[1]钟大平,李焱,周进明,等.Tiam1基因沉默降低结肠癌细胞VEGF-C/-D的表达[J].第三军医大学学报,2008,30(17):1607-1610.
 ZHONG Da-ping,LI Yan,ZHOU Jin-ming,et al.Silenced Tiam1 gene inhibits the expressions of VEGF-C/-D in colorectal cancer cells[J].J Third Mil Med Univ,2008,30(17):1607-1610.
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Tiam1基因沉默降低结肠癌细胞VEGF-C/-D的表达
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第17期
页码:
1607-1610
栏目:
论著
出版日期:
2008-09-15

文章信息/Info

Title:
Silenced Tiam1 gene inhibits the expressions of VEGF-C/-D in colorectal cancer cells
作者:
钟大平  李焱  周进明  周琪  姜友昭  杨启红  梁后杰
第三军医大学西南医院肿瘤科
Author(s):
ZHONG Da-ping LI Yan ZHOU Jin-ming ZHOU Qi JIANG You-zhao YANG Qi-hong LIANG Hou-jie
Department of Oncology, Southwest Hospital, Third military Medical University, Chongqing 400038, China
关键词:
 结肠癌Tiam1VEGF-C/-D
Keywords:
colorectal cancer Tiam1 VEGF-C/-D
分类号:
R394.3; R73-362; R735.35
文献标志码:
A
摘要:
目的    探讨T淋巴瘤侵袭转移诱导因子1(Tiam1)的表达与结肠癌淋巴管生成的关系。    方法    将实验细胞分为3组,分别为未干扰组(HCT116)、空质粒载体对照组(HCT116/CN)和干扰组(HCT116/siRNA-Tiam1)。应用siRNA使Tiam1基因表达下调,通过RT-PCR检测Tiam1 mRNA表达抑制率,并用RT-PCR和Western blot方法检测Tiam1基因干扰前后VEGF-C/-D mRNA和蛋白的表达情况。    结果    通过筛选后稳定表达的情况下,siRNA组的Tiam1、VEGF-C/-D mRNA和蛋白的表达明显低于未干扰组。    结论    Tiam1参与了VEGF-C/-D表达的调节,推测Tiam1可能通过诱导VEGF-C/-D的表达影响结肠癌淋巴管的生成。Tiam1可作为结肠癌淋巴转移过程中一个有价值的指标。
Abstract:
Objective    To study the relationship between Tiam1 gene and lymphangiogenesis in colorectal cancer.     Methods    Tiam1 was silenced by siRNA, then linked with pGenesil-EGFP1, finally transfected into the colorectal cancer cells HCT116. The cells without transfection and transfected with blank vector served as controls. The Tiam1 mRNA expressions were detected by RT-PCR. The expressions of VEGF-C/-D mRNA and protein were detected by RT-PCR and Western blot.     Results    The expressions of Tiam1 and VEGF-C/-D in HCT116 cells with Tiam1 gene silenced were lower than those in control groups.     Conclusion    Tiam1 can affect lymphangiogensis in colon cancer by its effect on VEGF-C/-D. It may be a useful marker for lymphatic metastasis in colorectal cancer.

参考文献/References:

钟大平,李焱,周进明,等.   Tiam1基因沉默降低结肠癌细胞VEGF-C/-D的表达[J]. 第三军医大学学报, 2008, 30(17):1607-1610.

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更新日期/Last Update: 2008-09-16