[1]张卫泽,樊艳,陈永清,等.血管紧张素Ⅱ诱导成人脂肪间充质干细胞分化为心肌样细胞的实验研究[J].第三军医大学学报,2008,30(19):1795-1798.
 ZHANG Wei-ze,FAN Yan,CHEN Yong-qing,et al.Angiotensin Ⅱ induces adult adipose-derived mesenchymal stem cells to differentiate into cardiocyte-like cells[J].J Third Mil Med Univ,2008,30(19):1795-1798.
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血管紧张素Ⅱ诱导成人脂肪间充质干细胞分化为心肌样细胞的实验研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第19期
页码:
1795-1798
栏目:
论著
出版日期:
2008-10-10

文章信息/Info

Title:
Angiotensin Ⅱ induces adult adipose-derived mesenchymal stem cells to differentiate into cardiocyte-like cells
作者:
张卫泽樊艳陈永清马凌哈小琴张耀辉
兰州军区兰州总医院心血管内科
Author(s):
ZHANG Wei-ze FAN Yan CHEN Yong-qing MA Ling HA Xiao-qin ZHANG Yao-hui
Department of Cardiology,  Lanzhou General Hospital of Lanzhou Military Command, Lanzhou 730050, China
关键词:
干细胞脂肪组织血管紧张素Ⅱ心肌细胞诱导分化
Keywords:
stem cells adipose tissue angiotensin Ⅱ myocardial cells induced differentiation
分类号:
R322.11; R392.1
文献标志码:
A
摘要:
目的    采用血管紧张素Ⅱ(angiotensin Ⅱ, AngⅡ)作为诱导剂,体外诱导成人脂肪间充质干细胞(adipose-derived mesenchymal stem cells, ADMSCs)向心肌细胞方向分化。    方法    自成人脂肪组织中分离培养ADMSCs,分别用0.05、0.10和0.20 μmol/L 3种不同浓度的AngⅡ诱导ADMSCs,同时设立对照组,倒置相差显微镜及透射电子显微镜下观察细胞的形态学特征,免疫细胞化学染色方法检测心肌特异性肌钙蛋白-I(cardiac troponin-I, cTn-I),计算转化率。    结果    诱导后第3周时,0.20 μmol/L组可见cTn-I阳性细胞表达,第4周时0.10 μmol/L组和0.20 μmol/L组均有cTn-I阳性细胞表达,但0.20 μmol/L组的阳性细胞数明显多于前组,且细胞形态变化显著。    结论    AngⅡ在体外可以诱导ADMSCs分化为心肌样细胞,其诱导分化的最佳浓度为0.20 μmol/L。
Abstract:
Objective    To study the effects of angiotensin Ⅱ (AngⅡ), as inducing agent in vitro, in inducing adult adipose-derived mesenchymal stem cells (ADMSCs) to the direction of myocardial cell.     Methods    ADMSCs were isolated and cultured from adult adipose tissues. Different concentrations of AngⅡ (0.05, 0.10 and 0.20  μmol/L, respectively) were used to induce ADMSCs. Untreated ADMSCs were used as normal control. The cell morphological feature was observed under inverted phase-contrast microscope and transmission electron microscope. Immunocytochemical staining was used to detect the cardiac troponin-I (cTn-I) and calculate the conversion rate.     Results    After three weeks of induction, cTn-I-positive cells could be detected in 0.20  μmol/L AngⅡ group. Four weeks later, cTn-I-positive cells were detected in 0.10 and 0.20  μmol/L AngⅡ groups, furthermore, the number of cTn-I-positive cells in 0.20  μmol/L AngⅡ group was significantly higher than that of other groups.     Conclusion    Ang Ⅱ can induce in vitro ADMSCs to differentiate into cardiocyte-like cells, and the best induction concentration is 0.20  μmol/L.

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更新日期/Last Update: 2008-09-25