[1]王富友,杨柳,段小军,等.人体正常膝关节钙化软骨层组成成分研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(08):687-690.
 WANG Fu-you,YANG Liu,DUAN Xiao-jun,et al.Study on the components of calcified cartilage zone in the normal human knee joint[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(08):687-690.
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人体正常膝关节钙化软骨层组成成分研究(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第08期
页码:
687-690
栏目:
论著
出版日期:
2008-04-30

文章信息/Info

Title:
Study on the components of calcified cartilage zone in the normal human knee joint
作者:
王富友杨柳段小军戴刚谭洪波郭林陈光兴李忠
第三军医大学西南医院关节外科中心
Author(s):
WANG Fu-you YANG Liu DUAN Xiao-jun DAI Gang TAN Hong-bo GUO Lin CHEN Guang-xing LI Zhong
Center for Joint Surgery, Southwest Hospital, Third Military Medical University
关键词:
关节软骨钙化软骨层成分分析X-射线衍射
Keywords:
articular cartilage calcified cartilage zone component analysis Xray diffraction
分类号:
R322.72;R329;R446.8
文献标志码:
A
摘要:
目的 分析构成关节软骨钙化层有机成分中胶原蛋白类型与含量及无机成分中羟基磷灰石含量。 方法 组织库获取20例自愿捐献的人体正常股骨髁新鲜标本,组织切片采用天狼猩红染色和免疫组织化学对胶原蛋白进行定性分析;用氨基酸分析和X-射线衍射法分别对胶原蛋白和羟基磷灰石进行定量分析。 结果 天狼猩红染色见钙化软骨层与透明软骨层一样黄绿色淡染、弱双折光性;Ⅱ型胶原蛋白免疫组织化学分析,钙化软骨层呈强绿色荧光,潮线附近的胶原蛋白呈板层平行分布;氨基酸定量分析钙化软骨层Ⅱ型胶原蛋白含量占组织干质量的(20.16%±0.96%);X-射线衍射定量分析钙化软骨层中的羟基磷灰石含量占无机成分的(65.09%±2.31%)。 结论 在关节骨软骨复合组织中,钙化软骨层是位于透明软骨层与软骨下骨之间的界面连接结构,其细胞外基质主要由Ⅱ型胶原蛋白和羟基磷灰石组成。
Abstract:
Objective To analyze the type and contents of collagen protein in the organic principle of calcified cartilage zone, and the contents of hydroxyapatite in its inorganic composition.  Methods The normal human condyles of femur (n=20) from tissue bank were donated by the residents of Chongqing city. Their paraffin-embedded sections were prepared after fixation and decalcification. The type of collagen protein in the organic principle of calcified cartilage zone was qualitatively analyzed with the Picrosirius-polarization method and collagen Ⅱ protein with immunohistochemistry analysis, and the contents of collagen protein and hydroxyapatite were quantitatively analyzed with amino acid analysis and X-ray diffraction.  Results Same as hyaline cartilage, the calcified cartilage was stained light flavo-green and weak double refraction by the Picrosirius-polarization method. In the immunohistochemistry analysis of collagen Ⅱ protein, the calcified cartilage was stained strong green fluorescence and the collagen protein parallelled in joint surface near the tidemark. The content of collagen Ⅱ protein was (20.16%±0.96%) of tissue dry weight by amino acid analysis, and that of the hydroxyapatite was (65.09%±2.31%) of the inorganic composition in calcified cartilage.  Conclusion The calcified cartilage zone locates between the hyaline cartilage zone and subchondral bone in the complex tissues of articular cartilage and is mainly composed of collagen Ⅱ protein and hydroxyapatite.

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更新日期/Last Update: 2008-04-28