% NDGA at dose of 20 mg/kg (n=4) or PBS solution (n=2) for 20 d. The grafts in abdominal cavity and subcutaneous tissues were observed and the serum concentrations of hormone,  were measured by chemiluminoimmunoassay before and after the period of NDGA or PBS injection. The expressions of FⅧAg and VEGF in the endometriotic tissues were observed.     Results    The endometriotic masses were reduced to some degree after the time period of NDGA injection, even partially disappeared, while those treated with PBS showed no obvious changes. Histoimmunochemical analysis showed NDGA decreased the number of endometrial glands, the proliferation rate of the glandular epithelial cells, the endometrial stroma cells and the vessels, and inhibited the microvascular density and VEGF in the endometriotic tissues (P<0.05). The serum concentration of E2 decreased significantly after the whole course of NDGA injection (P<0.05).     Conclusion    NDGA can reduce the endometriotic mass or even make partial endometriotic mass disappear.

参考文献/References:

马玉霞,王琳,梁志清,等. 去甲二氢愈创木酸治疗子宫内膜异位症的实验研究[J].第三军医大学学报,2008,30(11):1046-1048

相似文献/References:

[1]杨明福,刘广元,李前伟.99Tcm-MAG3-K237的制备及其在健康动物体内的药代动力学和分布研究[J].第三军医大学学报,2010,32(05):482.
 Yang Mingfu,Liu Guangyuan,Li Qianwei.Preparation of 99Tcm-MAG3-K237 and its pharmacokinetics and distribution in health animals[J].J Third Mil Med Univ,2010,32(11):482.
[2]卢佳友,卞修武,赵雯,等.NDGA诱导人胶质瘤细胞株分化过程中基因组甲基化及甲基转移酶的变化及意义[J].第三军医大学学报,2003,25(04):0.[doi:10.16016/j.1000-5404.2003.04.002 ]
 LU Jia you,BIAN Xiu wu,ZHAO Wen,et al.[J].J Third Mil Med Univ,2003,25(11):0.[doi:10.16016/j.1000-5404.2003.04.002 ]
[3]徐承平,卞修武,章容,等.去甲二氢愈创木酸对人恶性胶质瘤细胞系CHG-5生长的影响[J].第三军医大学学报,2004,26(08):0.[doi:10.16016/j.1000-5404.2004.08.006 ]
 XU Cheng ping,BIAN Xiu wu,ZHANG Rong,et al.[J].J Third Mil Med Univ,2004,26(11):0.[doi:10.16016/j.1000-5404.2004.08.006 ]
[4]孙慧勤,陈意生,史景泉,等.NDGA对内皮细胞增殖抑制的作用研究[J].第三军医大学学报,2000,22(03):0.[doi:10.16016/j.1000-5404.2000.03.018 ]
 SUN Hui qin,Chen Yi sheng,SHI Jing quan,et al.[J].J Third Mil Med Univ,2000,22(11):0.[doi:10.16016/j.1000-5404.2000.03.018 ]
[5]杜琳琳,陈意生,史景泉,等.血管生成抑制剂TNP470抗肿瘤作用的研究进展[J].第三军医大学学报,2000,22(09):0.[doi:10.16016/j.1000-5404.2000.09.039 ]
[6]郭德玉,陈意生,杜林林,等.去甲二氢愈创木酸对恶性胶质瘤细胞裸鼠移植瘤的作用[J].第三军医大学学报,1999,21(10):0.[doi:10.16016/j.1000-5404.1999.10.002 ]
 Guo Deyu,Chen Yisheng,Du Linlin,et al.[J].J Third Mil Med Univ,1999,21(11):0.[doi:10.16016/j.1000-5404.1999.10.002 ]
[7]姚莉,卞修武,张树辉,等.去甲二氢愈创木酸对恶性胶质瘤细胞基因组甲基化水平的影响及其意义[J].第三军医大学学报,2001,23(03):0.[doi:10.16016/j.1000-5404.2001.03.002 ]
 YAO Li,BIAN Xiu wu,ZHANG Shu hui,et al.[J].J Third Mil Med Univ,2001,23(11):0.[doi:10.16016/j.1000-5404.2001.03.002 ]
[8]姚莉,卞修武,陈自强,等.去甲二氢愈创木酸对SHG-44胶质瘤细胞GFAP基因表达的影响[J].第三军医大学学报,2001,23(03):0.[doi:10.16016/j.1000-5404.2001.03.004 ]
 YAO Li,BIAN Xiu wu,CHEN Zi qiang.[J].J Third Mil Med Univ,2001,23(11):0.[doi:10.16016/j.1000-5404.2001.03.004 ]
[9]郭德玉,陈意生,卞修武,等.CDK4在去甲二氢愈创木酸抑制恶性胶质瘤细胞增殖中的作用[J].第三军医大学学报,2001,23(03):0.[doi:10.16016/j.1000-5404.2001.03.005 ]
 GUO De yu,CHEN Yi sheng,BIAN Xiu wu,et al.[J].J Third Mil Med Univ,2001,23(11):0.[doi:10.16016/j.1000-5404.2001.03.005 ]
[10]郭德玉,陈意生,卞修武,等.bcl-2、c-myc基因表达在去甲二氢愈创木酸诱导人恶性胶质瘤细胞凋亡中的意义[J].第三军医大学学报,2001,23(03):0.[doi:10.16016/j.1000-5404.2001.03.006 ]
 GUO De yu,CHEN Yi sheng,BIAN Xiu wu,et al.[J].J Third Mil Med Univ,2001,23(11):0.[doi:10.16016/j.1000-5404.2001.03.006 ]
[11]吴晓华,王琳,史常旭.去甲二氢愈创木酸对大鼠卵巢功能的影响[J].第三军医大学学报,2005,27(10):1000.

更新日期/Last Update: 2008-06-06