[1]杨启红,徐强,张红,等.糖基化终产物对THP-1巨噬细胞酰基辅酶A:胆固醇酰基转移酶-1表达的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(17):1630-1633.
 YANG Qi-hong,XU Qiang,ZHANG Hong,et al.Effects of advanced glycation end-products on expression of acyl coenzyme A: cholesterol acyltransferase-1 in THP-1 macrophages[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(17):1630-1633.
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糖基化终产物对THP-1巨噬细胞酰基辅酶A:胆固醇酰基转移酶-1表达的影响(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第17期
页码:
1630-1633
栏目:
论著
出版日期:
2008-09-15

文章信息/Info

Title:
Effects of advanced glycation end-products on expression of acyl coenzyme A: cholesterol acyltransferase-1 in THP-1 macrophages
作者:
杨启红徐强张红司良毅
第三军医大学西南医院老年病科;    解放军第113医院老年病科
Author(s):
YANG Qi-hong XU Qiang ZHANG Hong SI Liang-yi
Department of Geriatrics, Southwest Hospital, Third Military Medical University, Chongqing 400038; Department of Geriatrics, No.113 Hospital of PLA, Ningbo Zhejiang 315021, China
关键词:
 糖尿病糖基化终产物酰基辅酶A:胆固醇酰基转移酶-1基因表达
Keywords:
diabetes advanced glycation end products acyl coenzyme A: cholesterol acyltransferase-1 gene expression
分类号:
R392.12; R392.6; R587.102
文献标志码:
A
摘要:
目的    观察糖基化终产物(advanced glycosylation end-products, AGEs)对THP-1巨噬细胞酰基辅酶A:胆固醇酰基转移酶-1(acyl coenzyme A: cholesterol acyltransferase-1, ACAT-1)表达的影响。    方法    将糖基化-牛血清白蛋白(AGE-modified bovine serum albumin, AGE-BSA)与佛波酯(PMA)诱导分化72h的THP-1巨噬细胞共同孵育,运用RT-PCR和Western blot分别检测巨噬细胞ACAT-1 mRNA和蛋白的表达水平。    结果    PMA诱导72 h后,THP-1细胞停止增殖,由单核细胞分化成为巨噬细胞。用50、100、200和400 mg/L AGE-BSA处理巨噬细胞后,细胞ACAT-1 mRNA相对表达量逐渐增加;ACAT-1蛋白表达的平均积分光密度值也逐渐增加,二者较对照牛血清白蛋白组明显增加(P<0.05)。用200 mg/L AGE-BSA作用巨噬细胞0、12、24、36 h和48 h后,细胞ACAT-1 mRNA相对表达量逐渐升高;ACAT-1蛋白表达的平均积分光密度值也逐渐升高,较0 h明显增加(P<0.05)。    结论    AGEs可上调THP-1巨噬细胞ACAT-1 mRNA和蛋白的表达,呈浓度和时间依赖性,这可能与糖尿病动脉粥样硬化有关。
Abstract:
Objective    To investigate the effects of advanced glycosylation end-products (AGEs) on the expression of acyl coenzyme A: cholesterol acyltransferase-1 (ACAT-1) in cultured THP-1 macrophages.      Methods    THP-1 cells were differentiated into macrophages after cultured in RPMI1640 media containing 0.1 μmol/L PMA for 72 h. THP-1 macrophages were then exposed to AGE-modified bovine serum albumin (AGE-BSA, at concentration of 50, 100, 200 mg/L) for 24 h or to 200 mg/L AGE-BSA for 0, 12, 24, 36 h. Expression of ACAT-1 mRNA and protein in THP-1 macrophages was measured by RT-PCR and Western blot, respectively.      Results    After induced by 0.1 μmol/L PMA, THP-1 cells stopped proliferation and differentiated into macrophages. Treatment of THP-1 macrophages with AGE-BSA resulted in an increase in the mRNA and protein levels for ACAT-1 in a dose- and time-dependent manner.     Conclusion    AGEs upregulate the expression levels of mRNA and protein for ACAT-1 in cultured THP-1 macrophages, which might be partly involved in the atherogenesis in diabetic patients.

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更新日期/Last Update: 2008-09-16