[1]姚青,宋治远,马显光.脉冲微交流电刺激促进体外诱导大鼠骨髓间充质干细胞向心肌分化[J].第三军医大学学报,2008,30(05):410-413.
 YAO Qing,SONG Zhi-Yuan,MA Xian-guang.Electrical stimulation promotes the differentiation of rat bone marrow mesenchymal stem cells to cardiomyocyte induced by 5-azacytidine in vitro[J].J Third Mil Med Univ,2008,30(05):410-413.
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脉冲微交流电刺激促进体外诱导大鼠骨髓间充质干细胞向心肌分化(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第05期
页码:
410-413
栏目:
论著
出版日期:
2008-03-15

文章信息/Info

Title:
Electrical stimulation promotes the differentiation of rat bone marrow mesenchymal stem cells to cardiomyocyte induced by 5-azacytidine in vitro
作者:
姚青宋治远马显光
第三军医大学:西南医院心血管内科,重庆市介入心脏病学研究所,基础医学部物理学教研室
Author(s):
YAO Qing SONG Zhi-Yuan MA Xian-guang
Department of Cardiology,Southwest Hospital,Department of Physics, College of Medicine, Third Military Medical University
关键词:
电刺激骨髓间充质干细胞心肌样细胞
Keywords:
electrical stimulation bone marrow mesenchymal stem cells cardiomyocyte
分类号:
R322.11; R329.21; R337.5
文献标志码:
A
摘要:
目的 探讨脉冲微交流电刺激对体外诱导大鼠骨髓间充质干细胞向心肌样细胞分化的作用。 方法 常规方法分离培养鼠骨髓间充质干细胞,取P3细胞进行5-氮胞苷诱导,诱导后以是否给予脉冲微交流电刺激分为两组,观察各组细胞生长情况、细胞形态学及超微结构改变,免疫细胞化学方法检测α-actin、cTnT、Cx43的表达。 结果 脉冲微交流电刺激组细胞较非刺激组生长良好,更早出现心肌样细胞改变,诱导后1周可观察到胞内肌丝形成及α-actin、cTnT、Cx43的表达,且在随后的相同时相点(诱导后2、3、4周),α-actin、cTnT、Cx43表达水平明显高于非刺激组,胞内肌丝也由杂乱分布逐渐成束,部分细胞可观察到肌节。 结论 模拟心脏跳动的脉冲微交流电刺激有助于经5-氮胞苷诱导的骨髓间充质干细胞在体外分化为功能完备的心肌样细胞。
Abstract:
Objective To investigate the effects of electrical stimulation on the differentiation of rat bone marrow mesenchymal stem cells (MSCs) to cardiomyocyte induced by 5-azacytidine in vitroMethods MSCs from Sprague-Dawley (SD) rats were cultured and passed repeatedly to P3. MSCs were treated with 5-azacytidine (10 μmol/L) and incubated for 24 h.The induced MSCs were divided to stimulated group and non-stimulated group, and every groups divided by incubated for 1,2,3 and 4 weeks were named as subgroup Ⅰ,Ⅱ, Ⅲ and Ⅳ respectively.MSCs of stimulated group were stimulated for 30 min every day by supra-threshold square biphasic pulses (2 ms duration, 1.5 Hz, 20 μA), and the stimulation was initiated 1 d after inducing. Light and electronic microscope were used to identify the influences of characteristic morphological of MSCs in every subgroups,and immunocytochemistry was used to identify the expression of α-actin,cTnT, Cx43 in MSCs.  Results The growth of MSCs in stimulated group was better than that of non-stimulated group. MSCs of stimulated group exhibited differentiation into cardiomyocyte-like cell at 1 week after inducing, earlier than that of no-stimulated group (2 weeks). In stimulated subgroup Ⅰ, scattered myogenic structure was observed in the plasma of some cells under electronic microscope, and α-actin,cTnT expressed in some cells, but not that be observed in non-stimulated subgroup Ⅰ. In cells of stimulated subgroups Ⅱ to Ⅳ, the expression level of α-actin, cTnT, Cx43 of were all higher than that of non-stimulated subgroups respectively.  Conclusion Electrical stimulation (simulating the heart beating) could redound differentiation of rat bone marrow mesenchymal stem cells (MSCs) to cardiomyocyte induced by 5-azacytidine in vitro.

参考文献/References:

姚青, 宋治远, 马显光. 脉冲微交流电刺激促进体外诱导大鼠骨髓间充质干细胞向心肌分化[J]. 第三军医大学学报,2008,30(5):410-413.

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更新日期/Last Update: 2008-04-22