[1]李骊华,雷寒.不同剂量厄贝沙坦对兔主动脉粥样硬化中环氧合酶2的影响[J].第三军医大学学报,2008,30(10):917-920.
 LI Li-hua,LEI Han.Effect of Irbesartan on cyclooxygenase-2 in aortic atherosclerotic lesion in rabbits[J].J Third Mil Med Univ,2008,30(10):917-920.
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不同剂量厄贝沙坦对兔主动脉粥样硬化中环氧合酶2的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第10期
页码:
917-920
栏目:
论著
出版日期:
2008-05-30

文章信息/Info

Title:
Effect of Irbesartan on cyclooxygenase-2 in aortic atherosclerotic lesion in rabbits
作者:
李骊华雷寒
重庆医科大学附属第一医院心血管内科
Author(s):
LI Li-hua LEI Han
Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University
关键词:
动脉粥样硬化 环氧合酶-2 厄贝沙坦
Keywords:
atherosclerosis cyclooxygenase-2 Irbesartan
分类号:
R446.6;R543.5;R972
文献标志码:
A
摘要:
目的   检测环氧合酶-2(COX-2)在兔主动脉粥样硬化(AS)中的表达,探讨血管紧张素 Ⅱ(AngⅡ)-1型受体拮抗剂厄贝沙坦(Irbesartan, Irb)对COX-2表达的影响及其可能的抗AS效应。  方法  健康雄性新西兰兔30只,按随机数字表法分为对照组、高胆固醇组(HC)、小剂量Irb(Sirb)组(10 mg·kg-1·d-1)、中剂量Irb(Mirb)组(20 mg·kg-1·d-1)和大剂量Irb(Lirb)组(30 mg·kg-1·d-1),每组6只。喂养14 周后,取各组兔主动脉,行AS斑块面积及主动脉内膜/中膜值测定,采用RT-PCR、免疫组化和Western blot法分别检测主动脉AS斑块中COX2 mRNA和蛋白的表达。  结果  Lirb和Mirb组AS斑块面积均较HC组显著缩小[分别为(14.84±2.51)%和(33.77±3.53)%比(61.74±4.33)%, P<0.01]。Lirb和Mirb组内膜/中膜值较HC组明显减小[分别为 (0.11±0.07)和(0.50±0.08)比(0.92±0.07), P<0.01]。HC组COX-2 mRNA的表达较对照组显著增强[(1.01±0.04)比(0.024±0.01),P<0.01];Lirb和Mirb干预后COX-2 mRNA的表达量明显下降[分别为(0.24±0.03)和(0.47±0.05),P<0.01]。14周时HC组主动脉AS斑块中COX-2蛋白表达明显增强,Mirb和Lirb干预后显著降低[免疫组化分别是(0.81±0.06)比(0.47±0.10)和(0.14±0.02)(P<0.01),Western blot分别是(1.10±0.05)比(0.89±0.02)和(0.51±0.04)(P<0.01)]。  结论  COX-2参与AS的发生和发展,中剂量和大剂量Irb均能降低AS斑块中COX-2的表达而抗AS,Irb对AS的有益作用可能是通过抑制COX-2表达实现的。
Abstract:
Objective  To study the effect of angiotensin Ⅱ type 1 receptor antagonist Irbesartan on the expressions of cyclooxygenase-2 (COX-2) in rabbit model of atherosclerosis.    Methods  Thirty male New Zealand rabbits were randomly divided into five groups: normal control group, high cholesterol group (Group HC), 10 mg·kg-1·d-1 Irbesartan group (Group S), 20 mg·kg-1·d-1 Irbesartan group (Group M), 30 mg·kg-1·d-1 Irbesartan group (Group H). Except the control group, the other four groups were fed with high cholesterol diet to induce atherosclerosis for 14 weeks. Then, the size of aortic atherosclerotic lesions and the ratio of aortic tunica intima to media were measured. RT-PCR, immunohistochemistry and Western blotting were used for COX-2 mRNA and protein levels respectively.    Results  Compared with Group HC, atherosclerotic lesion size was markedly reduced in Groups M and H (P<0.01). Compared with Group HC, the ratio of aortic tunica intima to media was significantly reduced in Groups H and M (P<0.01). COX-2 mRNA expression was significantly higher in Group HC than that of control group, groups H and M (P<0.01). COX-2 protein was higher in Group HC than that in Groups M and H (P<0.01).    Conclusion  COX-2 participates in the genesis and development of atherosclerosis. Irbesartan at certain dosage can decrease the expression of COX-2.

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更新日期/Last Update: 2008-05-26