[1]蒋清安,何国祥,刘建平,等.一种新的非人工通气兔心肌梗死模型的制作[J].第三军医大学学报,2008,30(05):406-409.
 JIANG Qing-an,HE Guo-xiang,LIU Jian-ping,et al.A novel rabbit model of myocardial infarction without endotracheal intubation[J].J Third Mil Med Univ,2008,30(05):406-409.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第05期
页码:
406-409
栏目:
论著
出版日期:
2008-03-15

文章信息/Info

Title:
A novel rabbit model of myocardial infarction without endotracheal intubation
作者:
蒋清安何国祥刘建平苗莉景涛
第三军医大学西南医院心血管内科,重庆市介入心脏病学研究所
Author(s):
JIANG Qing-an HE Guo-xiang LIU Jian-ping MIAO Li JING Tao
Department of Cardiology, Southwest Hospital, Third Military Medical University
关键词:
家兔疾病模型心肌梗死非人工通气
Keywords:
rabbits myocardial Infarction disease modelsintubation
分类号:
R-332; R542.22
文献标志码:
A
摘要:
目的 在非人工通气的情况下建立心肌梗死实验动物模型。 方法b15只健康中国家兔非人工通气,经胸骨正中切口剪开胸骨和心包。其中10只于左室主支起点与心尖连线的中点位置结扎左冠脉左室支,作为冠脉结扎组;另5只开胸后假结扎冠脉作为假结扎组。4周后处死,观测其心电图和心脏组织学特征、去甲肾上腺素激发实验分析心功能。 结果 与冠脉假结扎组比较,冠脉结扎组其ECG和组织学特征均表现为心肌梗死;评定心脏收缩功能的指标LVSP、+dp/dtmax和t-dp/dtmax,冠脉结扎组降低和缩短;评定心脏舒张功能的指标于冠脉结扎组 LVEDP升高,而-dp/dtmax降低。 结论 兔心肌梗死模型的制作可以通过非人工通气、有效、简便和实用的方法建立。
Abstract:
Objective To develop a myocardial infarction model in rabbits without endotracheal intubation.  Methods A total of 15 Chinese rabbits of both sexes and (2.18±0.19)kg in weight were subjected. The sternum was carefully incised along the midline to avoid injury to the parietal pleura without endotracheal intubation. The major branch of the left coronary artery of 10 rabbits used as coronary artery ligation group was then ligated. Five rabbits with sham ligating the coronary artery were used as control. After 4 weeks, cardiac functional analyses by noradrenalin challenge and histopathological analyses were performed.  Results Compared with the coronary artery sham-ligation group, the coronary artery ligation group all presented to myocardial infarction through ECG and histologic characteristics. Cardiac functional analyses by noradrenalin after 4 weeks showed the parameters on cardiac systolic function in the coronary artery ligation group got worse significantly, that is to say LVSP and +dp/dtmax increased, while t-dp/dtmax shorten. Meanwhile, cardiac diastolic function got worse significantly. LVEDP rose, and -dp/dtmax dropped.  Conclusion A new method established a chronic rabbit model of myocardial infarction without the need for endotracheal intubation.

参考文献/References:

蒋清安, 何国祥, 刘建平,等. 一种新的非人工通气兔心肌梗死模型的制作[J]. 第三军医大学学报,2008,30(5):406-409.

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更新日期/Last Update: 2008-04-22