[1]余祖滨,白莉,姚珂,等.SOCS3对肺腺癌细胞株A549迁移和侵袭能力的影响[J].第三军医大学学报,2008,30(07):588-591.
 YU Zu-bin,BAI Li,YAO Ke,et al.Effects of SOCS3 gene on migration and invasion of lung adenocarcinoma cell line A549[J].J Third Mil Med Univ,2008,30(07):588-591.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第07期
页码:
588-591
栏目:
论著
出版日期:
2008-04-15

文章信息/Info

Title:
Effects of SOCS3 gene on migration and invasion of lung adenocarcinoma cell line A549
作者:
余祖滨白莉姚珂张国强闵家新肖颖彬
第三军医大学新桥医院:胸外科,全军呼吸内科研究所,全军呼吸病研究重点实验室,全军心血管外科中心
Author(s):
YU Zu-bin BAI Li YAO Ke ZHANG Guo-qiang MIN Jia-xin XIAO Ying-bin
Department of Thoracic Surgery, Institute of Respiratory Diseases, Department of Cardiovascular Surgery, Xinqiao Hospital, Third Military Medical University
关键词:
肺腺癌侵袭转移SOCS
Keywords:
lung adenocarcinoma invasion migration SOCS
分类号:
R394.2; R730.23; R734.2
文献标志码:
A
摘要:
目的 观察细胞因子信号负调控因子3(suppressors of cytokine signaling, SOCS3)对人肺腺癌细胞株A549迁移和侵袭能力的影响,并初步探讨其机制。 方法 建立SOCS3稳定表达细胞株;Boyden Chamber分析法测定细胞的迁移和侵袭能力;半定量RT-PCR检测细胞基质金属蛋白酶(matrix metalloproteinases, MMPs)2,9 mRNA表达;明胶酶谱分析法测定MMP2,9蛋白活性水平。 结果 SOCS3稳定表达株细胞迁移和侵袭能力显著下降 (P<0.05);半定量RT-PCR和明胶酶谱分析结果显示SOCS3可显著抑制MMP2,9表达和活性 (P<0.05)。 结论 SOCS3能通过抑制MMP2,9表达和活性明显降低A549的体外迁移和侵袭能力。
Abstract:
Objectiv To explore the mechanism of SOCS3 on the migration and invasion of human lung adenocarcinoma cell line A549. Methods A549 cells that express SOCS3 stably were generated. The migration and invasion abilities of A549 cells were observed by Boyden Chamber assay. The mRNA and protein expressions of MMP-2 and MMP-9 were assessed by semi-quantitive RT-PCR and gelatin zymography respectively. Results Compared with the control group, the migration and invasion abilities of A549 cells with stable SOCS3 expression were significantly lower (P<0.05). The mRNA expression and activities of MMP-2 and MMP-9 in A549 cells with stable SOCS3 expression were inhibited significantly (P<0.05). Conclusion SOCS3 protein inhibits the migration and invasion abilities of A549 cells probably by down-regulating the expression and activation of MMP-2 and MMP-9.

参考文献/References:

余祖滨,白莉,姚珂,等. SOCS3对肺腺癌细胞株A549迁移和侵袭能力的影响[J]. 第三军医大学学报,2008,30(7):588-591.

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更新日期/Last Update: 2008-04-16